CRISPR/Cas technology promotes the various application of Dunaliella salina system

Feng, Shi Ting; Hu, Lina; Zhang, Qihang; Zhang, Fangqing; Du, Jingxia; Liang, Guozheng; Li, Aifang; Song, Guannan; Liu, Yu

doi:10.1007/s00253-020-10892-6

Cited by 22 publications

(13 citation statements)

References 114 publications

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“…salina . [ 190 ] However, target analysis character is still a limitation due to the complications in WWT using microalgae or microalgae‐bacterial consortia platform and following biorefinery application. These analyses require combined analytical and multiomics approaches to achieve a broad panorama.…”

Section: Potential Development Of Genetic Engineering Of Microalgae F...mentioning

confidence: 99%

Multiomics approaches and genetic engineering of metabolism for improved biorefinery and wastewater treatment in microalgae

Kuo

Yang

Chien

et al. 2022

Biotechnology Journal

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Microalgae, a group of photosynthetic microorganisms rich in diverse and novel bioactive metabolites, have been explored for the production of biofuels, high value-added compounds as food and feeds, and pharmaceutical chemicals as agents with therapeutic benefits. This article reviews the development of omics resources and genetic engineering techniques including gene transformation methodologies, mutagenesis, and genome-editing tools in microalgae biorefinery and wastewater treatment (WWT).The introduction of these enlisted techniques has simplified the understanding of complex metabolic pathways undergoing microalgal cells. The multiomics approach of the integrated omics datasets, big data analysis, and machine learning for the discovery of objective traits and genes responsible for metabolic pathways was reviewed. Recent advances and limitations of multiomics analysis and genetic bioengineering technology to facilitate the improvement of microalgae as the dual role of WWT and biorefinery feedstock production are discussed.

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Section: Potential Development Of Genetic Engineering Of Microalgae F...mentioning

confidence: 99%

Multiomics approaches and genetic engineering of metabolism for improved biorefinery and wastewater treatment in microalgae

Kuo

Yang

Chien

et al. 2022

Biotechnology Journal

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“…1). Using these constructed plasmids, D. salina cells were transformed separately with salt gradients method (Feng et al 2020). After 2-3 days transformation, cells were cultured in fresh medium with 700 µg/mL G418 in a 12-well plate at 26℃ light incubator.…”

Section: Vector Construction and D Salina Transformationmentioning

confidence: 99%

“…And now, CRISPR/ Cas system has been used as an enormously powerful Open Access *Correspondence: fshy001@haust.edu.cn † Lina Hu and Shuying Feng contributed equally to this work 1 School of Basic Medical Sciences, Henan University of Science and Technology, No. 263 Kaiyuan Avenue, Luoyang 471023, Henan, China Full list of author information is available at the end of the article tool in the drug discovery, disease treatment, pollution control, and other fields (Fellmann et al 2017;Khadempar et al 2019;Feng et al 2020). Thus, this system offers an excellent integration point for development of D. salina bioreactor.…”

Section: Introductionmentioning

confidence: 99%

CRISPR/Cas9-induced β-carotene hydroxylase mutation in Dunaliella salina CCAP19/18

Feng

Liang

et al. 2021

AMB Expr

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Dunaliella salina (D. salina) has been exploited as a novel expression system for the field of genetic engineering. However, owing to the low or inconsistent expression of target proteins, it has been greatly restricted to practical production of recombinant proteins. Since the accurate gene editing function of clustered regularly interspaced short palindromic repeat (CRISPR)/Cas system, β-carotene hydroxylase gene was chosen as an example to explore D. salina application with the purpose of improving expression level of foreign genes. In this paper, based on pKSE401 backbone, three CRISPR/Cas9 binary vectors were constructed to targeting exon 1 and 3 of the β-carotene hydroxylase of D. salina CCAP19/18 (Dschyb). D. salina mutants were obtained by salt gradient transformation method, and the expression of Dschyb gene were identified through real-time fluorescent quantitative PCR. Moreover, carotenoids content was analyzed by high-performance liquid chromatography at different time points after high intensity treatment. Compared with wild type strains, the β-carotene levels of mutants showed a significant increase, nearly up to 1.4 μg/ml, and the levels of zeaxanthin decreased to various degrees in mutants. All the results provide a compelling evidence for targeted gene editing in D. salina. This study gave a first successful gene editing of D. salina which has a very important practical significance for increasing carotene yield and meeting realistic industry demand. Furthermore, it provides an approach to overcome the current obstacles of D. salina, and then gives a strong tool to facilitates the development and application of D. salina system.

