Crohn’s disease recurrence updates: first surgery vs. surgical relapse patients display different profiles of ileal microbiota and systemic microbial-associated inflammatory factors

Russo, Edda; Cinci, Lorenzo; Gloria, Leandro Di; Baldi, Simone; D’Ambrosio, Mario; Nannini, Giulia; Bigagli, Elisabetta; Curini, Lavinia; Pallecchi, Marco; Arcese, Donato Andrea; Scaringi, Stefano; Malentacchi, Cecilia; Bartolucci, Gianluca; Ramazzotti, Matteo; Luceri, Cristina; Amedei, Amedeo; Giudici, Francesco

doi:10.3389/fimmu.2022.886468

Cited by 18 publications

(17 citation statements)

References 93 publications

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“…21 Our group have recently observed changes in the profiles of circulating FFA in various immune-mediated disorders, such as celiac disease, 19 systemic sclerosis 22 and Crohn's disease. 23 Likewise, our data have shown that the serum FFA signature in PV patients is significantly altered in comparison with the 'healthy profile' and displayed a shift towards a pro-inflammatory state. This 'pemphigus signature' could be summarized as follows: (i) a down-regulation of SCFA with anti-inflammatory functions, especially propionic and butyric acids and (ii) an up-regulation of pro-inflammatory MCFA and LCFA, including hexanoic and hexadecanoic acids.…”

Section: Discussionsupporting

confidence: 52%

Alterations of circulating free fatty acids in patients with pemphigus vulgaris

Maglie,

Baldi,

Nannini

et al. 2024

Experimental Dermatology

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Free fatty acids (FFA) have gained research interest owing to their functions in both local and systemic immune regulation. Changes in the serum levels of anti‐inflammatory short chain fatty acids (SCFA), primarily derived from the gut microbiota, and pro‐inflammatory medium (MCFA) and long (LCFA) chain fatty acids, derived from either the gut microbiota or the diet, have been associated with autoimmunity. Circulating FFA were retrospectively analysed by a gas chromatography–mass spectrometry method in the serum of 18 patients with pemphigus vulgaris (PV) at the baseline and 6 months (n = 10) after immunosuppressive treatments, and 18 healthy controls (HC). Circulating FFA were correlated with the Pemphigus Disease Area Index (PDAI) and serum concentrations of interferon‐gamma (IFN‐γ), Interleukin (IL)‐17A, IL‐5, IL‐10 and IL‐21. Principal Component analysis computed on FFA abundances revealed significant differences in the profile of SCFA (p = 0,012), MCFA (p = 0.00015) and LCFA (p = 0,035) between PV patients and HC, which were not significantly changed by immunosuppressive treatments. PV patients showed a significantly lower serum concentration of propionic (p < 0.0005) and butyric (p < 0.0005) acids, SCFA with anti‐inflammatory functions, while hexanoic (p < 0.0005) and hexadecanoic (p = 0.0006) acids, pro‐inflammatory MCFA and LCFA respectively, were over‐represented. Treatments induced a significant decrease of hexanoic (p = 0.035) and a further increase of hexadecanoic (p = 0.046) acids. Positive correlations emerged between IFN‐γ and acetic acid (Rho = 0.60), IFN‐γ and hexanoic acid (Rho = 0.46), IL‐5 and both hexadecanoic acid (Rho = 0.50) and octadecanoic acid (Rho = 0.53), butyric acid and PDAI (Rho = 0.53). PV was associated with a remarked imbalance of circulating FFA compared to HC. The serum alterations of SCFA, MCFA, and LCFA may contribute to promoting inflammation in PV. Deeper insights into the immunomodulatory functions of these molecules may pave the way for personalized dietary interventions in PV patients.

