Crateva adansonii DC, an African ethnomedicinal plant, exerts cytotoxicity in vitro and prevents experimental mammary tumorigenesis in vivo

Zingue, Stéphane; Cisilotto, Júlia; Tueche, Alain Brice; Bishayee, Anupam; Mefegue, Francine Azegha; Sandjo, Louis P.; Nde, Chantal Beatrice Magne; Winter, Evelyn; Michel, Thomas; Ndinteh, Derek Tantoh; Awounfack, Charline Florence; Silihe, Kevine Kamga; Tanekou, Tito Trésor Melachio; Creczynski-Pasa, Tânia Beatriz; Njamen, Dieudonné

doi:10.1016/j.jep.2016.06.004

Cited by 27 publications

(24 citation statements)

References 63 publications

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“…However, the histopathological analysis of mammary section revealed ductal hyperplasia in rats exposed to DMBA and F. umbellata AE extract (50 mg/kg) treated rats. These results are in line with numerous scientific reports, which showed that DMBA alters the normal process of mammary gland differentiation [40,41,42]. Animal treated with F. umbellata AE (200 mg/kg) and MeOH (50 mg/kg) extracts as well as tamoxifen were protected against mammary glands hyperplasia.…”

Section: Discussionsupporting

confidence: 91%

Ficus umbellata Vahl. (Moraceae) Stem Bark Extracts Exert Antitumor Activities In Vitro and In Vivo

Silihe

Zingue

Winter

et al. 2017

IJMS

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A Ficus umbellata is used to treat cancer. The present work was therefore designed to assess antitumor potentials of F. umbellata extracts in nine different cell lines. Cell cycle, apoptosis, cell migration/invasion, levels of reactive oxygen species (ROS), mitochondrial membrane potential (MMP), caspases activities as well as Bcl-2 and Bcl-xL protein content were assessed in MDA-MB-231 cells. The 7,12-dimethylbenz(a)anthracene (DMBA)-induced carcinogenesis in rats were also used to investigate antitumor potential of F. umbellata extracts. The F. umbellata methanol extract exhibited a CC50 of 180 μg/mL in MDA-MB-231 cells after 24 h. It induced apoptosis in MCF-7 and MDA-MB-231 cells, while it did not alter their cell cycle phases. Further, it induced a decrease in MMP, an increase in ROS levels and caspases activities as well as a downregulation in Bcl-2 and Bcl-xL protein contents in MDA-MB-231 cells. In vivo, F. umbellata aqueous (200 mg/kg) and methanol (50 mg/kg) extracts significantly (p < 0.001) reduced ovarian tumor incidence (10%), total tumor burden (58% and 46%, respectively), average tumor weight (57.8% and 45.6%, respectively) as compared to DMBA control group. These results suggest antitumor potential of F. umbellata constituents possibly due to apoptosis induction mediated through ROS-dependent mitochondrial pathway.

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Section: Discussionsupporting

confidence: 91%

Ficus umbellata Vahl. (Moraceae) Stem Bark Extracts Exert Antitumor Activities In Vitro and In Vivo

Silihe

Zingue

Winter

et al. 2017

IJMS

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“…17 The dichloromethane/ methanol (DCM/MeOH) stem barks extract of C adansonii induced cytotoxicity in estrogen receptor-positive breast cancer cell line MCF-7 (CC 50 = 289 μg/mL) and protected female Wistar rats against 7,12-dimethylbenz(a)anthracene (DMBA)-induced mammary tumor. 18 This extract also exhibited in vitro and in vivo antiestrogenic effects on ovariectomized Wistar rats. 19 Furthermore, daucosterol, a natural phytosterol compound, was identified and isolated from C adansonii DCM/MeOH extract as its major bioactive ingredient using a bioassay-guided fractionation.…”

mentioning

confidence: 88%

“…Effects of daucosterol on transaminases, lipidemia, and creatinine by an increase in SOD and catalase activities. 18 These belong to the family of antioxidant enzymes, the primary line of defense against ROS in the biological system. 42 The SOD is a major intracellular enzyme, which protects against free radicals by catalyzing the SOD free radicals and anion to hydrogen peroxide and oxygen, which is further removed by catalase and glutathione peroxidase.…”

Section: T a B L Ementioning

confidence: 99%

Daucosterol from Crateva adansoniiDC (Capparaceae) reduces 7,12‐dimethylbenz(a)anthracene‐induced mammary tumors in Wistar rats

