CRISPR/Cas 9-Based Editing in the Production of Bioactive Molecules

Bhagwat, Amrita C.; Patil, Amrita M; Saroj, Sunil D.

doi:10.1007/s12033-021-00418-4

Cited by 17 publications

(6 citation statements)

References 43 publications

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“…It has been demonstrated that the CRISPR-Cas9 system is capable of successfully enhancing disease resistance in crops such as tomato, hence offering additional protection against pathogens such as powdery mildew and bacterial spot. Grapevines were subjected to CRISPR-Cas9, which allowed the researchers to target and modify the MLO (Mildew Resistance Locus O) gene [19]. This led to the production of powderymildew-resistant grape types.…”

Section: Fig 3 Cas9 Nuclease and The Guide Rnamentioning

confidence: 99%

Advancements in Genetic Engineering for Enhanced Traits in Horticulture Crops: A Comprehensive Review

Bhavanee,

Krishnamoorthi,

Rathva

et al. 2024

JABB

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Genetic engineering has emerged as a powerful tool in the field of horticulture to enhance the traits of crops, leading to improved yield, quality, and resistance to biotic and abiotic stresses. This comprehensive review explores the recent advancements in genetic engineering techniques applied to horticulture crops, providing a detailed overview of the innovative strategies employed to manipulate plant genomes. The review begins by discussing the evolution of genetic engineering in horticulture, highlighting key milestones and breakthroughs that have paved the way for current advancements. It covers a range of techniques such as CRISPR-Cas9, RNA interference, and synthetic biology, shedding light on their applications in modifying specific genes responsible for desired traits. The main focus of the review is on the enhancement of key horticultural traits, including disease resistance, pest resistance, abiotic stress tolerance, and post-harvest attributes. Examples from various horticultural crops, such as fruits, vegetables, and ornamental plants, illustrate the success stories and potential applications of genetic engineering in each category. The ethical and regulatory aspects of genetic engineering in horticulture are also explored, addressing concerns related to environmental impact, biodiversity, and consumer acceptance. The review emphasizes the importance of responsible and sustainable practices in the application of genetic engineering to ensure the long-term benefits without adverse consequences. Furthermore, the review delves into emerging trends and future prospects in the field, including the potential of genome editing for precision breeding, the use of omics technologies for targeted trait improvements, and the integration of genetic engineering with other breeding techniques. It also discusses the challenges and opportunities associated with the global adoption of genetically modified horticulture crops.

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Section: Fig 3 Cas9 Nuclease and The Guide Rnamentioning

confidence: 99%

Advancements in Genetic Engineering for Enhanced Traits in Horticulture Crops: A Comprehensive Review

Bhavanee,

Krishnamoorthi,

Rathva

et al. 2024

JABB

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“…A versatile and widely used technique for precise DNA editing Improve yield, disease resistance, quality traits, and stress tolerance [19] TALEN Transcription activator-like effector nuclease for targeted editing Modify specific genes for desired traits [14] Zinc Finger Nuclease (ZFNs) Engineered DNA-binding proteins for targeted gene editing Enhance disease resistance, improve nutritional value, gene disruption, and gene replacement [20]…”

Section: Crispr-cas9mentioning

confidence: 99%

Enhancing Horticultural Crops through Genome Editing: Applications, Benefits, and Considerations

Daniel¹,

Raveendar

et al. 2023

Horticulturae

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Genome editing has emerged as a powerful tool for accelerating crop improvement in horticultural crops by enabling precise modifications to their genetic makeup. This review provides an in-depth exploration of the applications, methodologies, and potential impacts of genome editing in horticulture. The review focuses on three major genome editing tools in horticulture, CRISPR-Cas9, TALENs, and ZFNs. The underlying mechanisms, applications, and potential challenges associated with each tool are discussed in detail. CRISPR-Cas9, being a versatile and widely used system, has the potential to enhance traits such as disease resistance, abiotic stress tolerance, nutritional content, and yield in horticultural crops. TALENs and ZFNs, although less commonly used, offer alternative options for targeted DNA modifications, and have demonstrated success in specific applications. We emphasize the potential benefits of genome editing in horticulture, including improved crop productivity, quality, and nutritional value. However, challenges such as off-target effects, delivery methods, and regulatory frameworks need to be addressed for the full realization of this technology’s potential. This review serves as a valuable resource for researchers, policymakers, and stakeholders, providing insights into the opportunities and complexities associated with harnessing genome editing for enhanced traits in horticultural crops. By navigating these challenges, genome editing can contribute to sustainable advancements in horticulture, benefiting both producers and consumers worldwide.

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“…They are generated through the phenylpropanoid pathway. This bioactive has a lot of biological advantages, including reduced risk of cancer, osteoporosis, cardiovascular disease, and other diseases, as well as use as an antioxidant (Bhagwat et al, 2022). Notably, soybean has an isoflavonoid level that is almost 100 times higher than that of other legumes.…”

Section: Increase In Isoflavonoid Contentmentioning

confidence: 99%

CRISPR-Cas9 mediated genome editing in soybean for improving quality traits

Haider

2023

JINAGRI

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Legumes are the major source of energy for people throughout the world and play a significant role in a balanced diet to satisfy the body's need for protein. Soybean (Glycine max L.) is also a poor man meat which is a highly enriched amount of protein present in it. Day-by-day increase in the worldwide population is also a great challenge to improve the yield and nutritional values. Here are some exciting ways to improve the yield and nutrition values through basic and advanced techniques that are particularly important and worldwide use. A unique idea called "biofortification" involves the enrichment of micronutrients using traditional plant breeding and contemporary technologies. Research on grain bio-fortification has considerably reduced hunger globally over the past few decades. The current biofortification programs are now more competitive due to a better understanding of the food matrix. Recent advancements in biotechnology have a variety of positive effects, and genetic engineering is developing quickly. Since genome editing technology has made it possible to precisely alter and change the genomes of living beings, it has transformed genetic and biological research, the simplest example is CRISPR CAS9. We concentrate on the most recent developments in CRISPR/Cas9-based technology and talk about the 2 www.cornous.com prospects and difficulties of using this ground-breaking technology to improve specific characteristics in soybeans and other crops.

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CRISPR/Cas 9-Based Editing in the Production of Bioactive Molecules

Cited by 17 publications

References 43 publications

Advancements in Genetic Engineering for Enhanced Traits in Horticulture Crops: A Comprehensive Review

Advancements in Genetic Engineering for Enhanced Traits in Horticulture Crops: A Comprehensive Review

Enhancing Horticultural Crops through Genome Editing: Applications, Benefits, and Considerations

CRISPR-Cas9 mediated genome editing in soybean for improving quality traits

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