CRISPR-Cas9 system: A genome-editing tool with endless possibilities

Tyagi, Swati; Kumar, Robin; Das, Ashim; Won, So Youn; Shukla, Pratyoosh

doi:10.1016/j.jbiotec.2020.05.008

Cited by 46 publications

(23 citation statements)

References 219 publications

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“…CRISPR/Cas system is continuously evolving by introducing the new Cas variants such as Cas9, Cas10, Cas12, and Cas13 orthologs into its toolbox and overcome the drawback of zinc-finger nucleases (ZFNs) and transcriptional activator-like effector nucleases (TALENs) (Puchta, 2017 ). Also, multiplex genome editing, base editing, DNA-free genome editing, and prime editing have opened new horizons in crop genome editing (Tyagi et al, 2020 ). These innovations in CRISPR/Cas system improve the editing efficiency by offering novel features like various protospacer adjacent motif (PAM) sites, small protein size, target specificity, low off-target effect, and efficient transformation techniques.…”

Section: De-novo Domestication Via Genome Editing Can Be a Promising Strategy For Salt Tolerancementioning

confidence: 99%

De-novo Domestication for Improving Salt Tolerance in Crops

et al. 2021

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Global agriculture production is under serious threat from rapidly increasing population and adverse climate changes. Food security is currently a huge challenge to feed 10 billion people by 2050. Crop domestication through conventional approaches is not good enough to meet the food demands and unable to fast-track the crop yields. Also, intensive breeding and rigorous selection of superior traits causes genetic erosion and eliminates stress-responsive genes, which makes crops more prone to abiotic stresses. Salt stress is one of the most prevailing abiotic stresses that poses severe damages to crop yield around the globe. Recent innovations in state-of-the-art genomics and transcriptomics technologies have paved the way to develop salinity tolerant crops. De novo domestication is one of the promising strategies to produce superior new crop genotypes through exploiting the genetic diversity of crop wild relatives (CWRs). Next-generation sequencing (NGS) technologies open new avenues to identifying the unique salt-tolerant genes from the CWRs. It has also led to the assembly of highly annotated crop pan-genomes to snapshot the full landscape of genetic diversity and recapture the huge gene repertoire of a species. The identification of novel genes alongside the emergence of cutting-edge genome editing tools for targeted manipulation renders de novo domestication a way forward for developing salt-tolerance crops. However, some risk associated with gene-edited crops causes hurdles for its adoption worldwide. Halophytes-led breeding for salinity tolerance provides an alternative strategy to identify extremely salt tolerant varieties that can be used to develop new crops to mitigate salinity stress.

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Section: De-novo Domestication Via Genome Editing Can Be a Promising Strategy For Salt Tolerancementioning

confidence: 99%

De-novo Domestication for Improving Salt Tolerance in Crops

et al. 2021

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“…Furthermore, the off-target activity is minimized compared with the other two methods [48]. The use of CRISPR-Cas9 is a simple but powerful genome editing tool, with various implementations, and their impact on new research trends has been reviewed elsewhere [49].…”

Section: The Crispr-cas System As a Genome Editing Toolmentioning

confidence: 99%

Advances in Editing Silkworms (Bombyx mori) Genome by Using the CRISPR-Cas System

Baci

Cucu

Giurgiu

et al. 2021

Insects

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CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated) represents a powerful genome editing technology that revolutionized in a short period of time numerous natural sciences branches. Therefore, extraordinary progress was made in various fields, such as entomology or biotechnology. Bombyx mori is one of the most important insects, not only for the sericulture industry, but for numerous scientific areas. The silkworms play a key role as a model organism, but also as a bioreactor for the recombinant protein production. Nowadays, the CRISPR-Cas genome editing system is frequently used in order to perform gene analyses, to increase the resistance against certain pathogens or as an imaging tool in B. mori. Here, we provide an overview of various studies that made use of CRISPR-Cas for B. mori genome editing, with a focus on emphasizing the high applicability of this system in entomology and biological sciences.

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“…However, recent editing tools are focused on a clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 and its derivatives. The sequence-specific target programming capacity of Cas9 has made it a powerful and indispensable genome-editing tool ( Tyagi et al, 2020 ). In B. subtilis , several highly efficient genome-editing protocols using the CRISPR-Cas9 ( Altenbuchner, 2016 ; Westbrook et al, 2016 ; So et al, 2017 ), and a subsequent serial genome-editing method for multiple targets have been reported ( Lim and Choi, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Cell Factory Engineering of Undomesticated Bacillus Strains Using a Modified Integrative and Conjugative Element for Efficient Plasmid Delivery

Jeong

Kim

et al. 2022

Front. Microbiol.

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A large number of Bacillus strains have been isolated from various environments and many of them have great potential as cell factories. However, they have been rarely developed as cell factories due to their poor transformation efficiency. In this study, we developed a highly efficient plasmid delivery system for undomesticated Bacillus strains using a modified integrative and conjugative element (MICE), which was designed to be activated by an inducer, prevent self-transfer, and deliver desired plasmids to the recipient cells. The MICE system was demonstrated to successfully introduce a gfp-containing plasmid into all 41 undomesticated Bacillus subtilis strains tested and eight other Bacillus species. The MICE was used to deliver a cytosine base editor (CBE)-based multiplex genome-editing tool for the cell factory engineering of the Bacillus species. The introduced CBE enabled one-step inactivation of the major extracellular protease genes of the tested strains. The engineered strains were used as hosts for heterologous expression of nattokinase, which resulted in various enzyme expression levels. The results suggested that the MICE and CBE systems can be powerful tools for genetic engineering of undomesticated Bacillus strains, and greatly contribute to the expansion of the Bacillus cell factory.

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CRISPR-Cas9 system: A genome-editing tool with endless possibilities

Cited by 46 publications

References 219 publications

De-novo Domestication for Improving Salt Tolerance in Crops

De-novo Domestication for Improving Salt Tolerance in Crops

Advances in Editing Silkworms (Bombyx mori) Genome by Using the CRISPR-Cas System

Cell Factory Engineering of Undomesticated Bacillus Strains Using a Modified Integrative and Conjugative Element for Efficient Plasmid Delivery

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