Creation of bladder assembloids mimicking tissue regeneration and cancer

Kim, Eunjee; Choi, Seong Soo; Kang, Byung Ha; Kong, Jung Ho; Kim, Dae Young; Yoon, Woong; Lee, Hwa Rim; Kim, Sung Eun; Kim, Hyo Min; Lee, Hye Sun; Yang, Chorong; Lee, You Jeong; Kang, Minyong; Roh, Tae-Young; Jung, Sungjune; Kim, Sanguk; Ku, Ja Hyeon; Shin, Kunyoo

doi:10.1038/s41586-020-3034-x

Cited by 173 publications

(135 citation statements)

References 74 publications

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“… 77 To overcome these limitations of organoids, a multilayer bladder called an “assembloids” has been created by reconstituting tissue stem cells with stromal components representative of an organized architecture. 78 In sum, either classical 2D/3D cultures or assembloids will benefit functional studies of CAFs in the context of the gradually accepted importance of TME.…”

Section: From Fibroblasts To Cafs In Tmementioning

confidence: 99%

Signaling pathways in cancer-associated fibroblasts and targeted therapy for cancer

Yang

Liu

et al. 2021

Sig Transduct Target Ther

369

249

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To flourish, cancers greatly depend on their surrounding tumor microenvironment (TME), and cancer-associated fibroblasts (CAFs) in TME are critical for cancer occurrence and progression because of their versatile roles in extracellular matrix remodeling, maintenance of stemness, blood vessel formation, modulation of tumor metabolism, immune response, and promotion of cancer cell proliferation, migration, invasion, and therapeutic resistance. CAFs are highly heterogeneous stromal cells and their crosstalk with cancer cells is mediated by a complex and intricate signaling network consisting of transforming growth factor-beta, phosphoinositide 3-kinase/AKT/mammalian target of rapamycin, mitogen-activated protein kinase, Wnt, Janus kinase/signal transducers and activators of transcription, epidermal growth factor receptor, Hippo, and nuclear factor kappa-light-chain-enhancer of activated B cells, etc., signaling pathways. These signals in CAFs exhibit their own special characteristics during the cancer progression and have the potential to be targeted for anticancer therapy. Therefore, a comprehensive understanding of these signaling cascades in interactions between cancer cells and CAFs is necessary to fully realize the pivotal roles of CAFs in cancers. Herein, in this review, we will summarize the enormous amounts of findings on the signals mediating crosstalk of CAFs with cancer cells and its related targets or trials. Further, we hypothesize three potential targeting strategies, including, namely, epithelial–mesenchymal common targets, sequential target perturbation, and crosstalk-directed signaling targets, paving the way for CAF-directed or host cell-directed antitumor therapy.

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Section: From Fibroblasts To Cafs In Tmementioning

confidence: 99%

Signaling pathways in cancer-associated fibroblasts and targeted therapy for cancer

Yang

Liu

et al. 2021

Sig Transduct Target Ther

369

249

View full text Add to dashboard Cite

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“…Some of them were even patented (Cross, 2003). Recently, Kim et al reported the most complex 3D bladder multilayered model, mimicking an organized architecture of a mature organ through the assembly of a urothelium surrounding stroma and an outer muscle layer (Kim et al, 2020). Interestingly, apart from the 3D organoid described by Horsley et al in 2018, all the aforementioned in vitro models were not exposed to urine, or if so, only for a few hours, up to a dayalthough a few studies report the use of urine components (e.g.…”

Section: In Vitro Human Cell-based Modelsmentioning

confidence: 99%

Section: In Vitro Human Cell-based Modelsmentioning

confidence: 99%

Recurrent Urinary Tract Infection: A Mystery in Search of Better Model Systems

Murray

Flores

Williams

et al. 2021

Front. Cell. Infect. Microbiol.

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Urinary tract infections (UTIs) are among the most common infectious diseases worldwide but are significantly understudied. Uropathogenic E. coli (UPEC) accounts for a significant proportion of UTI, but a large number of other species can infect the urinary tract, each of which will have unique host-pathogen interactions with the bladder environment. Given the substantial economic burden of UTI and its increasing antibiotic resistance, there is an urgent need to better understand UTI pathophysiology – especially its tendency to relapse and recur. Most models developed to date use murine infection; few human-relevant models exist. Of these, the majority of in vitro UTI models have utilized cells in static culture, but UTI needs to be studied in the context of the unique aspects of the bladder’s biophysical environment (e.g., tissue architecture, urine, fluid flow, and stretch). In this review, we summarize the complexities of recurrent UTI, critically assess current infection models and discuss potential improvements. More advanced human cell-based in vitro models have the potential to enable a better understanding of the etiology of UTI disease and to provide a complementary platform alongside animals for drug screening and the search for better treatments.

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“…Therefore, signal transduction pathway regulation may vary depending on cancer types and tissue origins (Schneider et al, 2017). Recently, bioprinted in vitro cancer models have been demonstrated to closely simulate the properties of actual cancers, including their defining phenotypes and pathological characteristics and distinct tissue-specific TME heterogeneity (Yi et al, 2019;Kim E. et al, 2020;Yoon et al, 2020). Heinrich et al (2019) developed a glioblastoma (GBM) model based on a mini-brain construct containing macrophages (Figure 7A).…”

Section: D Bioprinting Approaches For the Recapitulation Of Tumor Microenvironmentsmentioning

confidence: 99%

3D Bioprinting-Based Vascularized Tissue Models Mimicking Tissue-Specific Architecture and Pathophysiology for in vitro Studies

Hwang

Choi

Jang

2021

Front. Bioeng. Biotechnol.

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A wide variety of experimental models including 2D cell cultures, model organisms, and 3D in vitro models have been developed to understand pathophysiological phenomena and assess the safety and efficacy of potential therapeutics. In this sense, 3D in vitro models are an intermediate between 2D cell cultures and animal models, as they adequately reproduce 3D microenvironments and human physiology while also being controllable and reproducible. Particularly, recent advances in 3D in vitro biomimicry models, which can produce complex cell structures, shapes, and arrangements, can more similarly reflect in vivo conditions than 2D cell culture. Based on this, 3D bioprinting technology, which enables to place the desired materials in the desired locations, has been introduced to fabricate tissue models with high structural similarity to the native tissues. Therefore, this review discusses the recent developments in this field and the key features of various types of 3D-bioprinted tissues, particularly those associated with blood vessels or highly vascularized organs, such as the heart, liver, and kidney. Moreover, this review also summarizes the current state of the three categories: (1) chemical substance treatment, (2) 3D bioprinting of lesions, and (3) recapitulation of tumor microenvironments (TME) of 3D bioprinting-based disease models according to their disease modeling approach. Finally, we propose the future directions of 3D bioprinting approaches for the creation of more advanced in vitro biomimetic 3D tissues, as well as the translation of 3D bioprinted tissue models to clinical applications.

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Creation of bladder assembloids mimicking tissue regeneration and cancer

Cited by 173 publications

References 74 publications

Signaling pathways in cancer-associated fibroblasts and targeted therapy for cancer

Signaling pathways in cancer-associated fibroblasts and targeted therapy for cancer

Recurrent Urinary Tract Infection: A Mystery in Search of Better Model Systems

3D Bioprinting-Based Vascularized Tissue Models Mimicking Tissue-Specific Architecture and Pathophysiology for in vitro Studies

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