Crop Improvement Through Microbial Biotechnology: A Cross Talk

Goyal, Khushboo; Kumar, Tarun; Sharma, Pinki; Rao, M. J. V.; Ahmed, Vasim; Chauhan, Nar Singh

doi:10.1007/978-981-13-8805-7_4

Cited by 10 publications

(8 citation statements)

References 119 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the current study, the isolated bacteria mainly belonged to the Gammaproteobacteria and Alphaproteobacteria , with Pantoea , Sphingomonas , Bacillus , Curtobacterium , Rhizobium , and Paenibacillus being some of the dominant bacterial genera isolated from G. max seed throughout the study. Notably, most of these genera have been characterized with a range of beneficial features such as the ability to fix nitrogen, indole-3-acetic acid production, and antagonistic abilities against various bacteria and fungi ( Silini-Cherif et al, 2012 ; Hansen et al, 2017 ; Goyal et al, 2019 ; Liu et al, 2019 ). Data indicated that the genera present with more than 0.1% relative abundance in undried seed remained culturable after 14months of storage under all conditions.…”

Section: Discussionmentioning

confidence: 99%

Implications of Seed Vault Storage Strategies for Conservation of Seed Bacterial Microbiomes

et al. 2021

View full text Add to dashboard Cite

Global seed vaults are important, as they conserve plant genetic resources for future breeding to improve crop yield and quality and to overcome biotic and abiotic stresses. However, little is known about the impact of standard storage procedures, such as seed drying and cold storage on the seed bacterial community, and the ability to recover seed-associated bacteria after storage. In this study, soybean [Glycine max (L.) Merr.] seeds were analyzed to characterize changes in the bacterial community composition and culturability under varying storage conditions. The G. max bacterial microbiome was analyzed from undried seed, dried seed, and seed stored for 0, 3, 6, and 14months. Storage temperatures consisted of −20°C, 4°C, and room temperature (RT), with −20°C being commonly used in seed storage vaults globally. The seed microbiome of G. max was dominated by Gammaproteobacteria under all conditions. Undried seed was dominated by Pantoea (33.9%) and Pseudomonas (51.1%); however, following drying, the abundance of Pseudomonas declined significantly (0.9%), Pantoea increased significantly (73.6%), and four genera previously identified including Pajaroellobacter, Nesterenkonia, env.OPS_17, and Acidibacter were undetectable. Subsequent storage at RT, 4, or −20°C maintained high-abundance Genera at the majority of time points, although RT caused greater fluctuations in abundances. For many of the low-abundance Genera, storage at −20°C resulted in their gradual disappearance, whereas storage at 4°C or RT resulted in their more rapid disappearance. The changes in seed bacterial composition were reflected by cultured bacterial taxa obtained from the stored G. max seed. The main taxa were largely culturable and had similar relative abundance, while many, but not all, of the low-abundance taxa were also culturable. Overall, these results indicate that the initial seed drying affects the seed bacterial composition, suggesting that microbial isolation prior to seed drying is recommended to conserve these microbes. The standard seed storage condition of −20°C is most suitable for conservation of the bacterial seed microbiome, as this storage temperature slows down the loss of seed bacterial diversity over longer time periods, particularly low-abundance taxa.

show abstract

Section: Discussionmentioning

confidence: 99%

Implications of Seed Vault Storage Strategies for Conservation of Seed Bacterial Microbiomes

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The main adaptation mechanisms for tolerating salinity stress include ion homeostasis, accumulation of osmolytes, and production of universal proteins related to salt stress tolerance ( Goyal et al, 2019 ). Na + /H + antiporters are a group of transmembrane proteins that exist in the plasma membrane of nearly all cells and participate significantly in the conservation of intracellular pH, cellular sodium amount, homeostasis, and volume of the cell ( Goyal et al, 2019 ). Five types of Na + /H + antiporters are present in prokaryotes, including NhaA, NhaB, NhaC, NhaD, and NapA.…”

Section: Molecular Basis Of the Stress Sensing And Halo Tolerance Mec...mentioning

confidence: 99%

“…Nhas perceive the ion concentration of the surroundings and moderate their action to preserve the homeostasis of cells ( Kapoor and Kanwar, 2019 ). NhaA antiporter acts very selectively to exclude Na + ( Goyal et al, 2019 ). NhaA can be applied as an applicable marker to screen the salt tolerant strains ( Kapoor and Kanwar, 2019 ).…”

Section: Molecular Basis Of the Stress Sensing And Halo Tolerance Mec...mentioning

confidence: 99%

“…Incitation of K + uptake is the first quick reaction to an osmotic change by bacteria ( Goyal et al, 2019 ). Three K + uptake systems have different affinities: the Kup system, which is permanently active and keeps a little level of K + absorption and is not regulated through osmolality, while Trk and Kdp systems are multipartite inducible systems, which activated afterward osmotic up shock ( Goyal et al, 2019 ). Kdp was the most common gene related to K + uptake systems, which has been identified in the genomes of 55% of assessed halotolerant PGPB ( Table 1 ).…”

