Creation and grading of experimental corneal scars in mice models

Cogswell, Devon; Sun, Mei; Greenberg, Erin; Margo, Curtis E.; Espana, Edgar M.

doi:10.1016/j.jtos.2020.11.008

Cited by 12 publications

(14 citation statements)

References 29 publications

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“…CD31 is known as a member of the immunoglobulin superfamily. It mediates cell adhesion through endothelial cell expression [ 44 ]. Therefore, it is widely used in evaluating revascularization in the body.…”

Section: Resultsmentioning

confidence: 99%

Local bone metabolism balance regulation via double-adhesive hydrogel for fixing orthopedic implants

Jiang

Hou

et al. 2022

Bioactive Materials

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Section: Resultsmentioning

confidence: 99%

Local bone metabolism balance regulation via double-adhesive hydrogel for fixing orthopedic implants

Jiang

Hou

et al. 2022

Bioactive Materials

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“…Various groups across the globe have attempted to understand the basic biology and the mechanisms involved in the healing or repair of the corneal wounds resulting from trauma [ 49 , 50 , 51 ] or the regeneration of the corneal epithelium and stroma lost due to the regular wear and tear [ 30 , 52 ]. The epithelial homeostasis is achieved primarily through the LESCs residing in the limbal crypts [ 53 ], in which the systematic synthesis and the degradation of the collagens in the stromal extracellular matrix (ECM) released by the native keratocytes helps in the maintenance of the corneal stromal integrity and homeostasis [ 54 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Profiling of Human Limbus-Derived Stromal/Mesenchymal Stem Cells—Novel Mechanistic Insights into the Pathways Involved in Corneal Wound Healing

Tavakkoli

Damala

Koduri

et al. 2022

IJMS

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Limbus-derived stromal/mesenchymal stem cells (LMSCs) are vital for corneal homeostasis and wound healing. However, despite multiple pre-clinical and clinical studies reporting the potency of LMSCs in avoiding inflammation and scarring during corneal wound healing, the molecular basis for the ability of LMSCs remains unknown. This study aimed to uncover the factors and pathways involved in LMSC-mediated corneal wound healing by employing RNA-Sequencing (RNA-Seq) in human LMSCs for the first time. We characterized the cultured LMSCs at the stages of initiation (LMSC−P0) and pure population (LMSC−P3) and subjected them to RNA-Seq to identify the differentially expressed genes (DEGs) in comparison to native limbus and cornea, and scleral tissues. Of the 28,000 genes detected, 7800 DEGs were subjected to pathway-specific enrichment Gene Ontology (GO) analysis. These DEGs were involved in Wnt, TGF-β signaling pathways, and 16 other biological processes, including apoptosis, cell motility, tissue remodeling, and stem cell maintenance, etc. Two hundred fifty-four genes were related to wound healing pathways. COL5A1 (11.81 ± 0.48) and TIMP1 (20.44 ± 0.94) genes were exclusively up-regulated in LMSC−P3. Our findings provide new insights involved in LMSC-mediated corneal wound healing.

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“…Opacities were graded from 1 to 3 for severity and extent based on a modified Fantes haze scale (see Table 2 ; Fig. 2 ) [ 21 ]. The severity of corneal vascularization was classified by range involved and vessel density.…”

Section: Methodsmentioning

confidence: 99%

Classifications of anterior segment structure of congenital corneal opacity in infants and toddlers by ultrasound biomicroscopy and slit-lamp microscopic photographs: an observational study

Hong,

Xie,

Wang

et al. 2024

BMC Ophthalmol

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Background The structural features have an impact on the surgical prognosis for congenital corneal opacity (CCO). The structural classification system of CCO, however, is lacking. Based on data from ultrasound biomicroscopy (UBM) findings in infants and toddlers with CCO, this research proposed a classification system for the anterior segment structure severity. Methods Medical records, preoperative UBM images and slit-lamp photographs of infants and toddlers diagnosed with CCO at University Third Hospital between December 2018 and June 2022 were reviewed. According to the anterior segment structural features observed in UBM images, eyes were classified as follows: U1, opaque cornea only; U2, central anterior synechia; U3, peripheral anterior synechia combined with angle closure; and U4, aniridia or lens anomaly. The opacity appearance and corneal vascularization density observed in slit-lamp photographs were assigned grades according to previous studies. The extent of vascularization was also recorded. The corresponding intraocular anomaly classifications and ocular surface lesion severity were analysed. Results Among 81 eyes (65 patients), 41 (50.6%) were right eyes, and 40 (49.4%) were left eyes. The median age at examination was 6.91 months (n = 81, 1.00, 34.00). Two (2.5%) of the 81 eyes were classified as U1, 20 (24.7%) as U2, 22 (27.2%) as U3a, 11 (13.6%) as U3b and 26 (32.1%) as U4. Bilateral CCO eyes had more severe UBM classifications (P = 0.019), more severe dysgenesis (P = 0.012) and a larger angle closure (P = 0.009). Eyes with more severe UBM classifications had higher opacity grades (P = 0.003) and vascularization grades (P = 0.014) and a larger vascularization extent (P = 0.001). Eyes with dysgenesis had higher haze grades (P = 0.012) and more severe vascularization (P = 0.003 for density; P = 0.008 for extent), while the angle closure range was related to haze grade (P = 0.013) and vascularization extent (P = 0.003). Conclusions This classification method based on UBM and slit-lamp photography findings in the eyes of CCO infants and toddlers can truly reflect the degree of abnormality of the ocular surface and anterior segment and is correlated with the severity of ocular surface anomalies. This method might provide meaningful guidance for surgical procedure design and prognostic determinations for keratoplasty in CCO eyes.

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Creation and grading of experimental corneal scars in mice models

Cited by 12 publications

References 29 publications

Local bone metabolism balance regulation via double-adhesive hydrogel for fixing orthopedic implants

Local bone metabolism balance regulation via double-adhesive hydrogel for fixing orthopedic implants

Transcriptomic Profiling of Human Limbus-Derived Stromal/Mesenchymal Stem Cells—Novel Mechanistic Insights into the Pathways Involved in Corneal Wound Healing

Classifications of anterior segment structure of congenital corneal opacity in infants and toddlers by ultrasound biomicroscopy and slit-lamp microscopic photographs: an observational study

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