Cross‐linkable graphene oxide embedded nanocomposite hydrogel with enhanced mechanics and cytocompatibility for tissue engineering

Liu, Xifeng; Miller, Arthur K.; Waletzki, Brian E.; Lu, Lichun

doi:10.1002/jbm.a.36322

Cited by 11 publications

(11 citation statements)

References 62 publications

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“…GO nanosheets were oxidized and exfoliated from natural graphite (∼150 μm flakes, Sigma-Aldrich, Milwaukee, WI) through improved Hummers’ method. , Briefly, graphite flakes (3 g) and KMnO 4 (18 g) were added into concentrated H 2 SO 4 /H 3 PO 4 (360 mL/40 mL) under vigorous stirring. The components were reacted at 50 °C under constant stirring for 12 h and then transferred into a glass beaker filled with ice and 3 mL of 30% H 2 O 2 .…”

Section: Methodsmentioning

confidence: 99%

Two-Dimensional Black Phosphorus and Graphene Oxide Nanosheets Synergistically Enhance Cell Proliferation and Osteogenesis on 3D Printed Scaffolds

Liu

Miller

Park

et al. 2019

ACS Appl. Mater. Interfaces

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112

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Two-dimensional (2D) materials have emerged as a new promising research topic for tissue engineering because of their ability to alter the surface properties of tissue scaffolds and thus improve their biocompatibility and cell affinity. Multiple 2D materials, such as graphene and graphene oxide (GO), have been widely reported to enhance cell adhesion and proliferation. Recently, a newly emerged black phosphorus (BP) 2D material has attracted attention in biomedical applications because of its unique mechanical and electrochemical characteristics. In this study, we investigated the synergistic effect of these two types of 2D materials on cell osteogenesis for bone tissue engineering. BP was first wrapped in negatively charged GO nanosheets, which were then adsorbed together onto positively charged poly(propylene fumarate) three-dimensional (3D) scaffolds. The increased surface area provided by GO nanosheets would enhance cell attachment at the initial stage. In addition, slow oxidation of BP nanosheets wrapped within GO layers would generate a continuous release of phosphate, an important osteoblast differentiation facilitator designed to stimulate cell osteogenesis toward the new bone formation. Through the use of 3D confocal imaging, unique interactions between cells and BP nanosheets were observed, including a stretched cell shape and the development of filaments around the BP nanosheets, along with increased cell proliferation when compared with scaffolds incorporating only one of the 2D materials. Furthermore, the biomineralization of 3D scaffolds, as well as cellular osteogenic markers, was all measured and improved on scaffolds with both BP and GO nanosheets. All these results indicate that the incorporation of 2D BP and GO materials could effectively and synergistically stimulate cell proliferation and osteogenesis on 3D tissue scaffolds.

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Section: Methodsmentioning

confidence: 99%

Two-Dimensional Black Phosphorus and Graphene Oxide Nanosheets Synergistically Enhance Cell Proliferation and Osteogenesis on 3D Printed Scaffolds

Liu

Miller

Park

et al. 2019

ACS Appl. Mater. Interfaces

Self Cite

112

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“…Moreover, the functionalization of GDs could also significantly influence the reinforcing effect mainly from two aspects, one of which was that enhanced binding between GDs and matrix might be achieved after the functionalizations, 81,82 and the other of which was that appropriate functionalizations could improve dispersion of GDs in the matrix, thereby getting better structural uniformity. 83,84 For example, Ionita et al 81 prepared porous polyvinyl alcohol Gel-polyvinyl alcohol (Gel-PVA) /GO materials for bone repair by freeze-drying method. Their subsequent results showed that addition of the GO could enhance the compressive strength of the material by 100%.…”

Section: Outstanding Mechanical Propertiesmentioning

confidence: 99%

<p>Applications of Graphene and Its Derivatives in Bone Repair: Advantages for Promoting Bone Formation and Providing Real-Time Detection, Challenges and Future Prospects</p>

Du¹,

Wang

Zhang³

et al. 2020

IJN

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During continuous innovation in the preparation, characterization and application of various bone repair materials for several decades, nanomaterials have exhibited many unique advantages. As a kind of representative two-dimensional nanomaterials, graphene and its derivatives (GDs) such as graphene oxide and reduced graphene oxide have shown promising potential for the application in bone repair based on their excellent mechanical properties, electrical conductivity, large specific surface area (SSA) and atomic structure stability. Herein, we reviewed the updated application of them in bone repair in order to present, as comprehensively, as possible, their specific advantages, challenges and current solutions. Firstly, how their advantages have been utilized in bone repair materials with improved bone formation ability was discussed. Especially, the effects of further functionalization or modification were emphasized. Then, the signaling pathways involved in GDs-induced osteogenic differentiation of stem cells and immunomodulatory mechanism of GDs-induced bone regeneration were discussed. On the other hand, their applications as contrast agents in the field of bone repair were summarized. In addition, we also reviewed the progress and related principles of the effects of GDs parameters on cytotoxicity and residues. At last, the future research was prospected.

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“…Furthermore, the hydrogel is not easily destroyed at high temperatures and has high stability, and its biocompatibility is improved compared to PAM hydrogels. 68 7.2. Carboxymethyl Chitosan/Graphene Oxide/Polyacrylamide Nanocomposite Hydrogel.…”

Section: Some High-performance Hydrogelmentioning

confidence: 99%

Application of Graphene Oxide-Based Hydrogels in Bone Tissue Engineering

Zhihui

Dai

2022

ACS Biomater. Sci. Eng.

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As an important derivative of graphene-based materials, graphene oxide (GO) not only plays an important role not only in optoelectronics and sensing but also in biology due to its unique mechanical, electronic, and optical properties. This article reviews the application of GO-based hydrogels in bone tissue engineering. Whether it is a hydrogel synthesized with natural polymer compounds, synthetic polymer chemicals, bioceramics, bioactive factors, or other materials, the addition of GO can significantly improve various properties of the hydrogel. We also introduce some high-performance GO-based hydrogels in this paper, proposing some insights into materials that may be applied to bone tissue engineering in the future.

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Cross‐linkable graphene oxide embedded nanocomposite hydrogel with enhanced mechanics and cytocompatibility for tissue engineering

Cited by 11 publications

References 62 publications

Two-Dimensional Black Phosphorus and Graphene Oxide Nanosheets Synergistically Enhance Cell Proliferation and Osteogenesis on 3D Printed Scaffolds

Two-Dimensional Black Phosphorus and Graphene Oxide Nanosheets Synergistically Enhance Cell Proliferation and Osteogenesis on 3D Printed Scaffolds

<p>Applications of Graphene and Its Derivatives in Bone Repair: Advantages for Promoting Bone Formation and Providing Real-Time Detection, Challenges and Future Prospects</p>

Application of Graphene Oxide-Based Hydrogels in Bone Tissue Engineering

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