Crocetin protects against oxidative damage in rat primary hepatocytes

Tseng, Tsui‐Hwa; Chu, Chia-Yih; Huang, Jinming; Shiow, Song-Jui; Wang, Chau‐Jong

doi:10.1016/0304-3835(95)03964-x

Cited by 177 publications

(95 citation statements)

References 30 publications

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“…Ameliorating effects of saffron on cisplatin-induced toxicity and changes in enzyme activities have also been reported El Daly, 1998). It is known that crocins and crocetin decrease the aflatoxin B 1 and dimethylnitrosamineinduced hepatotoxicity and protects rat primary hepatocytes against oxidative damage (Lin and Wang, 1986;Wang et al, 1991aWang et al, , 1991bTseng et al, 1995).…”

Section: Effect Of Saffron and Crocin On Mms-induced Dna Damagementioning

confidence: 99%

Protective Effect of Crocus sativus Stigma Extract and Crocin (trans-crocin 4) on Methyl Methanesulfonate–Induced DNA Damage in Mice Organs

Hosseinzadeh

Abootorabi

Sadeghnia

2008

DNA and Cell Biology

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This study was designed to examine the effect of aqueous extract of Crocus sativus stigmas (CSE) and crocin (trans-crocin 4) on methyl methanesulfonate (MMS)-induced DNA damage in multiple mice organs using comet assay. Adult male NMRI mice in different groups were treated with either physiological saline (10 mL=Kg, intraperitoneal [ip]), CSE (80 mg=Kg, ip), crocin (400 mg=Kg, ip), MMS (120 mg=Kg, ip), and CSE (5, 20, and 80 mg=Kg, ip) 45 min prior to MMS administration or crocin (50, 200, and 400 mg=Kg, ip) 45 min prior to MMS administration. Mice were scarified about 3 h after each different treatment, and the alkaline comet assay was used to evaluate the effect of these compounds on DNA damage in different mice organs. The percent of DNA in the comet tail (% tail DNA) was measured. A significant increase in the % tail DNA was seen in nuclei of different organs of MMS-treated mice. In control groups, no significant difference was found in the % tail DNA between CSE-or crocin-pretreated and saline-pretreated mice. The MMS-induced DNA damage in CSEpretreated mice (80 mg=Kg) was decreased between 2.67-fold (kidney) and 4.48-fold (lung) compared to those of MMS-treated animals alone ( p < 0.001). This suppression of DNA damage by CSE was found to be depended on the dose, which pretreatment with CSE (5 mg=Kg) only reduced DNA damage by 6.97%, 6.57%, 7.27%, and 9.90% in liver, lung, kidney, and spleen, respectively ( p > 0.05 as compared with MMS-treated group). In the same way, crocin also significantly decreased DNA damage by MMS (between 4.69-fold for liver and 6.55-fold for spleen, 400 mg=Kg), in a dose-dependent manner. These data indicate that there is a genoprotective property in CSE and crocin, as revealed by the comet assay, in vivo.

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Section: Effect Of Saffron and Crocin On Mms-induced Dna Damagementioning

confidence: 99%

Protective Effect of Crocus sativus Stigma Extract and Crocin (trans-crocin 4) on Methyl Methanesulfonate–Induced DNA Damage in Mice Organs

Hosseinzadeh

Abootorabi

Sadeghnia

2008

DNA and Cell Biology

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“…In addition, crocin and safranil have shown to increase the cleavage of caspase-3, arrest the cell cycle, and cause DNA damage in HepG2 cells. [85] Moreover, Tseng et al [86] reported that crocetin, a major constituent in saffron, was an anti-oxidant and hepatoprotective by decreasing the synthesis of malondialdehyde in hepatocytes, a marker for fatty acid oxidation and oxidative stress. As concluded by other researchers, crocetin protects against cancer by inducing apoptosis and arresting the cell cycle.…”

