Creatine as an antioxidant

Sestili, Piero; Martinelli, Chiara; Colombo, Evelin; Barbieri, Elena; Potenza, Lucia; Sartini, Stefania; Fimognari, Carmela

doi:10.1007/s00726-011-0875-5

Cited by 166 publications

(124 citation statements)

References 88 publications

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“…In postmortem muscles, depletion of ATP due to anoxia leads to the utilization of phosphocreatine to produce ATP and creatine. Previous studies have indicated that creatine can also act as an antioxidant by scavenging the free radicals (Sestili et al, 2011;Lawler et al, 2002). Creatine kinase M-type was more abundant (P < 0.05) in LL and ST, and these muscles had better color stability compared to PM.…”

Section: Proteins Related To Energy Metabolismmentioning

confidence: 87%

Changes in the Sarcoplasmic Proteome of Beef Muscles with Differential Color Stability during Postmortem Aging

Nair

Beach

et al. 2018

Meat and Muscle Biology

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Beef color is a muscle-specific trait, and sarcoplasmic proteome influences muscle-specific variations in beef color stability. Postmortem aging influences the color and sarcoplasmic proteome of beef muscles. Nonetheless, muscle-specific changes in sarcoplasmic proteome of beef muscles with differential color stability during aging have not been characterized yet. Therefore, our objective was to examine the changes in the sarcoplasmic proteome of 3 differentially color stable muscles from beef hindquarters during postmortem aging. Longissimus lumborum (LL), psoas major (PM), and semitendinosus (ST) separated from 8 (n = 8) beef carcasses (24 h postmortem) were subjected to aging in vacuum packaging (2°C) for 0, 7, 14, and 21 d. On each aging day, steaks were fabricated, and allotted to refrigerated storage (2°C) under aerobic packaging. Samples for proteome analysis obtained during fabrication were frozen at –80°C. Instrumental color and metmyoglobin reducing activity were evaluated on d 0, 3, and 6 of storage. Sarcoplasmic proteome was analyzed, and differentially abundant proteins were identified using mass spectrometry. Color attributes and biochemical parameters were influenced by muscle source and aging (P < 0.05); LL and ST had greater (P < 0.05) surface redness than PM. Aging also influenced surface redness, with 7-d aged steaks demonstrating greatest values (P < 0.05). Proteome analysis identified 135 protein spots differentially abundant (P < 0.05) between the muscles and aging time points indicating muscle-specific changes during aging. The identified proteins included glycolytic enzymes, proteins associated with energy metabolism, antioxidant proteins, chaperones, and transport proteins. Overall, the glycolytic enzymes were more abundant (P < 0.05) in color-stable muscles and at aging times with greater color stability, indicating that these proteins could be used as potential biomarkers for beef color.

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Section: Proteins Related To Energy Metabolismmentioning

confidence: 87%

Changes in the Sarcoplasmic Proteome of Beef Muscles with Differential Color Stability during Postmortem Aging

Nair

Beach

et al. 2018

Meat and Muscle Biology

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“…Creatine phosphate is utilized for the production of ATP with concomitant production of creatine in postmortem skeletal muscles. Previous research indicated that creatine has antioxidant properties (Sestili et al, 2011;Lawler et al, 2002), which could be beneficial to minimize myoglobin oxidation in fresh meat. In the present study, 2 spots identified as creatine kinase were more abundant in color-stable OSM21, which indicated the antioxidant effect of this protein in fresh beef.…”

Section: Sarcoplasmic Proteome Variations Between Ism and Osm Aged Fomentioning

confidence: 99%

Intramuscular Variations in Color and Sarcoplasmic Proteome of Beef Semimembranosus during Postmortem Aging

