CRISPR/Cas- and Topical RNAi-Based Technologies for Crop Management and Improvement: Reviewing the Risk Assessment and Challenges Towards a More Sustainable Agriculture

Távora, Fabiano Touzdjian Pinheiro Kohlrausch; Diniz, Francisco de Assis dos Santos; Rêgo-Machado, Camila de Moraes; Freitas, Natália Chagas; Arraes, Fabrício Barbosa Monteiro; Andrade, E. C. de; Furtado, Leila Lourenço; Osiro, Karen Ofuji; Sousa, Natália Lima de; Cardoso, Thiago Bérgamo; Mertz-Henning, L. M.; Molinari, Patrícia Abrão de Oliveira; Feingold, Sérgio Enrique; Hunter, Wayne B.; Sá, Maria Fátima Grossi de; Kobayashi, Adilson Kenji; Nepomuceno, Alexandre Lima; Santiago, Thaís Ribeiro; Molinari, Hugo Bruno Correa

doi:10.3389/fbioe.2022.913728

Cited by 23 publications

(15 citation statements)

References 271 publications

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“…Recently, the use of modern, powerful and non-transgenic technologies like clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated genes (Cas) and RNAi has revolutionized different sectors of plant research ( Touzdjian Pinheiro Kohlrausch Távora et al., 2022 ). For instance, using RNAi to develop transgenic plants to combat geminiviruses and their insect vector have been proposed in the last decade ( Kumar and Sarin, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

Cotton leaf curl Multan virus differentially regulates innate antiviral immunity of whitefly (Bemisia tabaci) vector to promote cryptic species-dependent virus acquisition

Farooq

She

et al. 2022

Front. Plant Sci.

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Begomoviruses represent the largest group of economically important, highly pathogenic, DNA plant viruses that contribute a substantial amount of global crop disease burden. The exclusive transmission of begomoviruses by whiteflies (Bemisia tabaci) requires them to interact and efficiently manipulate host responses at physiological, biological and molecular scales. However, the molecular mechanisms underlying complex begomovirus-whitefly interactions that consequently substantiate efficient virus transmission largely remain unknown. Previously, we found that whitefly Asia II 7 cryptic species can efficiently transmit cotton leaf curl Multan virus (CLCuMuV) while MEAM1 cryptic species is a poor carrier and incompetent vector of CLCuMuV. To investigate the potential mechanism/s that facilitate the higher acquisition of CLCuMuV by its whitefly vector (Asia II 7) and to identify novel whitefly proteins that putatively interact with CLCuMuV-AV1 (coat protein), we employed yeast two-hybrid system, bioinformatics, bimolecular fluorescence complementation, RNA interference, RT-qPCR and bioassays. We identified a total of 21 Asia II 7 proteins putatively interacting with CLCuMuV-AV1. Further analyses by molecular docking, Y2H and BiFC experiments validated the interaction between a whitefly innate immunity-related protein (BTB/POZ) and viral AV1 (coat protein). Gene transcription analysis showed that the viral infection significantly suppressed the transcription of BTB/POZ and enhanced the accumulation of CLCuMuV in Asia II 7, but not in MEAM1 cryptic species. In contrast to MEAM1, the targeted knock-down of BTB/POZ substantially reduced the ability of Asia II 7 to acquire and accumulate CLCuMuV. Additionally, antiviral immune signaling pathways (Toll, Imd, Jnk and Jak/STAT) were significantly suppressed following viral infection of Asia II 7 whiteflies. Taken together, the begomovirus CLCuMuV potentiates efficient virus accumulation in its vector B. tabaci Asia II 7 by targeting and suppressing the transcription of an innate immunity-related BTB/POZ gene and other antiviral immune responses in a cryptic species-specific manner.

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Section: Discussionmentioning

confidence: 99%

Cotton leaf curl Multan virus differentially regulates innate antiviral immunity of whitefly (Bemisia tabaci) vector to promote cryptic species-dependent virus acquisition

Farooq

She

et al. 2022

Front. Plant Sci.

