Critical role for the long non-coding RNA AFAP1-AS1 in the proliferation and metastasis of hepatocellular carcinoma

Xu, Lei; Zhou, Chen; Li, Renfeng; Liang, Zhiwei; Zhai, Wenlong; Zhao, Longshuan; Zhang, Shuijun

doi:10.1007/s13277-016-4858-8

Cited by 52 publications

(40 citation statements)

References 21 publications

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“…30,35,[40][41][42][43][44][45][46][47][48][49][50][51][52][53][54][55][56][57][58] In this review, the related clinicopathological characteristics and molecular functions of this lncRNA in cancers are presented in Tables 1 and 2.…”

Section: Identification Of Afap1-as1mentioning

confidence: 99%

AFAP1‐AS1: A novel oncogenic long non‐coding RNA in human cancers

Zhang

Xiao

et al. 2017

Cell Proliferation

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Section: Identification Of Afap1-as1mentioning

confidence: 99%

AFAP1‐AS1: A novel oncogenic long non‐coding RNA in human cancers

Zhang

Xiao

et al. 2017

Cell Proliferation

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“…Recent studies have shown that AFAP1-AS1 promoted cells proliferation and invasion in nasopharyngeal carcinoma, hepatocellular carcinoma, esophageal squamous cell carcinoma, gallbladder cancer [17, 19, 22, 23]. To investigate roles played by AFAP1-AS1 in CCA, we first examined the expression of AFAP1-AS1 in all 4 CCA cell lines (QBC939, CCLP1, HuCC-T1 and RBE) and the nonmalignant cholangiocyte cell line BEC.…”

Section: Resultsmentioning

confidence: 99%

“…The detailed procedure was performed as our previous described [19]. In brief, total RNA was extracted from cells using Trizol Reagent (Invitrogen).…”

Section: Methodsmentioning

confidence: 99%

“…CCLP1 and RBE cells with high endogenous AFAP1-AS1 were selected for silencing. Lentivirus packaging, cell infection and selection of puromycin-resistant cells were performed as our previous described [19]. …”

Section: Methodsmentioning

confidence: 99%

“…The effects of AFAP1-AS1 on cell migration and invasion were assessed using Transwell assays as our previous described [19]. In brief, 5×10 4 cells were seeded in triplicates onto a 24-well plate with Boyden chambers (BD Biosciences, San Jose, CA, USA) coated with Matrigel or without Matrigel.…”

Section: Methodsmentioning

confidence: 99%

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Long Noncoding RNA AFAP1-AS1 Promoted Tumor Growth and Invasion in Cholangiocarcinoma

Xu¹,

Zhou²,

Li³

et al. 2017

Cell Physiol Biochem

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Background: Long non-coding RNAs (lncRNAs) have been shown to play important roles in a wide range of pathophysiological processes, including cancer progression. Our previous study has shown that AFAP1-AS1 was upregulated and acted as an oncogene in hepatocellular carcinoma. However, the expression and biological functions of lncRNA AFAP1-AS1 in intrahepatic cholangiocarcinoma (CCA) remains largely unknown. Methods: The expression level of AFAP1-AS1 was measured in 56 pairs of human cholangiocarcinoma tumor tissues and corresponding adjacent normal bile duct tissues. The correlation between AFAP1-AS1 and the clinicopathological features were evaluated by chi-square test. The effects of AFAP1-AS1 on CCA cells were determined by CCK-8 assay, clone formation assay, flow cytometry and transwell assay. Finally, to determine the effect of AFAP1-AS1 on tumor growth in vivo, AFAP1-AS1 knockdowned CCLP-1 cells were subcutaneously into nude mice to evaluate tumor growth. Results: In this study, we found that lncRNA AFAP1-AS1 was increased in CCA tissues and patients with high AFAP1-AS1 expression had a shorter overall survival. SiRNA-mediated AFAP1-AS1 knockdown significantly decreased cell proliferation of the CCA cells, with downregulation of C-myc and Cycling D1 in vitro. Furthermore, AFAP1-AS1 silencing inhibited cell migration partly due to decrease the expression of MMP-2 and MMP-9. In addition, CCLP-1 cells with AFAP1-AS1 knockdown were injected into nude mice to investigate the effect of AFAP1-AS1 on the tumorigenesis in vivo. Conclusions: Taken together, our findings suggested that AFAP1-AS1 might promote the CCA progression and provided a novel potential therapeutic target for CCA.

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AFAP1‐AS1 is upregulated and promotes esophageal squamous cell carcinoma cell proliferation and inhibits cell apoptosis

et al. 2016

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Recent findings indicate that long noncoding RNAs (lncRNAs) were dysregulated in many kinds of tumors including esophageal squamous cell carcinoma (ESCC). LncRNA AFAP1‐AS1 was found to be upregulated in hepatocellular carcinoma (HCC), lung cancer, colorectal cancer, esophageal adenocarcinoma (EAC), pancreatic ductal adenocarcinoma, and nasopharyngeal carcinoma, while its clinical value and potential function in ESCC are still unknown. Expression of AFAP1‐AS1 was measured in 65 ESCC tissues and corresponding noncancerous tissues by quantitative real‐time polymerase chain reaction, which revealed that AFAP1‐AS1 expression was markedly elevated in ESCC tissues and significantly associated with advanced TNM stage (P = 0.004) and larger tumor size (P = 0.040). Moreover, by knocking down AFAP1‐AS1 expression in ESCC cells, the proliferation and colony‐forming ability were inhibited and cell apoptosis was induced. Our data indicated the first time that AFAP1‐AS1, a novel oncogene, was remarkably upregulated and played a critical role in the progression of ESCC.

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Critical role for the long non-coding RNA AFAP1-AS1 in the proliferation and metastasis of hepatocellular carcinoma

Cited by 52 publications

References 21 publications

AFAP1‐AS1: A novel oncogenic long non‐coding RNA in human cancers

AFAP1‐AS1: A novel oncogenic long non‐coding RNA in human cancers

Long Noncoding RNA AFAP1-AS1 Promoted Tumor Growth and Invasion in Cholangiocarcinoma

AFAP1‐AS1 is upregulated and promotes esophageal squamous cell carcinoma cell proliferation and inhibits cell apoptosis

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