Cross clade prophylactic and therapeutic efficacy of polyvalent equine immunoglobulin F(ab′)2 against highly pathogenic avian influenza H5N1 in mice

Zhao, Zhongpeng; Fang, Yan; Chen, Zhongwei; Luo, Di; Duan, Yueqiang; Yang, Peng; Zhong, Li; Peng, Daxin; Liu, Xiufan; Wang, Xiliang

doi:10.1016/j.intimp.2011.08.011

Cited by 11 publications

(12 citation statements)

References 33 publications

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“…Sero-neutralising assays and haemagglutination inhibition tests confirmed the potent neutralising abilities of equine polyclonal anti-HPAI H5N1 F(ab ) 2 preparations in mice [145]. In another study, four H5N1 avian influenza equine F(ab ) 2 preparations demonstrated cytopathic effect against cultured Madin-Darby canine kidney (MDCK) cells infected with H5N1 and protected mice against lethal challenge, both given prior (prophylaxis) or post (therapeutic) exposure [146]. These studies concur with earlier mice studies of polyclonal antibodies to seasonal influenza A.…”

Section: Polyclonal Antibodies For Emerging and Neglected Viral Diseasesmentioning

confidence: 82%

Benefits of using heterologous polyclonal antibodies and potential applications to new and undertreated infectious pathogens

Dixit

Herz²,

Dalton

et al. 2016

Vaccine

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Section: Polyclonal Antibodies For Emerging and Neglected Viral Diseasesmentioning

confidence: 82%

Benefits of using heterologous polyclonal antibodies and potential applications to new and undertreated infectious pathogens

Dixit

Herz²,

Dalton

et al. 2016

Vaccine

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“…Equine F(ab′)2 fragments surmount the bottleneck of antibody yield, possess broader antigenic coverage, and avoid the emergence of escape mutants [25,26,27]. Of note, equine antibodies are relatively economical and are readily available.…”

Section: Discussionmentioning

confidence: 99%

Equine Immunoglobulin and Equine Neutralizing F(ab′)2 Protect Mice from West Nile Virus Infection

Wang

Zhao

Wang

et al. 2016

Viruses

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West Nile virus (WNV) is prevalent in Africa, Europe, the Middle East, West Asia, and North America, and causes epidemic encephalitis. To date, no effective therapy for WNV infection has been developed; therefore, there is urgent need to find an efficient method to prevent WNV disease. In this study, we prepared and evaluated the protective efficacy of immune serum IgG and pepsin-digested F(ab′)2 fragments from horses immunized with the WNV virus-like particles (VLP) expressing the WNV M and E proteins. Immune equine F(ab′)2 fragments and immune horse sera efficiently neutralized WNV infection in tissue culture. The passive transfer of equine immune antibodies significantly accelerated the virus clearance in the spleens and brains of WNV infected mice, and reduced mortality. Thus, equine immunoglobulin or equine neutralizing F(ab′)2 passive immunotherapy is a potential strategy for the prophylactic or therapeutic treatment of patients infected with WNV.

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“…For each inoculation, 5 ml of antigen suspension was mixed with an equal volume of Freund′s adjuvant according to the directions and injected into the several sites near the submandibular and inguinal lumph nodes. Two weeks post the last immunization, the horses were bled and the sera were detected for the titer of influenza A H7N9 virus specific antibody by hemagglutination inhibition (HI) assay as previously described [15].…”

Section: Inoculating Horsesmentioning

confidence: 99%

“…The F(ab′) 2 fragments of equine serum IgGs with the HI titer of 1:1024 were prepared by Shanghai Serum Bio-tech Co., Ltd.(Shanghai, China) as previously described [15]. The final F(ab′) 2 products were determined by Lowry method, HPLC respectively and stored at 4°C until use.…”

Section: Preparation Of the F(ab′) 2 Fragments Of Equine Serum Iggsmentioning

confidence: 99%

“…The practice of administering polyclonal immunoglobulins from hyperimmune sera of animal or human origin has a century old history for controlling many viral infections, such as HIV [12], rabies [13] and hepatitis B virus [14]. In our previous studies, we have developed equine anti-SARS-CoV and anti-HPAI H5N1 virus F(ab′)2 fragments, which were shown to effectively neutralize SARS-CoV or H5N1 virus in vitro and in vivo [15,16].…”

Section: Introductionmentioning

confidence: 99%

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Protection from infection with influenza A H7N9 virus in a mouse model by equine neutralizing F(ab′)2

Zhao

Fan

Duan

et al. 2014

International Immunopharmacology

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Influenza A H7N9 virus has demonstrated considerable pandemic potential in China ever since early spring 2013. Until now, there have been no specific medicines to treat influenza A H7N9 virus infected patients. Development of a safe and effective H7N9 therapeutic preparation is urgently needed. To this end, we prepared and evaluated the pepsin-digested F(ab')2 fragments of serum IgGs from the horses inoculated with a inactivated influenza A H7N9 whole virus antigens. The protective effects of the F(ab')2 fragments against H7N9 virus infection were determined in cultured MDCK cells by cytopathic effect (CPE) and evaluated in a BALB/c mouse model by observing death, weight loss and viral load. The in vitro results showed that the F(ab')2 fragments had an HI titer of 1:2048 and a neutralization titer of 1: 31,623. The in vivo assays suggested that 600 U of the preparations could efficiently protect BALB/c mice from a lethal dose of A/Anhui/01/2013 (H7N9) infection even when administered two days post infection. Thus, this highly purified preparation should be a potential candidate for treating severe patients suffering from influenza A H7N9.

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Cross clade prophylactic and therapeutic efficacy of polyvalent equine immunoglobulin F(ab′)2 against highly pathogenic avian influenza H5N1 in mice

Cited by 11 publications

References 33 publications

Benefits of using heterologous polyclonal antibodies and potential applications to new and undertreated infectious pathogens

Benefits of using heterologous polyclonal antibodies and potential applications to new and undertreated infectious pathogens

Equine Immunoglobulin and Equine Neutralizing F(ab′)2 Protect Mice from West Nile Virus Infection

Protection from infection with influenza A H7N9 virus in a mouse model by equine neutralizing F(ab′)2

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