Creatine Activates Airway Epithelium in Asthma

Abstract

TAirway epithelium plays important roles in the pathophysiology of asthma. Creatine supplementation (Cr) was shown to increase asthma features in a murine model of allergic asthma; however, the role of the airway epithelium in this infiammatory response is not known. BALB/c mice were divided into control, creatine supplementation, ovalbumin-sensitized (OVA) and OVA plus creatine supplementation groups. OVA sensitization occurred on days 0, 14, 28 and 42, and ovalbumin challenge from days 21-53. Cr was also given on days 21-53. Total and differential cells counts in BALF were evaluated. Quantitative epithelial expression of interleukin (IL)-4,

Creatine supplementation attenuates pulmonary and systemic effects of lung ischemia and reperfusion injury

“…Cr supplementation became a popular ergogenic aid to increase muscle performance, 12 with more than 2,500 metric tons used in 2000. 33 Although Cr displays several beneficial therapeutic effects, 2,[12][13][14][15][16]20,21,34 other studies have also found that Cr supplementation does not have a beneficial effect on the respiratory system during respiratory diseases. 15,[35][36][37] This study, however, indicates that Cr supplementation presents beneficial effects after lung I/R injury, including improvements in lung mechanics and inflammation.…”

“…16,32 The measurements were performed in 5 entire pulmonary artery walls and in 5 entire bronchial walls of each rat at original magnification Â400 in a blinded fashion. The results were expressed as percentage of positive-stained area in relation to the total area of the artery wall and of the bronchial epithelium.…”

“…The results were expressed as percentage of positive-stained area in relation to the total area of the artery wall and of the bronchial epithelium. 16,32 Western blotting…”

“…12 Cr supplementation also presents prophylactic and therapeutic effects for some muscular, neuromuscular, cardiovascular, and neurologic disorders. 12 Previous studies have demonstrated that Cr supplementation increases the expression of insulin-like growth factor 1 (IGF-1) in skeletal muscle 13,14 and in lungs 15,16 and modulates the expression of TLRs. 17 Cr supplementation reduces I/R injury in the brain and heart.…”

Efeitos da suplementação com creatina na lesão de isquemia e reperfusão após transplante pulmonar unilateral em ratos

“…Os animais doadores receberam creatina (Sigma®, 0,5g/kg/dia) (Vieira et al, 2007;Vieira et al, 2009;Ferreira et al, 2010;Almeida et al, 2016) diluída em 1 mL de água filtrada, por gavagem (figura 1A), durante 5 dias antes do procedimento cirúrgico.…”