CRISPRi as a Tool to Repress Multiple Copies of Extracellular Polymeric Substances (EPS)-Related Genes in the Cyanobacterium Synechocystis sp. PCC 6803

Santos, Marina; Pacheco, Catarina C.; Yao, Lun; Hudson, Elton P.; Tamagnini, Paula

doi:10.3390/life11111198

Cited by 10 publications

(5 citation statements)

References 29 publications

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“…CRISPRi has emerged as a strategy to selectively inhibit photosynthetic genes. CRISPRi enables a particularly intriguing strategy for manipulation of photosynthetic proteins due to its reversibility and its potential to multiplex targets through incorporation of multiple guide RNAs [ 126 , 127 ]. In one example of this type of strategy, DNase-dead Cpf1 was introduced into S. elongatus UTEX 2973 as part of a CRISPRi system to target Photosystem I for inhibition [ 128 ].…”

Section: Use Of Crispr and Crispr Interference For Study Of Photosynt...mentioning

confidence: 99%

Advances in the Understanding of the Lifecycle of Photosystem II

Johnson

Pakrasi

2022

Microorganisms

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Photosystem II is a light-driven water-plastoquinone oxidoreductase present in cyanobacteria, algae and plants. It produces molecular oxygen and protons to drive ATP synthesis, fueling life on Earth. As a multi-subunit membrane-protein-pigment complex, Photosystem II undergoes a dynamic cycle of synthesis, damage, and repair known as the Photosystem II lifecycle, to maintain a high level of photosynthetic activity at the cellular level. Cyanobacteria, oxygenic photosynthetic bacteria, are frequently used as model organisms to study oxygenic photosynthetic processes due to their ease of growth and genetic manipulation. The cyanobacterial PSII structure and function have been well-characterized, but its lifecycle is under active investigation. In this review, advances in studying the lifecycle of Photosystem II in cyanobacteria will be discussed, with a particular emphasis on new structural findings enabled by cryo-electron microscopy. These structural findings complement a rich and growing body of biochemical and molecular biology research into Photosystem II assembly and repair.

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Section: Use Of Crispr and Crispr Interference For Study Of Photosynt...mentioning

confidence: 99%

Advances in the Understanding of the Lifecycle of Photosystem II

Johnson

Pakrasi

2022

Microorganisms

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“…Genes associated with nucleoside diphosphate saccharide production from the serine pathway were highly expressed in "M. sinica" at high nitrite input, providing materials for polymerization to EPS. 52 EPS is then secreted by the mechanism associated with increased expression of wza 53 and kpsM 54 providing a barrier from nitrite toxicity. 55 Similar genes are expressed at a low level (about 100−1000-fold less) in "Ca.…”

Section: Discussionmentioning

confidence: 99%

“…sinica” at high nitrite input, providing materials for polymerization to EPS . EPS is then secreted by the mechanism associated with increased expression of wza and kpsM providing a barrier from nitrite toxicity . Similar genes are expressed at a low level (about 100–1000-fold less) in “ Ca.…”

Section: Discussionmentioning

confidence: 99%

Microbial Niche Differentiation during Nitrite-Dependent Anaerobic Methane Oxidation

Nie

Xie

Tan

et al. 2023

Environ. Sci. Technol.

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Nitrite-dependent anaerobic methane oxidation (n-DAMO) has been demonstrated to play important roles in the global methane and nitrogen cycle. However, despite diverse n-DAMO bacteria widely detected in environments, little is known about their physiology for microbial niche differentiation. Here, we show the microbial niche differentiation of n-DAMO bacteria through long-term reactor operations combining genome-centered omics and kinetic analysis. With the same inoculum dominated by both species "Candidatus Methylomirabilis oxyfera" and "Candidatus Methylomirabilis sinica", n-DAMO bacterial population was shifted to "Ca. M. oxyfera" in a reactor fed with low-strength nitrite, but shifted to "Ca. M. sinica" with high-strength nitrite. Metatranscriptomic analysis showed that "Ca. M. oxyfera" harbored more complete function in cell chemotaxis, flagellar assembly, and two-component system for better uptake of nitrite, while "Ca. M. sinica" had a more active ion transport and stress response system, and more redundant function in nitrite reduction to mitigate nitrite inhibition. Importantly, the half-saturation constant of nitrite (0.057 mM vs 0.334 mM NO 2 − ) and inhibition thresholds (0.932 mM vs 2.450 mM NO 2 − ) for "Ca. M. oxyfera" vs "Ca. M. sinica", respectively, were highly consistent with genomic results. Integrating these findings demonstrated biochemical characteristics, especially the kinetics of nitrite affinity and inhibition determine niche differentiation of n-DAMO bacteria.

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“…It has been found that the genetic manipulation of cyanobacteria has a significant effect on EPS production. Therefore, a number of investigations have been carried out to modify Cyanobacterial EPSs' characteristics and production [83][84][85]. However, our understanding of the genetic and enzymatic systems associated with EPS formation is still a very small portion of what has been derived from the complete mechanism.…”

Section: Cyanobacterial Epsmentioning

confidence: 99%

Role of Bacteria-Derived Exopolysaccharides in Inflammatory Bowel Disease with a Special Focus on Cyanobacterial Exopolysaccharides

Manoharan,

Natesan,

Billamboz

et al. 2024

Applied Microbiology

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Instances of inflammatory bowel disease (IBD), a chronic inflammatory condition of the gastrointestinal tract, are rapidly increasing in western and newly industrialized countries. Exopolysaccharides (EPSs) are one of the strategies to enhance the gut microbiota and modulate the immune-inflammatory response deregulation in IBD patients. EPSs are produced by commensal bacteria such as Lactobacillus and Bifidobacterium. Additionally, Cyanobacteria species are promising sources of novel EPS and have potential pharmaceutical and therapeutic applications. The presence of uronic acids and sulphate groups in Cyanobacterial EPSs is an important factor that gives EPSs an anionic charge that is not seen in other prokaryotic species. This feature may impact their physico-chemical characteristics and biological properties. Additionally, Cyanobacterial EPSs have a wide range of biotechnological applications that include use as thickeners, stabilizers, and gelling agents in the food and pharmaceutical sectors. The present review focuses on the role of EPSs in IBD, with a special focus on EPSs derived from Cyanobacteria. This review also covers the biological properties of Cyanobacterial EPS in immuno-inflammatory responses and against pathogens as well as its role in biotechnological applications. Overall, Cyanobacterial EPSs have therapeutic potential against IBD due to their anti-inflammatory and immunoregulatory properties that can reduce inflammation and regulate the immune response and restore the gut microbiota of patients.

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CRISPRi as a Tool to Repress Multiple Copies of Extracellular Polymeric Substances (EPS)-Related Genes in the Cyanobacterium Synechocystis sp. PCC 6803

Cited by 10 publications

References 29 publications

Advances in the Understanding of the Lifecycle of Photosystem II

Advances in the Understanding of the Lifecycle of Photosystem II

Microbial Niche Differentiation during Nitrite-Dependent Anaerobic Methane Oxidation

Role of Bacteria-Derived Exopolysaccharides in Inflammatory Bowel Disease with a Special Focus on Cyanobacterial Exopolysaccharides

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