Nair

Beach

et al. 2018

Meat and Muscle Biology

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Abstract:Beef semimembranosus exhibits intramuscular difference in color stability, and the inside region (ISM) of the muscle is color-labile, whereas the outside region (OSM) is color-stable. Variations in sarcoplasmic proteins are known to contribute to this intramuscular color difference. Sarcoplasmic proteome and beef color are affected by postmortem aging. The objective of the present study was to examine the effect of aging on intramuscular color variations and the sarcoplasmic proteome of beef semimembranosus. Semimembranosus muscles obtained from 8 beef carcasses (n = 8) were subjected to aging at 2°C for 0, 7, 14, and 21 d. On each aging day, the muscles were fabricated into ISM and OSM steaks and allotted to refrigerated storage (2°C) under aerobic packaging. Instrumental color and metmyoglobin reducing activity were evaluated on d 0, 3, and 6 of storage. Samples frozen on d 0 and d 21 of aging were utilized for sarcoplasmic proteome analysis. Color attributes of both ISM and OSM steaks were influenced by aging, with steaks aged for 21 d having the lowest (P < 0.05) color stability. The ISM steaks had greater (P < 0.05) lightness than OSM counterparts, and the difference in lightness was not negated by aging. The ISM and OSM had similar (P > 0.05) redness on d 0 of storage, whereas ISM had lower (P < 0.05) redness compared to OSM on d 3 and d 6 of storage. Several proteins associated with glycolysis and energy metabolism were of greater abundance (P < 0.05) in OSM than in ISM after 21-d aging. Furthermore, the influence of 21-d aging on sarcoplasmic proteome was observed at a greater extent in OSM than in ISM, indicating that the effect of aging on sarcoplasmic proteome of beef semimembranosus was influenced by the location within the muscle.

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“…8 Recently, several articles have indicated the various effects of creatine including cytoprotective features through antioxidant-like effect, mitochondrial genome stabilization and decrease in oxygen consumption besides its ergogenic role. 9 Pioglitazone is an anti-diabetic drug that belongs to thiazolidinediones and acts as an agonist of peroxisome proliferator activated receptor gamma (PPARg). Along with its anti-inflammatory and antioxidant effects, pioglitazone has been reported to have protective effects on cerebral, myocardial and renal ischemia.…”

Section: Introductionmentioning

confidence: 99%

Effect of creatine and pioglitazone on Hk-2 cell line cisplatin nephrotoxicity

et al. 2014

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Cisplatin is a chemotherapeutic agent, which is used in the treatment of various solid organ cancers, and its main dose limiting side effect of cisplatin is nephrotoxicity. The aim of this study is to investigate the role of pioglitazone and creatine on cisplatin nephrotoxicity in vitro. Real-time cell analyzer system (RTCA) was used for real-time and time-dependent analysis of the cellular response of HK-2 cells following incubation with cisplatin and combination with creatine or pioglitazone hydrochloride. First, half-maximal inhibitory concentrations (IC50) of cisplatin, creatine and pioglitazone were calculated by RTCA system. Afterwards creatine and pioglitazone was administered with serial dilutions under RTCA system. IC50 dose for cisplatin was 7.69 M Â 10 À5 at 24th hour and 3.93 M Â 10 À6 at 48th hour. IC50 dose for pioglitazone was 1.61 M Â 10 À3 at 24th hour and 2.85 M Â 10 À4 at 48th hour. Although cells were treated the dose of 40,225 mM creatine, IC50 dose could not been reached. Neither pioglitazone nor creatine had additional protective effect in any dose. Consequently, beneficial effect of creatine and pioglitazone on cisplatin-induced cell death could not be found. Further studies and clinical trials are needed to evaluate the effect of different doses of these drugs in cisplatininduced nephrotoxicity.

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Creatine as an antioxidant

Cited by 166 publications

References 88 publications

Changes in the Sarcoplasmic Proteome of Beef Muscles with Differential Color Stability during Postmortem Aging

Changes in the Sarcoplasmic Proteome of Beef Muscles with Differential Color Stability during Postmortem Aging

Intramuscular Variations in Color and Sarcoplasmic Proteome of Beef Semimembranosus during Postmortem Aging

Effect of creatine and pioglitazone on Hk-2 cell line cisplatin nephrotoxicity

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