CRISPR-Cas9 based stress tolerance: New hope for abiotic stress tolerance in chickpea (Cicer arietinum)

Razzaq, Muhammad Khuram; Akhter, Muhammad Atique; Ahmad, Ramala Masood; Cheema, Kaiser Latif; Hina, Aiman; Karikari, Benjamin; Raza, Ghulam; Xing, Guangnan; Gai, Junyi; Khurshid, Mohsin

doi:10.1007/s11033-022-07391-4

Cited by 24 publications

(11 citation statements)

References 106 publications

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“…Although the CRISPR/Cas systems exhibit powerful ability in crop genetic improvement, some limitations still need to be overcome in this field [ 195 , 196 , 197 , 198 , 199 , 200 ]. SpCas9 requires a 5′-NGG-3ʹPAM immediately adjacent to the 20 nt DNA target sequence because it can only recognize NGG PAM sites, which limits its effectiveness.…”

Section: Crispr/cas Systems Of Advances Limitations and Prospective A...mentioning

confidence: 99%

CRISPR/Cas Genome Editing Technologies for Plant Improvement against Biotic and Abiotic Stresses: Advances, Limitations, and Future Perspectives

Wang

Zafar

Ali

et al. 2022

Cells

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Crossbreeding, mutation breeding, and traditional transgenic breeding take much time to improve desirable characters/traits. CRISPR/Cas-mediated genome editing (GE) is a game-changing tool that can create variation in desired traits, such as biotic and abiotic resistance, increase quality and yield in less time with easy applications, high efficiency, and low cost in producing the targeted edits for rapid improvement of crop plants. Plant pathogens and the severe environment cause considerable crop losses worldwide. GE approaches have emerged and opened new doors for breeding multiple-resistance crop varieties. Here, we have summarized recent advances in CRISPR/Cas-mediated GE for resistance against biotic and abiotic stresses in a crop molecular breeding program that includes the modification and improvement of genes response to biotic stresses induced by fungus, virus, and bacterial pathogens. We also discussed in depth the application of CRISPR/Cas for abiotic stresses (herbicide, drought, heat, and cold) in plants. In addition, we discussed the limitations and future challenges faced by breeders using GE tools for crop improvement and suggested directions for future improvements in GE for agricultural applications, providing novel ideas to create super cultivars with broad resistance to biotic and abiotic stress.

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Section: Crispr/cas Systems Of Advances Limitations and Prospective A...mentioning

confidence: 99%

CRISPR/Cas Genome Editing Technologies for Plant Improvement against Biotic and Abiotic Stresses: Advances, Limitations, and Future Perspectives

Wang

Zafar

Ali

et al. 2022

Cells

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“…Soybean tolerance to heat stress is often limited because of the complexity of its genome. With time, the emergence of novel gene-editing tools like CRISPR/Cas9 broke all biological barriers ( Ghosh and Dey, 2022 ; Rasheed et al, 2022a ; Razzaq et al, 2022 ). Increased heat waves threaten food security and damage the soybean crop on large areas ( Ahmad et al, 2021 ; Chennakesavulu et al, 2021 ; Camerlengo et al, 2022 ; Pandita, 2022 ).…”

Section: Crispr/cas9 Role In Heat Tolerancementioning

confidence: 99%

Improvement of heat stress tolerance in soybean (Glycine max L), by using conventional and molecular tools

et al. 2022

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The soybean is a significant legume crop, providing several vital dietary components. Extreme heat stress negatively affects soybean yield and quality, especially at the germination stage. Continuous change in climatic conditions is threatening the global food supply and food security. Therefore, it is a critical need of time to develop heat-tolerant soybean genotypes. Different molecular techniques have been developed to improve heat stress tolerance in soybean, but until now complete genetic mechanism of soybean is not fully understood. Various molecular methods, like quantitative trait loci (QTL) mapping, genetic engineering, transcription factors (TFs), transcriptome, and clustered regularly interspaced short palindromic repeats (CRISPR), are employed to incorporate heat tolerance in soybean under the extreme conditions of heat stress. These molecular techniques have significantly improved heat stress tolerance in soybean. Besides this, we can also use specific classical breeding approaches and different hormones to reduce the harmful consequences of heat waves on soybean. In future, integrated use of these molecular tools would bring significant results in developing heat tolerance in soybean. In the current review, we have presented a detailed overview of the improvement of heat tolerance in soybean and highlighted future prospective. Further studies are required to investigate different genetic factors governing the heat stress response in soybean. This information would be helpful for future studies focusing on improving heat tolerance in soybean.

