Cross-Linked Gelatine by Modified Dextran as a Potential Bioink Prepared by a Simple and Non-Toxic Process

Musilová, Lenka; Achbergerová, Eva; Vítková, Lenka; Kolarik, Roman; Martinková, Martina; Minařík, Antonín; Mráček, Aleš; Humpolíček, Petr; Pecha, Jiří

doi:10.3390/polym14030391

Cited by 9 publications

(4 citation statements)

References 67 publications

(86 reference statements)

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“…Commonly, dextran-based tissue adhesives are widely applied in drug deliveries [163,164], tissue repair [165,166], and bioprinting [167,168]. By combining other functional polymers, dextran-based adhesives can obtain new functions.…”

Section: Dextranmentioning

confidence: 99%

Recent Advances in the Degradability and Applications of Tissue Adhesives Based on Biodegradable Polymers

Zhu,

Dou,

Zeng

et al. 2024

IJMS

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In clinical practice, tissue adhesives have emerged as an alternative tool for wound treatments due to their advantages in ease of use, rapid application, less pain, and minimal tissue damage. Since most tissue adhesives are designed for internal use or wound treatments, the biodegradation of adhesives is important. To endow tissue adhesives with biodegradability, in the past few decades, various biodegradable polymers, either natural polymers (such as chitosan, hyaluronic acid, gelatin, chondroitin sulfate, starch, sodium alginate, glucans, pectin, functional proteins, and peptides) or synthetic polymers (such as poly(lactic acid), polyurethanes, polycaprolactone, and poly(lactic-co-glycolic acid)), have been utilized to develop novel biodegradable tissue adhesives. Incorporated biodegradable polymers are degraded in vivo with time under specific conditions, leading to the destruction of the structure and the further degradation of tissue adhesives. In this review, we first summarize the strategies of utilizing biodegradable polymers to develop tissue adhesives. Furthermore, we provide a symmetric overview of the biodegradable polymers used for tissue adhesives, with a specific focus on the degradability and applications of these tissue adhesives. Additionally, the challenges and perspectives of biodegradable polymer-based tissue adhesives are discussed. We expect that this review can provide new inspirations for the design of novel biodegradable tissue adhesives for biomedical applications.

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Section: Dextranmentioning

confidence: 99%

Recent Advances in the Degradability and Applications of Tissue Adhesives Based on Biodegradable Polymers

Zhu,

Dou,

Zeng

et al. 2024

IJMS

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“…Composite blends help bridge this gapfor instance, agarose and alginate help alter the stiffness of bioinks, gellan gum increases control over material viscosity, and biodegradable dextran permits the fabrication of structures responsive to cellular enzymatic degradation. [79][80][81][82] Such properties help simulate processes such as cancer cell invasion and macrophagemediated tissue remodeling, wherein the microenvironmental properties FIG. 3.…”

Section: B Biopolymers As Monolithic Bioink Substratesmentioning

confidence: 99%

Design approaches for 3D cell culture and 3D bioprinting platforms

Sreepadmanabh,

Arun,

Bhattacharjee

2024

Biophysics Reviews

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The natural habitat of most cells consists of complex and disordered 3D microenvironments with spatiotemporally dynamic material properties. However, prevalent methods of in vitro culture study cells under poorly biomimetic 2D confinement or homogeneous conditions that often neglect critical topographical cues and mechanical stimuli. It has also become increasingly apparent that cells in a 3D conformation exhibit dramatically altered morphological and phenotypical states. In response, efforts toward designing biomaterial platforms for 3D cell culture have taken centerstage over the past few decades. Herein, we present a broad overview of biomaterials for 3D cell culture and 3D bioprinting, spanning both monolithic and granular systems. We first critically evaluate conventional monolithic hydrogel networks, with an emphasis on specific experimental requirements. Building on this, we document the recent emergence of microgel-based 3D growth media as a promising biomaterial platform enabling interrogation of cells within porous and granular scaffolds. We also explore how jammed microgel systems have been leveraged to spatially design and manipulate cellular structures using 3D bioprinting. The advent of these techniques heralds an unprecedented ability to experimentally model complex physiological niches, with important implications for tissue bioengineering and biomedical applications.

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“…[193,295,296] Musilova et al achieved imine crosslinked bioinks by combining oxidized dextran with gelatin from three different sources. [297] These bioinks displayed strong shearthinning behavior with excellent viscoelastic properties, thereby enabling EBB. No cytotoxicity was observed in any of the gelatinbased bioinks, and fibroblasts cells were distributed homogenously without any disruption of the cell structure.…”

Section: Gelatin Cross-linking Using Imine Bondingmentioning

confidence: 99%

Advances in Gelatin Bioinks to Optimize Bioprinted Cell Functions

Asim

Tabish

Liaqat

et al. 2023

Adv Healthcare Materials

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Gelatin is a widely utilized bioprinting biomaterial due to its cell‐adhesive and enzymatically cleavable properties, which improve cell adhesion and growth. Gelatin is often covalently cross‐linked to stabilize bioprinted structures, yet the covalently cross‐linked matrix is unable to recapitulate the dynamic microenvironment of the natural extracellular matrix (ECM), thereby limiting the functions of bioprinted cells. To some extent, a double network bioink can provide a more ECM‐mimetic, bioprinted niche for cell growth. More recently, gelatin matrices are being designed using reversible cross‐linking methods that can emulate the dynamic mechanical properties of the ECM. This review analyzes the progress in developing gelatin bioink formulations for 3D cell culture, and critically analyzes the bioprinting and cross‐linking techniques, with a focus on strategies to optimize the functions of bioprinted cells. This review discusses new cross‐linking chemistries that recapitulate the viscoelastic, stress‐relaxing microenvironment of the ECM, and enable advanced cell functions, yet are less explored in engineering the gelatin bioink. Finally, this work presents the perspective on the areas of future research and argues that the next generation of gelatin bioinks should be designed by considering cell–matrix interactions, and bioprinted constructs should be validated against currently established 3D cell culture standards to achieve improved therapeutic outcomes.

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Cross-Linked Gelatine by Modified Dextran as a Potential Bioink Prepared by a Simple and Non-Toxic Process

Cited by 9 publications

References 67 publications

Recent Advances in the Degradability and Applications of Tissue Adhesives Based on Biodegradable Polymers

Recent Advances in the Degradability and Applications of Tissue Adhesives Based on Biodegradable Polymers

Design approaches for 3D cell culture and 3D bioprinting platforms

Advances in Gelatin Bioinks to Optimize Bioprinted Cell Functions

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