Section: Molecular Basis Of the Stress Sensing And Halo Tolerance Mec...mentioning

confidence: 99%

See 1 more Smart Citation

Salinity stress endurance of the plants with the aid of bacterial genes

et al. 2023

View full text Add to dashboard Cite

The application of plant growth-promoting bacteria (PGPB) is vital for sustainable agriculture with continuous world population growth and an increase in soil salinity. Salinity is one of the severe abiotic stresses which lessens the productivity of agricultural lands. Plant growth-promoting bacteria are key players in solving this problem and can mitigate salinity stress. The highest of reported halotolerant Plant growth-promoting bacteria belonged to Firmicutes (approximately 50%), Proteobacteria (40%), and Actinobacteria (10%), respectively. The most dominant genera of halotolerant plant growth-promoting bacteria are Bacillus and Pseudomonas. Currently, the identification of new plant growth-promoting bacteria with special beneficial properties is increasingly needed. Moreover, for the effective use of plant growth-promoting bacteria in agriculture, the unknown molecular aspects of their function and interaction with plants must be defined. Omics and meta-omics studies can unreveal these unknown genes and pathways. However, more accurate omics studies need a detailed understanding of so far known molecular mechanisms of plant stress protection by plant growth-promoting bacteria. In this review, the molecular basis of salinity stress mitigation by plant growth-promoting bacteria is presented, the identified genes in the genomes of 20 halotolerant plant growth-promoting bacteria are assessed, and the prevalence of their involved genes is highlighted. The genes related to the synthesis of indole acetic acid (IAA) (70%), siderophores (60%), osmoprotectants (80%), chaperons (40%), 1-aminocyclopropane-1-carboxylate (ACC) deaminase (50%), and antioxidants (50%), phosphate solubilization (60%), and ion homeostasis (80%) were the most common detected genes in the genomes of evaluated halotolerant plant growth-promoting and salinity stress-alleviating bacteria. The most prevalent genes can be applied as candidates for designing molecular markers for screening of new halotolerant plant growth-promoting bacteria.

show abstract

“…Lucerne is important for agriculture and environment because of its ability to fix atmospheric N2, stop soil erosion, deliver food for animals, maintain soil organic matter, thus have a great contribution for sustainable agriculture and supplying food for animals (Kertikova, 2008;Yadav et al, 2018;Kosev & Kertikova, 2019). The soil used in this experiment have the active natural population for symbiotic N2 fixation bacteria thus can minimize mineral nitrogen application by using organic fertilizers and support yield formation of the following crop (Vasileva & Kostov, 2015).…”

Section: Introductionmentioning

confidence: 99%

Organic Fertilization of Lucerne and the Following Oat Crop in Non-Irrigated Conditions

Vasileva

Kostov²

2020

Basrah J. Agric. Sci.

View full text Add to dashboard Cite

Conventional agriculture created ecological and sociological problems worldwide. Practicing organic agriculture need profitable crop such as lucerne, no tillage to destroy the soil structure, usage of natural symbiotic N2 fixation to support nutrition and reduce damages from climatic changes such as droughts and also to supply animals for food. The efficiency of manure fertilization for the production of forage, seeds and root mass after four years of lucerne cultivation was examined in the field experiment,in addition their effect on the following crop such as oat. The trial was performed in the Institute of Forage Crops, Pleven, Bulgaria on leached chernozem soil subtype without irrigation. The well matured cattle manure in the following doses was used: dose 1 - 20.7 t.ha-1 corresponding to 70 kg N. ha-1 (active substance); dose 2 - 41.5 t. ha-1 corresponding to 140 kg N. ha-1 and dose 3 - 62.1 t.ha-1 corresponding to 210 kg N ha-1. It was found that, the highest organic fertilizer use efficiency (153.16) was found in lucerne for forage at a dose of 20.7 t. ha-1. The largest differences in fertilization efficiency depending on the dose were reported in lucerne for forage (1.9 and 2.8 times). The highest efficiency of manure fertilization for root mass accumulation was reported at a dose of 20.7 t. ha-1. The results showed that lower doses of manure can contribute to obtain a high yield of forage, straw, roots and seeds from lucerne. This has a positive effect on grain and straw and also to the following crop such as oat. Organic farming clearly indicated the advantages for both yields and environmentally friendly agriculture and reduction of environmental stresses.

show abstract

Crop Improvement Through Microbial Biotechnology: A Cross Talk

Cited by 10 publications

References 119 publications

Implications of Seed Vault Storage Strategies for Conservation of Seed Bacterial Microbiomes

Implications of Seed Vault Storage Strategies for Conservation of Seed Bacterial Microbiomes

Salinity stress endurance of the plants with the aid of bacterial genes

Organic Fertilization of Lucerne and the Following Oat Crop in Non-Irrigated Conditions

Contact Info

Product

Resources

About