2008
DOI: 10.1002/0471142735.im1521s81
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Creation of “Humanized” Mice to Study Human Immunity

Abstract: Abstract"Humanized" mice are a promising translational model for studying human hematopoiesis and immunity. Their utility has been enhanced by the development of new stocks of immunodeficient hosts, most notably mouse strains such as NOD-scid IL2rγ null mice that lack the IL-2 receptor common gamma chain. These stocks of mice lack adaptive immune function, display multiple defects in innate immunity, and support heightened levels of human hematolymphoid engraftment. Humanized mice can support studies in many a… Show more

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Cited by 182 publications
(205 citation statements)
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“…A complete description of the construction of humanized mice used in this study is published elsewhere (43,44). Briefly, newborn NOD-scid Typhi were grown in TB with kanamycin and diluted into PBS to desired concentrations for inoculation with cfu as indicated.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…A complete description of the construction of humanized mice used in this study is published elsewhere (43,44). Briefly, newborn NOD-scid Typhi were grown in TB with kanamycin and diluted into PBS to desired concentrations for inoculation with cfu as indicated.…”
Section: Methodsmentioning
confidence: 99%
“…The IL-2r γ-chain is required not only for IL-2 high-affinity ligand binding and intracellular signaling but also for IL-4, IL-7, IL-9, IL-15, and IL-21 binding and signaling (42), and its absence results in a complete block of mature T cell, B cell, and NK (natural killer) cell development. Sublethally irradiated newborn NOD-scid IL2rγ null mice engrafted with CD34 + hematopoietic stem cells (HSC) from T cell-depleted human umbilical cord blood (hu-SRC-SCID or human-SCID repopulating cell-SCID mice) develop into mice with chimeric hematopoietic systems containing human immune cells in the immunodeficient mouse environment (42), including human B cells, CD4 + and CD8+ T cells, NK cells, monocytes, and myeloid and plasmacytoid dendritic cells (43,44).…”
mentioning
confidence: 99%
“…Lacking mature B and T cells, NOD.scid mice are both insulitisand diabetes-free throughout life. However, because of a high incidence of thymic lymphomas, the mean lifespan is relatively short [13,23,24] . Accordingly, while the unique immune defects provide an excellent in vivo environment for hematopoietic investigation extending to effects within the marrow compartment, this model may not be suitable for assessing bone material properties.…”
mentioning
confidence: 99%
“…However, ideally we would like to study the disease directly in humans, as the outcomes predicted by murine studies do not match the outcomes of clinical trials. This especially applies in case of clinical trials for T1D prevention and treatment [78]. Using three transcription factors (Oct4, Sox2, and KLF4), T1D patient-specific pluripotent cell lines have been generated [64,79], termed as DiPS.…”
Section: Alternative Pathways To β-Cells: Reprogramming Of Mature Cellsmentioning
confidence: 99%
“…T cells are educated on thymic epithelium such that they can distinguish between self and foreign molecules. With all these cells available, the disease could be recreated either in vitro or in vivo using humanized mouse models [78]. All these studies might be instrumental in the generation of β-cells resistant to autoimmune attack.…”
Section: Alternative Pathways To β-Cells: Reprogramming Of Mature Cellsmentioning
confidence: 99%