CRISPR-mediated technology for seed oil improvement in rapeseed: Challenges and future perspectives

Ali, Essa; Zhang, Kewei

doi:10.3389/fpls.2023.1086847

Cited by 5 publications

References 41 publications

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CRISPR/Cas-Based Precision Breeding of Oilseed Rape (Brassica napus L.) – Recent Improvements

Boniecka

2023

A Roadmap for Plant Genome Editing

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The seeds of oilseed rape (Brassica napus L.) are used in large-scale production of one of the most health-promoting plant oils in the food industry, as well as for animal feed and biofuel production. Thus, increasing the yield of this crop is of crucial economic and ecological importance. However, conventional breeding programs are slow, laborious and time-consuming. Hence, along with the discovery of the possibility to apply CRISPR/Cas technology to edit plant genomes and to accelerate the breeding process, much effort has been put into applying this technology to study specific genes and biosynthetic pathways, especially in species with many gene copies such as B. napus. Here, recent improvements in generating CRISPR/Cas-induced mutations in the B. napus genome, delivering CRISPR/Cas reagents into oilseed rape plant cells, fast-checking the efficiency of targeted mutagenesis of CRISPR/Cas reagents, and oilseed rape transformation and regeneration procedures are described. Finally, new applications of CRISPR/Cas tools in oilseed rape precision breeding are discussed, focusing mainly on applications verified in field.

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CRISPR/Cas-Based Precision Breeding of Oilseed Rape (Brassica napus L.) – Recent Improvements

Boniecka

2023

A Roadmap for Plant Genome Editing

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Does DNA extraction affect the specificity of a PCR method claiming the specific detectability of a genome-edited plant?

Edelmann,

Savini,

Moor

et al. 2024

GM Crops & Food

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Under current EU legislation, genetically modified organisms (GMOs) and derived food and feed products must be authorized as GM food, feed, or seed and appropriate detection methods must be made available for use in official controls. A Real-Time PCR method has recently been published by Chhalliyil et al. claiming to be specific for the detection and identification of genome-edited oilseed rape (OSR) lines commercialized in North America. In a previous study, we have independently assessed this method in three reference laboratories for sensitivity, specificity, and robustness. We found that the method does not meet all the minimum performance requirements (MPR) for GMO testing in the EU, which contradicts the claims of the method developer. Here we show, in addition to the previously published method assessment study that a modified DNA extraction is not the reason for the contradictory findings and does not affect the specificity of the method. We also discuss the procedures recently proposed by the method developers for interpreting PCR results with high Cq values.

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Chia (Salvia hispanica L.), a functional ‘superfood’: new insights into its botanical, genetic and nutraceutical characteristics

Zare,

Fournier-Level,

Ebert

et al. 2024

Annals of Botany

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Background Chia (Salvia hispanica L.) seeds have become increasingly popular among health-conscious consumers due to their high content of ω-3 fatty acids, which provide various health benefits. Comprehensive chemical analyses of chia seeds’ fatty acids and proteins have been conducted, revealing their functional properties. Recent studies have confirmed the high ω-3 content of chia seed oil and have hinted at additional functional characteristics. Scope This review article aims to provide an overview of the botanical, morphological, and biochemical features of chia plants, seeds, and seed mucilage. Additionally, we discuss the recent developments in genetic and molecular research on chia, including the latest transcriptomic and functional studies that examine the genes responsible for chia fatty acid biosynthesis. In recent years, research on chia seeds has shifted its focus from studying the physicochemical characteristics and chemical composition of seeds to understanding the metabolic pathways and molecular mechanisms that contribute to their nutritional benefits. This has led to a growing interest in various pharmaceutical, nutraceutical, and agricultural applications of chia. In this context, we discuss the latest research on chia, as well as the questions that remain unanswered, and identify areas that require further exploration. Conclusions Nutraceutical compounds associated with significant health benefits including ω-3 PUFAs, proteins, and phenolic compounds with antioxidant activity have been measured in high quantities in chia seeds. However, comprehensive investigations through both in vitro experiments and in vivo animal and controlled human trials are expected to provide greater clarity on the medicinal, antimicrobial, and antifungal effects of chia seeds. The recently published genome of chia and gene editing technologies, such as CRISPR, facilitate functional studies deciphering molecular mechanisms of biosynthesis and metabolic pathways in this crop. This necessitates development of stable transformation protocols and creation of a publicly available lipid database, mutant collection, and large-scale transcriptomic datasets for chia.

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CRISPR-mediated technology for seed oil improvement in rapeseed: Challenges and future perspectives

Cited by 5 publications

References 41 publications

CRISPR/Cas-Based Precision Breeding of Oilseed Rape (Brassica napus L.) – Recent Improvements

CRISPR/Cas-Based Precision Breeding of Oilseed Rape (Brassica napus L.) – Recent Improvements

Does DNA extraction affect the specificity of a PCR method claiming the specific detectability of a genome-edited plant?

Chia (Salvia hispanica L.), a functional ‘superfood’: new insights into its botanical, genetic and nutraceutical characteristics

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