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“…Biotic and abiotic pressures are major obstructions in chickpea production. Globally, abiotic stresses cause annual chickpea yield losses, either individually or in combination, which culminates in severe financial penalties [ 66 ]. Nearly 90% of the world’s chickpeas are produced in rainfed environments, where the plants are subjected to terminal drought stress and grown in soil whose water content is quickly diminishing.…”

Section: Abiotic and Biotic Constraints To Chickpea Productionmentioning

confidence: 99%

“…Biotic and abiotic pressures are major constraints on the productivity of chickpeas. Individual pests, diseases, and weeds are predicted to cause yield losses of 50–100 percent in tropical regions and 5–10 percent in temperate zones [ 66 , 75 ]. As a result, the market price of this pulse crop has skyrocketed, making it difficult for a big portion of India’s rural people to cope.…”

Section: Abiotic and Biotic Constraints To Chickpea Productionmentioning

confidence: 99%

“…The primary restrictions on chickpea output worldwide are abiotic stresses (terminal drought and heat stress), and they are anticipated to get worse because of climate unpredictability and change [ 66 ]. The efficiency of breeding programs has been improved via concerted efforts to create cultivars that are quickly resistant to abiotic stress.…”

Section: Abiotic and Biotic Constraints To Chickpea Productionmentioning

confidence: 99%

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Chickpea (Cicer arietinum L.) Biology and Biotechnology: From Domestication to Biofortification and Biopharming

Koul

Sharma

Sehgal

et al. 2022

Plants

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Chickpea (Cicer arietinum L.), the world’s second most consumed legume crop, is cultivated in more than 50 countries around the world. It is a boon for diabetics and is an excellent source of important nutrients such as vitamins A, C, E, K, B1–B3, B5, B6, B9 and minerals (Fe, Zn, Mg and Ca) which all have beneficial effects on human health. By 2050, the world population can cross 9 billion, and in order to feed the teaming millions, chickpea production should also be increased, as it is a healthy alternative to wheat flour and a boon for diabetics. Moreover, it is an important legume that is crucial for food, nutrition, and health security and the livelihood of the small-scale farmers with poor resources, in developing countries. Although marvelous improvement has been made in the development of biotic and abiotic stress-resistant varieties, still there are many lacunae, and to fulfill that, the incorporation of genomic technologies in chickpea breeding (genomics-assisted breeding, high-throughput and precise-phenotyping and implementation of novel breeding strategies) will facilitate the researchers in developing high yielding, climate resilient, water use efficient, salt-tolerant, insect/pathogen resistant varieties, acceptable to farmers, consumers, and industries. This review focuses on the origin and distribution, nutritional profile, genomic studies, and recent updates on crop improvement strategies for combating abiotic and biotic stresses in chickpea.

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CRISPR-Cas9 based stress tolerance: New hope for abiotic stress tolerance in chickpea (Cicer arietinum)

Cited by 24 publications

References 106 publications

CRISPR/Cas Genome Editing Technologies for Plant Improvement against Biotic and Abiotic Stresses: Advances, Limitations, and Future Perspectives

CRISPR/Cas Genome Editing Technologies for Plant Improvement against Biotic and Abiotic Stresses: Advances, Limitations, and Future Perspectives

Improvement of heat stress tolerance in soybean (Glycine max L), by using conventional and molecular tools

Chickpea (Cicer arietinum L.) Biology and Biotechnology: From Domestication to Biofortification and Biopharming

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