Critical-sized bone defects regeneration using a bone-inspired 3D bilayer collagen membrane in combination with leukocyte and platelet-rich fibrin membrane (L-PRF): An in vivo study

Fahimipour, Farahnaz; Bastami, Farshid; Khoshzaban, Ahad; Jahangir, Shahrbanoo; Eslaminejad, Mohamadreza Baghaban; Khayyatan, Fahimeh; Safiaghdam, Hannaneh; Sadooghi, Yeganeh; Safa, Majid; Kashi, Tahereh Sadat Jafarzadeh; Dashtimoghadam, Erfan; Tayebi, Lobat

doi:10.1016/j.tice.2019.101326

Cited by 11 publications

(4 citation statements)

References 60 publications

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“…Our study supports previous research; for instance, rabbit PRF was tested in rabbits with a 3D bilayer collagen membrane reinforced with β-TCP, resulting in approximately 30% new bone formation after four weeks [33]. Also, when using rat PRF alone or in combination with a mineralized biomaterial, the rat calvaria defect models allowed bone formation of around 10% and 30%, respectively, after four weeks [23].…”

Section: Discussionsupporting

confidence: 89%

“…Preclinical research revealed that collagen-based scaffolds were functionalized with platelet-rich plasma for enhanced skin wound healing [32]. Moreover, bilayer collagen membranes reinforced with beta-tricalcium-phosphate particles in combination with rabbit PRF were used to evaluate bone regeneration in rabbits in vivo [33]. Among all the strategies introduced, the most commonly and clinically implemented concept is using PRF for the preparation of sticky bone, where bone grafts are combined with pieces of solid PRF and mixed up with liquid PRF.…”

Section: Introductionmentioning

confidence: 99%

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Human versus Rat PRF on Collagen Membranes: A Pilot Study of Mineralization in Rat Calvaria Defect Model

Apaza Alccayhuaman,

Heimel,

Tangl

et al. 2024

Bioengineering

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Platelet-rich fibrin, the coagulated plasma fraction of blood, is commonly used to support natural healing in clinical applications. The rat calvaria defect is a standardized model to study bone regeneration. It remains, however, unclear if the rat calvaria defect is appropriate to investigate the impact of human PRF (Platelet-Rich Fibrin) on bone regeneration. To this end, we soaked Bio-Gide® collagen membranes in human or rat liquid concentrated PRF before placing them onto 5 mm calvarial defects in Sprague Dawley rats. Three weeks later, histology and micro-computed tomography (μCT) were performed. We observed that the collagen membranes soaked with rat PRF show the characteristic features of new bone and areas of mineralized collagen matrix, indicated by a median mineralized volume of 1.5 mm3 (range: 0.9; 5.3 mm3). Histology revealed new bone growing underneath the membrane and hybrid bone where collagen fibers are embedded in the new bone. Moreover, areas of passive mineralization were observed. The collagen membranes soaked with human PRF, however, were devoid of histological features of new bone formation in the center of the defect; only occasionally, new bone formed at the defect margins. Human PRF (h-PRF) caused a median bone volume of 0.9 mm3 (range: 0.3–3.3 mm3), which was significantly lower than what was observed with rat PRF (r-PRF), with a BV median of 1.2 mm3 (range: 0.3–5.9 mm3). Our findings indicate that the rat calvaria defect model is suitable for assessing the effects of rat PRF on bone formation, but caution is warranted when extrapolating conclusions regarding the efficacy of human PRF.

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Human versus Rat PRF on Collagen Membranes: A Pilot Study of Mineralization in Rat Calvaria Defect Model

Apaza Alccayhuaman,

Heimel,

Tangl

et al. 2024

Bioengineering

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“…However, the stability of both PRF alone and PRF composites at long term studies are still unknown (79). Besides, advanced platelet-rich fibrin (A-PRF) and Leukocyte-platelet-rich fibrin (L-PRF) also have been used widely for bone tissue engineering (81)(82)(83)(84)(85)(86)(87). Fig.…”

Section: Development Of Fibrin Composite In Mimicking Bone Tissuementioning

confidence: 99%

Angiogenic and Osteogenic Properties of Fibrin in Bone Tissue Engineering

Maaruf¹,

Jusoh²

2022

MJMHS

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The bone scaffold has become a promising alternative in bone tissue engineering due to the limitation associated with current bone treatments. However, the selection of scaffold material that could accurately mimic the extracellular matrix of native tissue remains challenging. Owing to its biological origin properties, natural materials including fibrin are widely used as scaffold materials as compared to synthetic materials. Fibrin has been recognized as one of the appealing natural biopolymers, which possesses unique characteristic due to its natural formed nano-scaffold, which provide a temporary matrix that facilitates cellular activities of cells. Fibrin has shown remarkable effects over other biomaterials in inducing angiogenesis and osteogenesis in bone regeneration owing to its mechanical and biological properties. In this article, we highlight the significance of fibrin materials in facilitating bone regeneration. We focus on the manipulation of fibrin composition and on the recent developments of fibrin composites in enhancing osteogenesis and angiogenesis for bone healing.

