CRISPR / Cas 9 assisted genome editing technology for the improvement of Horticultural crops

Muthu, Valarmathi; JAYAKUMAR, SOWMIYA

doi:10.31254/phyto.2023.12110

Cited by 2 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Homing meganucleases, zinc finger nucleases, transcription activator-like effector nucleases, and CRISPR-Cas9 and -Cas12a orthologs are the four types of designed nucleases created for genome engineering. The first three approaches, however, have a closed architecture that makes manipulating target specificity difficult, time-consuming, and expensive ( Guha & Edgell, 2017 ; Chandran et al., 2023 ).…”

Section: Crop Improvement Using Crispr-cas As Genetic Engineering Toolmentioning

confidence: 99%

Adoption of CRISPR-Cas for crop production: present status and future prospects

Akanmu,

Asemoloye,

Marchisio

et al. 2024

PeerJ

View full text Add to dashboard Cite

Background Global food systems in recent years have been impacted by some harsh environmental challenges and excessive anthropogenic activities. The increasing levels of both biotic and abiotic stressors have led to a decline in food production, safety, and quality. This has also contributed to a low crop production rate and difficulty in meeting the requirements of the ever-growing population. Several biotic stresses have developed above natural resistance in crops coupled with alarming contamination rates. In particular, the multiple antibiotic resistance in bacteria and some other plant pathogens has been a hot topic over recent years since the food system is often exposed to contamination at each of the farm-to-fork stages. Therefore, a system that prioritizes the safety, quality, and availability of foods is needed to meet the health and dietary preferences of everyone at every time. Methods This review collected scattered information on food systems and proposes methods for plant disease management. Multiple databases were searched for relevant specialized literature in the field. Particular attention was placed on the genetic methods with special interest in the potentials of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and Cas (CRISPR associated) proteins technology in food systems and security. Results The review reveals the approaches that have been developed to salvage the problem of food insecurity in an attempt to achieve sustainable agriculture. On crop plants, some systems tend towards either enhancing the systemic resistance or engineering resistant varieties against known pathogens. The CRISPR-Cas technology has become a popular tool for engineering desired genes in living organisms. This review discusses its impact and why it should be considered in the sustainable management, availability, and quality of food systems. Some important roles of CRISPR-Cas have been established concerning conventional and earlier genome editing methods for simultaneous modification of different agronomic traits in crops. Conclusion Despite the controversies over the safety of the CRISPR-Cas system, its importance has been evident in the engineering of disease- and drought-resistant crop varieties, the improvement of crop yield, and enhancement of food quality.

show abstract

Section: Crop Improvement Using Crispr-cas As Genetic Engineering Toolmentioning

confidence: 99%

Adoption of CRISPR-Cas for crop production: present status and future prospects

Akanmu,

Asemoloye,

Marchisio

et al. 2024

PeerJ

View full text Add to dashboard Cite

show abstract

A Review: CRISPR Cas System and the Mechanism With an Inhibition of Binding of CRISPR Cas‐9

Hosen,

Nishat,

Soaib

et al. 2024

Nano Select

View full text Add to dashboard Cite

CRISPR Cas system is a revolutionary and precise genome editing system that induces bacterial and archaeal adaptive immunity and already has shown several promising applications in the treatment of genetic diseases and detection of nucleic acid targets. It is a complex tool, comprising diverse effector proteins and guide RNA that can be applied to dissociate and modify genes in living organisms along with the in vitro process. The review concentrates on the advances accomplished from some recent years to 2023 for the emergence of predicting actions of Cas protein with the guide RNA (gRNA), playing a key role in genome editing. This review emphasizes the effect of DNA structure and chromosomal protein on Cas 9 binding with a distinct classification of Cas types. The relevance of this review is highlighted by the significance of the rapid development of wide study methods and their potential impacts on the efficiency of the CRISPR Cas system. We have analyzed recent kinds of literature in this work to integrate a deep learning way that contributes over the next time with some important discussion about viral resistance in eukaryotes along with some cancer treatment strategies, and crop improvement by the CRISPR Cas system.

show abstract

CRISPR / Cas 9 assisted genome editing technology for the improvement of Horticultural crops

Cited by 2 publications

References 0 publications

Adoption of CRISPR-Cas for crop production: present status and future prospects

Adoption of CRISPR-Cas for crop production: present status and future prospects

A Review: CRISPR Cas System and the Mechanism With an Inhibition of Binding of CRISPR Cas‐9

Contact Info

Product

Resources

About