Critical points for the design and application of RNA silencing constructs for plant virus resistance

Akbarimotlagh, Masoud; Azizi, Abdolbaset; Shams‐Bakhsh, Masoud; Jafari, Morad; Ghasemzadeh, Aysan; Palukaitis, Peter

doi:10.1016/bs.aivir.2023.02.001

Cited by 2 publications

(1 citation statement)

References 256 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These sRNAs are generated as duplexes with 2-nt 3′ overhangs from longer dsRNA precursors or hairpin-like secondary structures, respectively, through the action of Dicer-like (DCL) enzymes [ 85 ] ( Figure 2 ). RNA silencing has been used to engineer complete resistance against tobamoviruses [ 86 , 87 , 88 , 89 , 90 , 91 ]. In the case of tobamoviruses, the small replicase subunit (122–130 kDa) could function as the viral suppressors of RNA silencing (VSR).…”

Section: Rna-targeting Mechanismsmentioning

confidence: 99%

Plant Immunity against Tobamoviruses

Zheng,

Li,

Liu

2024

Viruses

View full text Add to dashboard Cite

Tobamoviruses are a group of plant viruses that pose a significant threat to agricultural crops worldwide. In this review, we focus on plant immunity against tobamoviruses, including pattern-triggered immunity (PTI), effector-triggered immunity (ETI), the RNA-targeting pathway, phytohormones, reactive oxygen species (ROS), and autophagy. Further, we highlight the genetic resources for resistance against tobamoviruses in plant breeding and discuss future directions on plant protection against tobamoviruses.

show abstract

Section: Rna-targeting Mechanismsmentioning

confidence: 99%

Plant Immunity against Tobamoviruses

Zheng,

Li,

Liu

2024

Viruses

View full text Add to dashboard Cite

show abstract

Investigating the interactions of endornaviruses with each other and with other viruses in common bean, Phaseolus vulgaris

Brine,

Viswanathan,

Murphy

et al. 2023

Virol J

View full text Add to dashboard Cite

Background Plant viruses of the genus Alphaendornavirus are transmitted solely via seed and pollen and generally cause no apparent disease. It has been conjectured that certain plant endornaviruses may confer advantages on their hosts through improved performance (e.g., seed yield) or resilience to abiotic or biotic insult. We recently characterised nine common bean (Phaseolus vulgaris L.) varieties that harboured either Phaseolus vulgaris endornavirus (PvEV1) alone, or PvEV1 in combination with PvEV2 or PvEV1 in combination with PvEV2 and PvEV3. Here, we investigated the interactions of these endornaviruses with each other, and with three infectious pathogenic viruses: cucumber mosaic virus (CMV), bean common mosaic virus (BCMV), and bean common mosaic necrosis virus (BCMNV). Results In lines harbouring PvEV1, PvEV1 and PvEV2, or PvEV1, PvEV2 plus PvEV3, the levels of PvEV1 and PvEV3 RNA were very similar between lines, although there were variations in PvEV2 RNA accumulation. In plants inoculated with infectious viruses, CMV, BCMV and BCMNV levels varied between lines, but this was most likely due to host genotype differences rather than to the presence or absence of endornaviruses. We tested the effects of endornaviruses on seed production and seedborne transmission of infectious pathogenic viruses but found no consistent relationship between the presence of endornaviruses and seed yield or protection from seedborne transmission of infectious pathogenic viruses. Conclusions It was concluded that endornaviruses do not interfere with each other’s accumulation. There appears to be no direct synergy or competition between infectious pathogenic viruses and endornaviruses, however, the effects of host genotype may obscure interactions between endornaviruses and infectious viruses. There is no consistent effect of endornaviruses on seed yield or susceptibility to seedborne transmission of other viruses.

show abstract

Critical points for the design and application of RNA silencing constructs for plant virus resistance

Cited by 2 publications

References 256 publications

Plant Immunity against Tobamoviruses

Plant Immunity against Tobamoviruses

Investigating the interactions of endornaviruses with each other and with other viruses in common bean, Phaseolus vulgaris

Contact Info

Product

Resources

About