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“…El adicionar NPs a las membranas favorece la durabilidad del andamio mejorando las características del microambiente como lo fue referido por Lee y colaboradores, quienes en su estudio concluyeron que el uso de una pequeña cantidad de nanopartículas de oro (AuNPs) induce la regeneración efectiva del tejido al permitir la circulación sanguínea a través de la regulación de la porosidad y el bloqueo de la infiltración de tejido (Lee et al, 2018). El estudio realizado por Fahimipou y colaboradores demostraron que el uso de un andamio con nano β-TCP (nβ-TCP) muestra resultados prometedores en la adhesión y proliferación celular así como diferenciación osteogé-nica (Fahimipour et al, 2020) así como en estudios reportados por Masoudi et al (2017), donde se afirmó que el uso de la membrana que contenía el 10% de nβ-TCP presentó mejor bioactividad, biodegradabilidad, propiedades mecánicas y proliferación celular en comparación con la membrana nanofibrosa PCL/PGS sin nanopartículas; no obstante, también señalaron que el aumentar la concentración al 15% las propiedades mecánicas resultaban deficientes. Otro caso en el que se habló de la importancia de las concentraciones fue el mencionado por Xia et al, donde concluyeron que con 3% de nanopartículas de óxido de hierro (IONP) el andamio duplicó su fuerza de flexión y tuvo la mayor promoción de la diferenciación osteogénica de las células madre, favoreciendo la propagación celular y la formación de matriz ósea por las células (Xia et al, 2019).…”

Section: Semanaunclassified

Tendencias en regeneración periodontal con el uso de nanopartículas: revisión sistemática de la literatura

Vargas-Ruíz

Serrano-Díaz

Hernández-Gómez

et al. 2022

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Se realizó una revisión sistemática de la literatura en las bases de datos ScienceDirect, PubMed y SciELO. Como criterios de inclusión se tomaron en cuenta artículos originales, de texto completo que incluyeran el uso de nanopartículas (0-100 nm) con fines de regeneración periodontal publicados de 2016 a mayo del 2022. Los términos de búsqueda fueron “Periodontal regeneration AND nanoparticles AND dental materials”. Se obtuvieron y analizaron 13 artículos que cumplieron los criterios de inclusión y exclusión. Se encontró que la nanohidroxiapatita tiene alto efecto regenerador, seguido de nanopartículas de dióxido de titanio, oro, fosfato de calcio, quitosano y óxido de hierro, entre otras. Estas nanopartículas mostraron efecto de regeneración periodontal de una a cuatro semanas promoviendo principalmente las propiedades de osteoinducción, citocompatibilidad, adherencia celular, degradabilidad, analgesia y biomineralización. La nanotecnología en el campo odontológico está emergiendo como una plataforma atractiva para la terapia avanzada en conjunto con los biomateriales dentales. En esta revisión se encontró que las nHA y TiO2NPs mostraron la mejor respuesta celular en el menor tiempo para obtener resultados regenerativos en periodoncia en comparación con otros tipos de NPs utilizadas. Aún hay muy pocas NPs analizadas con fines de regeneración periodontal, por lo que su estudio es un área emergente; sin olvidarnos de las posibles limitantes, consecuencias y efectos adversos a corto y largo plazo, los cuales deben ser estudiados con mayor profundidad.

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Critical-sized bone defects regeneration using a bone-inspired 3D bilayer collagen membrane in combination with leukocyte and platelet-rich fibrin membrane (L-PRF): An in vivo study

Cited by 11 publications

References 60 publications

Human versus Rat PRF on Collagen Membranes: A Pilot Study of Mineralization in Rat Calvaria Defect Model

Human versus Rat PRF on Collagen Membranes: A Pilot Study of Mineralization in Rat Calvaria Defect Model

Angiogenic and Osteogenic Properties of Fibrin in Bone Tissue Engineering

Tendencias en regeneración periodontal con el uso de nanopartículas: revisión sistemática de la literatura

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