Crocus sativus L. Extract Containing Polyphenols Modulates Oxidative Stress and Inflammatory Response against Anti-Tuberculosis Drugs-Induced Liver Injury

Abstract

The purpose of this study is to analyze the polyphenolic rich extract of Crocus sativus L. petals (CSP) in modulating liver oxidative stress and inflammatory response status against rifampicin isoniazid (INH-RIF) drug-induced liver injury. The INH-RIF was administered for 14 days with varying doses in Wistar rats, while silymarin was administered as standard dose. We report the defensive impacts of CSP against INH-RIF induced liver oxidative stress and proinflammatory cytokine. The CSP treatment at both doses significantly controlled all modulating biochemical hepatic injury indicators and resulted in the attenuation of arbitral INH-RIF damage. The components present in CSP identified by LC–ESI-Q-TOF–MS were found to be flavonoids and fatty acids. It can be inferred that CSP possesses a hepatoprotective capacity against INH-RIF-mediated hepatic injury, which may prove to be a medically beneficial natural product for the management of drug-induced liver injury.

Antioxidant, Antimicrobial, Antidiabetic and Cytotoxic Activity of Crocus sativus L. Petals

“…This variance in antimicrobial activity of extracts of saffron petals between the studies may possibly be due to the variation in phenolic composition and concentrations in the extracts used [26,47]. The results of the study provide evidence of C. sativus petals may be a possible source of potential antimicrobial agents [48]. Our results are similar to the earlier study, in which authors noticed a rise in antimicrobial activity of C. sativus extracts with increase in concentrations [49].…”

Protective efficiency of parsley (Petroselinum crispum) against oxidative stress, DNA damage and nephrotoxicity induced with anti- tuberculosis drugs

“…It was suggested to inhibit lipid peroxidation and scavenge hydroxide and superoxide radicals, thus improving the antioxidant battery, the active constituents present in PAE such as flavonoids and vitamin C. In this context, the protective role of PAE may be due to the antiperoxidant and antioxidant effects of its components. These findings are in line with the latest reports (Al-Seeni et al, 2018& Elkomy et al, 2020 In addition, the current study pointed out that the administration of ATB resulted in a substantial increase in the serum level of inflammatory cytokines (TNF alpha & IL-1β) as well as the percentage of DNA fragmentation; this finding is agonised by recent data from Wali et al (2020) and Shabbir et al (2020). TNF alpha and IL-1β are active pro-inflammatory cytokines and are significant mediators of damage to inflammatory tissue.…”

Crocus sativus L. Petal Extract Inhibits Inflammation and Osteoclastogenesis in RAW 264.7 Cell Model

“…In a 6-week double-blind, placebo-controlled and randomized trial of mild-to-moderate depression, patients that received capsules of Crocus sativus L. petals benefited from a significantly better outcome than those who received the placebo [13]; a hydroalcoholic extract of saffron petals displayed a protective effect against dysregulated ovarian steroids and inflammatory markers in a mouse model of testosterone-induced polycystic ovary syndrome [38]. Moreover, for saffron petal extracts, several studies documented the antioxidant and hepatoprotective activity in cell injury in vitro models [14,[39][40][41], and the anti-inflammatory effect both in vitro [31] and in vivo [16]. Notably, although many of these studies use experimental models involving pro-inflammatory immune responses, only two works have thus far investigated macrophages as one of the possible cell targets of Crocus sativus L. petal derivatives, showing that two purified polysaccharides, not a whole petal extract, augmented the immunogenic functions of macrophage RAW 264.7 cells [42,43].…”