Nguedia

Tueche

Yaya

et al. 2020

Environmental Toxicology

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This study aimed to evaluate the in vivo anticancer effects of daucosterol which was earlier reported to possess in vitro anticancer effects. Breast tumor was induced in 30 rats using the environmental carcinogen 7,12‐dimethylbenz(a)anthracene (DMBA) while 6 control rats received olive oil (NOR). Animals with palpable tumors were randomized into five groups (n = 6) each as follows: negative control group treated with the vehicle (DMBA); positive control group treated with 5 mg/kg BW doxorubicin (DOXO + DMBA); three groups treated with daucosterol at doses of 2.5, 5, and 10 mg/kg BW (DAU + DMBA). Treatment lasted 28 days afterward, tumor (mass, volume, cancer antigen [CA] 15‐3 level and histoarchitecture), hematological and toxicological parameters were examined. The tumor volume gradually increased in the DMBA group during the 28 days, with a tumor volume gain of ∼390 cm3. Daucosterol at all doses reduced tumor volume (∼133.7 cm3 at 10 mg/kg) as well as protein, malondialdehyde (MDA), and CA 15‐3 levels compared to DMBA rats. Tumor sections in daucosterol‐treated rats showed a lower proliferation of mammary ducts with mild (5 and 10 mg/kg) to moderate (2.5 mg/kg) inflammatory responses. Moreover, it exhibited an antioxidant effect, evidenced by a significant and dose‐dependent decreased in MDA levels, as well as an increase in catalase activity compared to the DMBA group. Daucosterol showed for the first time in vivo antitumor effects that corroborate its previous in vitro effects.

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“…Ainsi, cette plante a été indiquée dans le traitement des maladies telles que l'abcès, la constipation, la diarrhée, l'hypertension artérielle et le paludisme (Agbankpé et al, 2015). En dehors des maladies citées par les enquêtés, les activités antitumorales de C. adansonii ont été prouvées par une étude ethnomédicinale (Zingue et al, 2016).…”

Section: Utilisation De C Edulis Et C Adansoniiunclassified

Connaissances ethnobotaniques et valorisation de deux plantes antihypertensives (<i>Carissa edulis</i> L. et <i>Crateva adansonii</i> DC) au Sud et au Centre du Bénin (Afrique de l’Ouest)

Olou¹,

Bio²,

Koko³

et al. 2019

Int. J. Bio. Chem. Sci

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La prévalence de l'hypertension artérielle (HTA) augmente sans cesse et sa prise en charge par la médecine moderne devient de plus en plus onéreuse. La présente étude vise une analyse des connaissances des populations sur deux plantes (Carissa edulis et Crateva Adansonii) qui interviennent dans le traitement traditionnel de cette maladie en vue de leur valorisation. A cet effet, les connaissances ethnobotaniques issues de chacune de ces plantes ont été recensées à l'aide d'enquêtes semi-structurée et individuelle. Les résultats ont montré que les populations locales, dans leur grande majorité, ne connaissent pas toutes les utilisations faites de ces plantes. Ces deux plantes ont en commun l'usage médicinal, alimentaire et fourrager. A ces trois usages, s'ajoute l'usage médico-magique pour C. adansonii. Elles sont toutes utilisées contre l'HTA mais, C. edulis est principalement utilisée contre la faiblesse sexuelle (IF = 100%) et C. adansonii contre l'abcès (IF = 100%). Dans la préparation des remèdes traditionnels contre l'HTA, la racine de C. edulis et la feuille de C. adansonii sont sollicitées. En tenant compte de cette connaissance, un produit antihypertensif a été mis en place. Il est nommé "Thé-HTA".

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Crateva adansonii DC, an African ethnomedicinal plant, exerts cytotoxicity in vitro and prevents experimental mammary tumorigenesis in vivo

Cited by 27 publications

References 63 publications

Ficus umbellata Vahl. (Moraceae) Stem Bark Extracts Exert Antitumor Activities In Vitro and In Vivo

Ficus umbellata Vahl. (Moraceae) Stem Bark Extracts Exert Antitumor Activities In Vitro and In Vivo

Daucosterol from Crateva adansoniiDC (Capparaceae) reduces 7,12‐dimethylbenz(a)anthracene‐induced mammary tumors in Wistar rats

Connaissances ethnobotaniques et valorisation de deux plantes antihypertensives (<i>Carissa edulis</i> L. et <i>Crateva adansonii</i> DC) au Sud et au Centre du Bénin (Afrique de l’Ouest)

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