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“…D. salina cells have organelles such as nucleus, membrane, mitochondria, vacuoles, and golgi. In addition to chlorophyll A and B, carotene A and B, neoxanthin, kemanantin, lutein, and useful carotenoid pigments similar to zeaxanthin are involved among the intracellular compounds of D. salina species [23,24]. D. salina microalgae species are essential as phytoplankton, especially in saltwater, marshes and wetlands.…”

Section: Microalgae Production Systemsmentioning

confidence: 99%

Use of <em>Dunaliella salina</em> in Environmental Applications <sup>†</sup>

Çelebi

Bahadır

Şimşek

et al. 2021

Proceedings of 1st International Electronic Conference on Biological Diversity, Ecology and Evolution

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A macro and microalgae are widely used in environmental and biotechnological applications due to their unique natural properties. Algae groups are aquatic organisms that can be found in many parts of the world and vary in size from 3-10 μ to 70 cm. Algae are divided into two as prokaryotic (microalgae) and eukaryotic (macroalgae) according to their biological formations. "Cyanophyta" as microalgae and "Phaeophyta, Rhodophyta, Chlorophyta, Flagelleta" as macro algae are known. Dunaliella salina is a living thing that can live in saltwater ecosystems and belong to the microgreen algae group. Since these algae are a natural source of beta-carotene, they are of particular importance in the cosmetics and food industries compared to other green microalgae species. Almost all algae groups are used for different purposes in a wide variety of sectors. Dunaliella spp. types are used extensively in areas such as the energy sector (bio fuel), cosmetics, medical applications, bioplastic production, wastewater treatment, food industry. In addition, the most basic effect parameter of pink color formation in lakes with salt content due to seasonal changes is D. salina, which is not an environmental problem. Increasing environmental pollution, unconscious energy consumption, and climate change have led countries to seek alternative solutions to environmental issues and to develop environmentally friendly-technological methods. For sustainable environmental management and minimization of pollution; The benefits of using algae species have been demonstrated by different applications. In the literature, D. salina has focused on algae production and the use of pigments in the cosmetics and food industry. The aim of this study is to investigate the recent researches on D. salina and reveal the importance of this algae, especially in terms of sustainable environment and energy.

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CRISPR/Cas technology promotes the various application of Dunaliella salina system

Cited by 22 publications

References 114 publications

Multiomics approaches and genetic engineering of metabolism for improved biorefinery and wastewater treatment in microalgae

Multiomics approaches and genetic engineering of metabolism for improved biorefinery and wastewater treatment in microalgae

CRISPR/Cas9-induced β-carotene hydroxylase mutation in Dunaliella salina CCAP19/18

Use of <em>Dunaliella salina</em> in Environmental Applications <sup>†</sup>

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CRISPR/Cas technology promotes the various application of Dunaliella salina system

Cited by 22 publications

References 114 publications

Multiomics approaches and genetic engineering of metabolism for improved biorefinery and wastewater treatment in microalgae

Multiomics approaches and genetic engineering of metabolism for improved biorefinery and wastewater treatment in microalgae

CRISPR/Cas9-induced β-carotene hydroxylase mutation in Dunaliella salina CCAP19/18

Use of &lt;em&gt;Dunaliella salina&lt;/em&gt; in Environmental Applications &lt;sup&gt;†&lt;/sup&gt;

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Use of <em>Dunaliella salina</em> in Environmental Applications <sup>†</sup>