CRISPR/Cas9 Genome Editing in Bread Wheat (Triticum aestivum L.) Genetic Improvement

Dayani, Soleyman; Rahimmalek, Mehdi; Mazaheri-Tirani, Maryam

doi:10.1007/978-3-030-23108-8_12

Cited by 7 publications

(5 citation statements)

References 63 publications

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“…Using CRISPR-Cas9 RNPs to induce mutations in the polyphenol oxidase 2 gene (StPPO2) in tetraploid potato led to a significant decrease in enzymatic browning following cutting (González et al, 2020). Earlier, genome editing through CRISPR-Cas9 for the improvement of grain quality (important trait for consumers) including grain and kernel weight and storability in hexaploid wheat have been studied by Dayani et al (2019). PolysgRNAs tRNA-mediated genome editing may be used to specifically target the existence of multiple copy numbers in polyploidy crops.…”

Section: Ploidy Levelmentioning

confidence: 99%

CRISPR-Cas: A robust technology for enhancing consumer-preferred commercial traits in crops

et al. 2023

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The acceptance of new crop varieties by consumers is contingent on the presence of consumer-preferred traits, which include sensory attributes, nutritional value, industrial products and bioactive compounds production. Recent developments in genome editing technologies provide novel insight to identify gene functions and improve the various qualitative and quantitative traits of commercial importance in plants. Various conventional as well as advanced gene-mutagenesis techniques such as physical and chemical mutagenesis, CRISPR-Cas9, Cas12 and base editors are used for the trait improvement in crops. To meet consumer demand, breakthrough biotechnologies, especially CRISPR-Cas have received a fair share of scientific and industrial interest, particularly in plant genome editing. CRISPR-Cas is a versatile tool that can be used to knock out, replace and knock-in the desired gene fragments at targeted locations in the genome, resulting in heritable mutations of interest. This review highlights the existing literature and recent developments in CRISPR-Cas technologies (base editing, prime editing, multiplex gene editing, epigenome editing, gene delivery methods) for reliable and precise gene editing in plants. This review also discusses the potential of gene editing exhibited in crops for the improvement of consumer-demanded traits such as higher nutritional value, colour, texture, aroma/flavour, and production of industrial products such as biofuel, fibre, rubber and pharmaceuticals. In addition, the bottlenecks and challenges associated with gene editing system, such as off targeting, ploidy level and the ability to edit organelle genome have also been discussed.

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Section: Ploidy Levelmentioning

confidence: 99%

CRISPR-Cas: A robust technology for enhancing consumer-preferred commercial traits in crops

et al. 2023

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“…Several other genome editing studies for the improvement of various traits including grain and kernel weight and storability in wheat have been described and reviewed by Dayani et al (2019). Efficient multiplex genome editing targeting three different genes simultaneously in bread wheat was described recently (Li et al 2020b).…”

Section: Genome Editing and Polyploid Cropsmentioning

confidence: 99%

Genome editing of polyploid crops: prospects, achievements and bottlenecks

2021

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Plant breeding aims to develop improved crop varieties. Many crops have a polyploid and often highly heterozygous genome, which may make breeding of polyploid crops a real challenge. The efficiency of traditional breeding based on crossing and selection has been improved by using marker-assisted selection (MAS), and MAS is also being applied in polyploid crops, which helps e.g. for introgression breeding. However, methods such as random mutation breeding are difficult to apply in polyploid crops because there are multiple homoeologous copies (alleles) of each gene. Genome editing technology has revolutionized mutagenesis as it enables precisely selecting targets. The genome editing tool CRISPR/Cas is especially valuable for targeted mutagenesis in polyploids, as all alleles and/or copies of a gene can be targeted at once. Even multiple genes, each with multiple alleles, may be targeted simultaneously. In addition to targeted mutagenesis, targeted replacement of undesirable alleles by desired ones may become a promising application of genome editing for the improvement of polyploid crops, in the near future. Several examples of the application of genome editing for targeted mutagenesis are described here for a range of polyploid crops, and achievements and bottlenecks are highlighted.

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“…Since its first application as a plant genome editing technique [ 120 , 133 , 134 ], CRISPR-Cas has been widely applied in crop improvement programs [ 135 , 136 ]. Major crops that have benefited from the CRISPR-Cas technique include rice [ 32 , 33 , 34 , 35 ], maize [ 30 , 31 ], wheat [ 30 , 36 ] and other monocots [ 38 ]. In rice ( Oryza sativa ), the CRISPR-Cas system has been used to enhance drought [ 39 ], cold [ 40 ] and salt [ 41 , 42 ] tolerance, and to boost productivity [ 39 ].…”

Section: Potential Of Genome Editing Technologies For Tef Improvementmentioning

confidence: 99%

“…Among these strategies, genome editing techniques have recently received increased attention. Previous studies have suggested that the productivity of many cereal crops such as maize [ 30 , 31 ], rice [ 32 , 33 , 34 , 35 ], wheat [ 30 , 36 ] and other monocots [ 37 , 38 ] have been improved using the clustered regularly interspaced short palindromic repeats (CRISPR) system. In rice ( Oryza sativa L.), CRISPR-associated proteins (CRISPR-Cas) systems have been used to improve tolerance to drought [ 39 ], cold [ 40 ] and salt stress [ 41 , 42 ], ultimately boosting productivity [ 39 ].…”

Section: Introductionmentioning

confidence: 99%

From Traditional Breeding to Genome Editing for Boosting Productivity of the Ancient Grain Tef [Eragrostis tef (Zucc.) Trotter]

Numan

Khan

Asaf

et al. 2021

Plants

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Tef (Eragrostis tef (Zucc.) Trotter) is a staple food crop for 70% of the Ethiopian population and is currently cultivated in several countries for grain and forage production. It is one of the most nutritious grains, and is also more resilient to marginal soil and climate conditions than major cereals such as maize, wheat and rice. However, tef is an extremely low-yielding crop, mainly due to lodging, which is when stalks fall on the ground irreversibly, and prolonged drought during the growing season. Climate change is triggering several biotic and abiotic stresses which are expected to cause severe food shortages in the foreseeable future. This has necessitated an alternative and robust approach in order to improve resilience to diverse types of stresses and increase crop yields. Traditional breeding has been extensively implemented to develop crop varieties with traits of interest, although the technique has several limitations. Currently, genome editing technologies are receiving increased interest among plant biologists as a means of improving key agronomic traits. In this review, the potential application of clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (CRISPR-Cas) technology in improving stress resilience in tef is discussed. Several putative abiotic stress-resilient genes of the related monocot plant species have been discussed and proposed as target genes for editing in tef through the CRISPR-Cas system. This is expected to improve stress resilience and boost productivity, thereby ensuring food and nutrition security in the region where it is needed the most.

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CRISPR/Cas9 Genome Editing in Bread Wheat (Triticum aestivum L.) Genetic Improvement

Cited by 7 publications

References 63 publications

CRISPR-Cas: A robust technology for enhancing consumer-preferred commercial traits in crops

CRISPR-Cas: A robust technology for enhancing consumer-preferred commercial traits in crops

Genome editing of polyploid crops: prospects, achievements and bottlenecks

From Traditional Breeding to Genome Editing for Boosting Productivity of the Ancient Grain Tef [Eragrostis tef (Zucc.) Trotter]

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