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“…Development of regulatory protocols for modern biotechniques such as RNAi and CRISPR is essential before intended deployment. However, the regulatory protocols can vary across jurisdictions, predominantly emphasizing the process or the end product due to associated risks and the need to effectively manage toxicity levels to non-target organisms (Ahmad et al 2021;Távora et al 2022 (Frewer et al 2013;Gupta et al 2015;Beghin and Gustafson 2021).…”

Section: Ethical and Legal Implications Of Rnai And Gene Editing Tech...mentioning

confidence: 99%

Understanding bark beetle outbreaks: exploring the impact of changing temperature regimes, droughts, forest structure, and prospects for future forest pest management

Singh,

Naseer,

Mogilicherla

et al. 2024

Rev Environ Sci Biotechnol

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Climate change has increased the susceptibility of forest ecosystems, resulting in escalated forest decline globally. As one of the largest forest biomasses in the Northern Hemisphere, the Eurasian boreal forests are subjected to frequent drought, windthrow, and high-temperature disturbances. Over the last century, bark beetle outbreaks have emerged as a major biotic threat to these forests, resulting in extensive tree mortality. Despite implementing various management strategies to mitigate the bark beetle populations and reduce tree mortality, none have been effective. Moreover, altered disturbance regimes due to changing climate have facilitated the success of bark beetle attacks with shorter and multivoltine life cycles, consequently inciting more frequent bark beetle-caused tree mortality. This review explores bark beetle population dynamics in the context of climate change, forest stand dynamics, and various forest management strategies. Additionally, it examines recent advancements like remote sensing and canine detection of infested trees and focuses on cutting-edge molecular approaches including RNAi-nanoparticle complexes, RNAi-symbiotic microbes, sterile insect technique, and CRISPR/Cas9-based methods. These diverse novel strategies have the potential to effectively address the challenges associated with managing bark beetles and improving forest health in response to the changing climate.

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“…But the poor stability of mRNA and reduced efficiency of gene editing with mRNA are the major limitations of this approach. Ribonucleoprotein (RNP) based CRISPR-Cas system has also been used for CRISPR applications in plants, which results in obtaining transgene-free plants, which are not considered GM according to one concept ( Távora et al., 2022 ). For example, Cas9 protein and sgRNA as mRNA were delivered through lipofection in plants to obtain transgene free CRISPR edited plants ( Zhang S. et al., 2021 ).…”

Section: Genetically Modified or Non-genetically Modified?mentioning

confidence: 99%

GMOs or non-GMOs? The CRISPR Conundrum

Ahmad,

Jamil,

Munawar

2023

Front. Plant Sci.

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CRISPR-Cas9, the “genetic scissors”, is being presaged as a revolutionary technology, having tremendous potential to create designer crops by introducing precise and targeted modifications in the genome to achieve global food security in the face of climate change and increasing population. Traditional genetic engineering relies on random and unpredictable insertion of isolated genes or foreign DNA elements into the plant genome. However, CRISPR-Cas based gene editing does not necessarily involve inserting a foreign DNA element into the plant genome from different species but introducing new traits by precisely altering the existing genes. CRISPR edited crops are touching markets, however, the world community is divided over whether these crops should be considered genetically modified (GM) or non-GM. Classification of CRISPR edited crops, especially transgene free crops as traditional GM crops, will significantly affect their future and public acceptance in some regions. Therefore, the future of the CRISPR edited crops is depending upon their regulation as GM or non-GMs, and their public perception. Here we briefly discuss how CRISPR edited crops are different from traditional genetically modified crops. In addition, we discuss different CRISPR reagents and their delivery tools to produce transgene-free CRISPR edited crops. Moreover, we also summarize the regulatory classification of CRISPR modifications and how different countries are regulating CRISPR edited crops. We summarize that the controversy of CRISPR-edited plants as GM or non-GM will continue until a universal, transparent, and scalable regulatory framework for CRISPR-edited plants will be introduced worldwide, with increased public awareness by involving all stakeholders.

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CRISPR/Cas- and Topical RNAi-Based Technologies for Crop Management and Improvement: Reviewing the Risk Assessment and Challenges Towards a More Sustainable Agriculture

Cited by 23 publications

References 271 publications

Cotton leaf curl Multan virus differentially regulates innate antiviral immunity of whitefly (Bemisia tabaci) vector to promote cryptic species-dependent virus acquisition

Cotton leaf curl Multan virus differentially regulates innate antiviral immunity of whitefly (Bemisia tabaci) vector to promote cryptic species-dependent virus acquisition

Understanding bark beetle outbreaks: exploring the impact of changing temperature regimes, droughts, forest structure, and prospects for future forest pest management

GMOs or non-GMOs? The CRISPR Conundrum

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