Creatine kinase activity and blood pressure in a normal population: the Tromsø study

Johnsen, Stein Harald; Lilleng, Hallvard; Wilsgaard, Tom; Bekkelund, Svein Ivar

doi:10.1097/hjh.0b013e32834068e0

Cited by 32 publications

(55 citation statements)

References 28 publications

(35 reference statements)

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“…This is in line with previous findings of CK as a main denominator of blood pressure 6,17,18 and reports of significant vasodilation of isolated resistance arteries after CK inhibition. 19 Furthermore, our data were collected in subjects of African and European ancestry and among the clinical spectrum of normotension to hypertension.…”

Section: Discussionsupporting

confidence: 93%

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Resistance Artery Creatine Kinase mRNA and Blood Pressure in Humans

et al. 2014

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Protocols were in accord with institutional guidelines and were approved by the local institutional review board. All participants gave written informed consent. Consecutive, self-defined white and African-Dutch women undergoing an abdominal procedure for fibroid enucleation or hysterectomy for fibroids were eligible for inclusion.Abstract-Hypertension remains the main risk factor for cardiovascular death. Environmental and biological factors are known to contribute to the condition, and circulating creatine kinase was reported to be the main predictor of blood pressure in the general population. This was proposed to be because of high resistance artery creatine kinase-BB rapidly regenerating ATP for vascular contractility. Therefore, we assessed whether creatine kinase isoenzyme mRNA levels in human resistance arteries are associated with blood pressure. We isolated resistance-sized arteries from omental fat donated by consecutive women undergoing uterine fibroid surgery. Blood pressure was measured in the sitting position. Vessels of 13 women were included, 6 normotensive and 7 hypertensive, mean age 42.9 years (SE, 1.6) and mean systolic/ diastolic blood pressure, 144.8 (8.0)/86.5 (4.3) mm Hg. Arteriolar creatine kinase isoenzyme mRNA was assessed using quantitative real-time polymerase chain reaction. Normalized creatine kinase B mRNA copy numbers, ranging from 5.2 to 24.4 (mean, 15.0; SE, 1.9), showed a near-perfect correlation with diastolic blood pressure (correlation coefficient, 0.9; 95% confidence interval, 0.6-1.0) and were well correlated with systolic blood pressure, with a 90% relative increase in resistance artery creatine kinase B mRNA in hypertensives compared with normotensives, normalized copy numbers were, respectively, 19.3 (SE, 2.0) versus 10.1 (SE, 2.1), P=0.0045. To our knowledge, this is the first direct evidence suggesting that resistance artery creatine kinase mRNA expression levels concur with blood pressure levels, almost doubling with hypertension. These findings add to the evidence that creatine kinase might be involved in the vasculature's pressor responses. (Hypertension. 2014;63:68-73.)

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Section: Discussionsupporting

confidence: 93%

“…17,18 Furthermore, we reported that human isolated resistance artery contractility depends on CK and that specific CK inhibitors greatly attenuate human vascular contractility in vitro. 19 The explanation proposed for these findings was that in the absence of organ damage, high serum CK activity reflected high tissue CK activity.…”

Section: Discussionmentioning

confidence: 99%

Resistance Artery Creatine Kinase mRNA and Blood Pressure in Humans

et al. 2014

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“…However, in that study, creatine kinase was not controlled for exercise, causing highly skewed data and a dilution of the association between creatine kinase and blood pressure [14,28]. Moreover, this study included only white individuals, and this population subgroup is known to have relatively low creatine and blood pressure levels, resulting in relatively small differences in blood pressure between treated controlled and treated uncontrolled patients.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, this study included only white individuals, and this population subgroup is known to have relatively low creatine and blood pressure levels, resulting in relatively small differences in blood pressure between treated controlled and treated uncontrolled patients. Because of these differences, this post-hoc analysis was underpowered [14,28]. In our paper, we analyzed differences in resting creatine kinase between all blood pressure categories, as a primary outcome, with adequate sample size, and also adjusted for other predictors, including sex, age, glucose, ethnicity, and BMI, resulting in a clear association between creatine kinase and treatment failure.…”

Section: Discussionmentioning

confidence: 99%

Creatine kinase is associated with failure of hypertension treatment

Oudman¹,

Kewalbansingh²,

Valkengoed³

et al. 2013

Journal of Hypertension

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“…Non-fasting blood samples were analysed by standard methods at the Department of Laboratory Medicine, University Hospital of North Norway. Details on data collection have been described elsewhere for Tromsø 2, 3 and 4,13 Tromsø 514 and Tromsø 6 15. Because of summer vacation, no measurements were performed in July.…”

Section: Methodsmentioning

confidence: 99%