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Section: Discussionsupporting

confidence: 52%

Alterations of circulating free fatty acids in patients with pemphigus vulgaris

Maglie,

Baldi,

Nannini

et al. 2024

Experimental Dermatology

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“…НВК та ХК -не смертельні захворювання, але дуже виснажливі, з широким спектром симптомів, які містять лихоманку, ректальні кровотечі, блювання, діарею, біль у животі, анемію та втрату маси тіла. Хронічний характер симптомів призводить до зниження якості життя таких пацієнтів [1][2][3].…”

Section: вступunclassified

“…Жирні кислоти (ЖК) -це карбонові кислоти з аліфатичним ланцюгом, які залежно від довжини ланцюга класифікуються як коротко-, середньо-та довголанцюгові ЖК (КЛЖК, СЛЖК та ДЛЖК відповідно), а залежності від кількості подвійних зв'язків у ньому як насичені ЖК (НЖК) -без подвійних зв'язків, мононенасичені ЖК (МНЖК) -з одним подвійним зв'язком та поліненасичені ЖК (ПНЖК) -з двома або більше подвійними зв'язками [1,2,5]. До КЛЖК (C:2-C:5), які мають не більше ніж 5 атомів вуглецю у своєму складі, належать летка оцтова (C2:0), пропіонова (C3:0) та масляна (C4:0) ЖК [5].…”

Section: вступunclassified

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Prognostic value of blood saturated fatty acids in inflammatory bowel diseases

Stoykevich

Karachynova

Кленіна

et al. 2023

GASTRO

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Background. Pathogenetic factors that cause the development of inflammatory bowel diseases (IBD) remain poorly understood, namely, the peculiarities of saturated fatty acids (SFAs) in the blood serum at different degrees of disease severity, which is quite an important task. Objective: to evaluate serum level of SFAs depending on IBD severity. Materials and methods. Thirty-seven patients with IBD were examined, their average age was (38.5 ± 2.1) years. Depending on the severity of the disease, the patients were divided into 2 groups: group I — with IBD of moderate severity (n = 24) and group II — with severe IBD (n = 13). The control group consisted of 16 healthy people. The quantitative content of SFAs in the blood serum of the examined patients was determined by gas chromatography. Median (Me), lower (25%) and upper (75%) quartiles were used to describe the data. Results. Biochemical analysis revealed a tendency to decrease in the level of short-chain SFA (butyric acid; p > 0.05) and a significant increase in the total content of medium- and long-chain SFAs (MCSFAs and LCSFAs; p < 0.001) in the serum of group I and II patients compared to the controls. It was found that with increasing severity of IBD, there was a decrease in LCSFAs content in the blood (r = –0.420, p = 0.048). The serum spectrum of SFAs was analyzed and a significant increase in all MCSFAs fractions was detected in both groups of patients: caproic acid (p < 0.001), caprylic acid (p < 0.001), capric acid (p ≤ 0.002), undecylic acid (p ≤ 0.006) and lauric acid (p ≤ 0.001). Characteristically, the content of the most MCSFAs fractions had a tendency to decrease (p > 0.05) in group I against group II of patients. The content of LCSFAs, namely: tridecylic acid (p ≤ 0.012), myristic acid (p < 0.001), pentadecylic acid (p ≤ 0.012), palmitic acid (p < 0.001), stearic acid (p ≤ 0.001) and heneicosylic acid (p < 0.001), increased significantly in group I and II of patients, while the content of margaric and eicosanoic acids — only in group I compared to the controls. Almost all LCSFAs (except tridecylic acid) had a tendency to increase in group I against group II. Conclusions. It has been shown that the content of SFAs in the blood depends on the degree of IBD severity. The mechanism of SFAs action with different carbon chain lengths is multidirectional and is associated with the effect on pro-/anti-inflammatory mediators and with the maintenance of the immune and intestinal homeostasis. The necessity of determining serum SFAs in IBD to correct the identified disorders has been confirmed.

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“…Primer trimming, denoising, high-quality sequence merging and ASV delineation were performed using Cutadapt and DADA2 in the QIIME2 v2021.4 environment. The scikit-learn classifier retrained on the V3-V4 region of the SILVA138 database has been used for the taxonomic assignment [24]. Further details regarding sequence processing and analysis is available on GitHub (see https://github.com/LeandroD94/Papers).…”

Section: Feces Collection and 16s Rrna V3-v4 Region Gene Sequencing A...mentioning

confidence: 99%