Creatinine inhibits bacterial replication

McDonald, Tom; Drescher, Kristen M.; Weber, Annika; Tracy, Steven

doi:10.1038/ja.2011.131

Cited by 24 publications

(14 citation statements)

References 16 publications

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“…Out of the 18,065 unique genes inferred by Roary, 405 belonged to this strain. Among these genes, we identified creatinine amidohydrolase crnA (EI613_12750), an enzyme involved in the degradation of creatinine [30], which has been reported as replication-impairing molecule in bacteria [31]. The gene 3-alpha-hydroxysteroid dehydrogenase hsdA (EI613_09215), involved in steroid catabolism, is also exclusive to this strain and might assist Azospirillum sp.…”

Section: Resultsmentioning

confidence: 99%

Characterization of cellular, biochemical and genomic features of the diazotrophic plant growth-promoting bacteriumAzospirillumsp. UENF-412522, a novel member of theAzospirillumgenus

Rodrigues

Matteoli

Gazara

et al. 2021

Preprint

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Given their remarkable beneficial effects on plant growth, several Azospirillum isolates currently integrate the formulations of various commercial inoculants. Our research group isolated a new strain, Azospirillum sp. UENF-412522, from passion fruit rhizoplane. This isolate uses carbon sources that are partially distinct from closely-related Azospirillum isolates. Scanning electron microscopy analysis and population counts demonstrate the ability of Azospirillum sp. UENF-412522 to colonize the surface of passion fruit roots. In vitro assays demonstrate the ability of Azospirillum sp. UENF-412522 to fix atmospheric nitrogen, to solubilize phosphate and to produce indole-acetic acid. Passion fruit plantlets inoculated with Azospirillum sp. UENF-41255 showed increased shoot and root fresh matter, as well as root dry matter, further highlighting its biotechnological potential for agriculture. We sequenced the genome of Azospirillum sp. UENF-412522 to investigate the genetic basis of its plant-growth promotion properties. We identified the key nif genes for nitrogen fixation, the complete PQQ operon for phosphate solubilization, the acdS gene that alleviates ethylene effects on plant growth, and the napCAB operon, which produces nitrite under anoxic conditions. We also found several genes conferring resistance to common soil antibiotics, which are critical for Azospirillum sp. UENF-412522 survival in the rhizosphere. Finally, we also assessed the Azospirillum pangenome and highlighted key genes involved in plant growth promotion. A phylogenetic reconstruction of the genus was also conducted. Our results support Azospirillum sp. UENF-412522 as a good candidate for bioinoculant formulations focused on plant growth promotion in sustainable systems.

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Section: Resultsmentioning

confidence: 99%

Characterization of cellular, biochemical and genomic features of the diazotrophic plant growth-promoting bacteriumAzospirillumsp. UENF-412522, a novel member of theAzospirillumgenus

Rodrigues

Matteoli

Gazara

et al. 2021

Preprint

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“…In fact there is a lot of difference between the results and rarely have they been verified outside the laboratories. All this is likely related to the differences between laboratory conditions and real race conditions (only some sports include short exercises, repeated at intervals and requiring maximum strain), the existence of individuals who do not respond to treatment and although the literature is very wide, there are few serious studies, independent and reliable [3][4][5].…”

Section: Effects On Sport Performancementioning

confidence: 99%

Indications and Limitations of Creatinine in the Diet of Athletes

Ikonomi¹,

Anesti

2020

RISM

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“…Ganciclovir is generally associated with a variety of serious drugs. Common drug reactions (≥ 1% of patients) include granulocytopenia (Breedveld et al, 2017), neutropenia (Donadieu et al, 2017), anaemia (Stein et al, 2016), thrombocytopenia (Ahmed et al, 2007), fever, nausea, vomiting, dyspepsia (Duncanson et al, 2018), diarrhoea, abdominal pain, latulence (Bailey et al, 2009), anorexia (Espie and Eisler, 2015), increased liver enzymes, headache, confusion, hallucination (Chaudhury et al, 2009), seizures (Greenhalgh et al, 2020), pain and phlebitis (Nisio et al, 2015) at the injection site (due to high pH), sweating, rash, itching, increased levels of serum creatinine (McDonald et al, 2012) and blood urea.…”

Section: Introductionmentioning

confidence: 99%

New Method for the Estimation and Validation of Fostemsavir and Ganciclovir by using RP-HPLC

Surabhi

Jain

2021

ijrps

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We have evolved a completely unique, reliable HPLC technique for simultaneous quantification of Fostemsavir and Ganciclovir. The chromatographic detachment was attained on an X-Bridge phenyl column (150x4.6mm, 3.5 µ) using isocratic elution with a buffer containing 0.1% OPA and acetonitrile proportion of 50:50 as movable part with a stream of 1 mL/min at room temperature. The maximum absorbance of the drugs was observed at 236 nm. Dissolve 1mL of orthophosphoric acid in 1 lt of HPLC marked water and sieved by using 0.45 µ filter paper, this solution was used as a buffer. 10 min run time was used to separate Fostemsavir and Ganciclovir. Analysis was achieved within 15 min over honest linearity within the concentration range from 6-90 µg/mL of Fostemsavir and 2.5-37.5 µg/mL of Ganciclovir. To check the system suitability parameters, the normal solution was injected six times, from the outcomes, it was concluded that all the outcomes were well under the acceptable range. Precision and recovery study results were well under a suitable range. From this technique, an assay of Fostemsavir and Ganciclovir was performed and the results were well under the acceptable range. Degradation studies were carried out on Fostemsavir and Ganciclovir, with a purity threshold greater than the purity angle in all conditions and within the acceptable range. The above-mentioned technique was validated according to ICH guidelines.

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Creatinine inhibits bacterial replication

Cited by 24 publications

References 16 publications

Characterization of cellular, biochemical and genomic features of the diazotrophic plant growth-promoting bacteriumAzospirillumsp. UENF-412522, a novel member of theAzospirillumgenus

Characterization of cellular, biochemical and genomic features of the diazotrophic plant growth-promoting bacteriumAzospirillumsp. UENF-412522, a novel member of theAzospirillumgenus

Indications and Limitations of Creatinine in the Diet of Athletes

New Method for the Estimation and Validation of Fostemsavir and Ganciclovir by using RP-HPLC

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