Section: Livistona Chinensis Seedmentioning

confidence: 99%

Natural products and hepatocellular carcinoma: a review

Alnajjar¹,

Elsiesy²

2015

Hepatoma Res

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Hepatocellular carcinoma (HCC) is the fi fth commonest cause of malignancy and the third cause of cancer mortality. There are different treatment options for HCC ranging from loco-regional therapy to surgical treatment. Different regimen of systemic chemotherapy has been tried with a poor response. Several studies aimed at discovering more molecules for the management of HCC. Those studies aimed at recognizing and targeting several signaling and molecular pathways that lead to cellular proliferation and tumor formation. In this review, we discussed the role of several agents found in natural and dietary products such as curcumin, resveratrol, fl avonoids, Rubus aleaefolius Poir total alkaloids, Livistona chinensis seed, and crocetin. We had used the names of the above-mentioned products as key words in addition to "HCC" on PubMed to fi nd studies that discussed their roles in HCC. Articles were downloaded for reviewing and discussing natural products that had adequate studies in treating HCC.

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“…Gardeniae Fructus (GF) is a traditional Chinese medicine, which has alleviating effect on cytotoxicity [20], antioxidant activity [21] and protective action on pancreatitis [22]. We have also reported [23,24] that GF composed in Bofutsushosan alleviates the abnormal glucose/lipid metabolism in STZ-diabetic mice.…”

Section: Q Yu Et Al 158mentioning

confidence: 99%

Anti-Hyperglycemic Effect of Single Administered Gardeniae Fructus in Streptozotocin-Induced Diabetic Mice by Improving Insulin Resistance and Enhancing Glucose Uptake in Skeletal Muscle

Yu¹,

Takahashi²,

Nomura³

et al. 2013

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The mechanisms of Gardeniae Fructus (GF) for anti-hyperglycemic action were demonstrated in streptozotocin (STZ)-diabetic mice. Six hours after single intraperitoneal administration of GF (300 mg/kg) or H 2 O into 3 hour-fasted STZ-diabetic mice, glucose and insulin tolerances were assessed by intraperitoneal glucose (1.5 g/kg) tolerance test (IPGTT) and intraperitoneal insulin (0.65 U/kg) tolerance test (IPITT), respectively. Effects of GF on insulin signaling pathways in soleus muscle such as glucose uptake, expression of glucose transporter 4 (GLUT4) in the plasma membrane and phosphorylation of Akt (P-Akt) in cytosolic fraction were examined in STZ-diabetic mice. In IPGTT test, GF significantly accelerated clearance of exogenous glucose and its glucose-lowering action was greater than H 2 O-treated control in STZ-diabetic mice. GF also promoted an exogenous glucose-increased insulin level in STZ-diabetic mice. In IPITT test, GF decreased glucose level to the greater extent than H 2 O-treated control in STZ-diabetic mice. Furthermore, GF significantly decreased high HOMA-IR in STZ-diabetic mice from 21.6 ± 2.4 to 12.4 ± 1.9 (mg/dl × μU/ml). These results implied that GF improved insulin resistance in STZ-diabetic mice. GF increased glucose uptake of soleus muscle 1.5 times greater than H 2 O-treated control in STZ-diabetic mice. GF enlarged insulin (10 nmol/ml)-increased glucose uptake to 1.8 time-greater. Correspondingly, GF increased expression of GLUT4 in the plasma membrane of soleus muscle to 1.4 time-greater, and P-Akt in the cytosolic fraction of soleus muscle to 1.9 time-greater than those in H 2 Otreated control. In conclusion, the improvement of GF on insulin resistance is associated with the repair of insulin signaling via P-Akt, GLUT4 and glucose uptake pathway in soleus muscle of STZ-diabetic mice.

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Crocetin protects against oxidative damage in rat primary hepatocytes

Cited by 177 publications

References 30 publications

Protective Effect of Crocus sativus Stigma Extract and Crocin (trans-crocin 4) on Methyl Methanesulfonate–Induced DNA Damage in Mice Organs

Protective Effect of Crocus sativus Stigma Extract and Crocin (trans-crocin 4) on Methyl Methanesulfonate–Induced DNA Damage in Mice Organs

Natural products and hepatocellular carcinoma: a review

Anti-Hyperglycemic Effect of Single Administered Gardeniae Fructus in Streptozotocin-Induced Diabetic Mice by Improving Insulin Resistance and Enhancing Glucose Uptake in Skeletal Muscle

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