Cross-Polarized Optical Coherence Tomography System with Unpolarized Light

Hartl, Georg; Parmar, Asha; Sharma, Gargi; Singh, Kanwarpal

doi:10.3390/photonics9020076

Cited by 6 publications

(2 citation statements)

References 23 publications

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“…During imaging, larvae were covered with 0.01% MS-222-containing E3 medium to keep preparations from drying out. Cross-polarized images of the spinal lesion site were acquired using a custom-built CP-OCT system ( 65 ). Light from a broadband supercontinuum laser (YSL Photonics Cat#SC-OEM) was filtered to acquire a spectrum centered at 885 nm with a full width at half maximum of 80 nm.…”

Section: Methodsmentioning

confidence: 99%

Small leucine-rich proteoglycans inhibit CNS regeneration by modifying the structural and mechanical properties of the lesion environment

Kolb

John

et al. 2022

Preprint

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Extracellular matrix (ECM) deposition after central nervous system (CNS) injury leads to inhibitory scarring in mammals, whereas it facilitates axon regeneration in the zebrafish. However, the molecular basis of these different fates is not understood. Here, we identify small leucine-rich proteoglycans (SLRPs) as a causal factor in regeneration failure. We demonstrate that the SLRPs Chondroadherin, Fibromodulin, Lumican, and Prolargin are enriched in human, but not zebrafish, CNS lesions. Targeting SLRPs to the zebrafish injury ECM inhibits axon regeneration and functional recovery. Mechanistically, we find that SLRPs confer structural and mechanical properties to the lesion environment that are adverse to axon growth. Our study reveals SLRPs as previously unknown inhibitory ECM factors in the human CNS that impair axon regeneration by modifying tissue mechanics and structure.

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Section: Methodsmentioning

confidence: 99%

Small leucine-rich proteoglycans inhibit CNS regeneration by modifying the structural and mechanical properties of the lesion environment

Kolb

John

et al. 2022

Preprint

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“…Along this line, OCT systems that can detect the change in the polarization of the light as it interacts with the tissue have been proposed. There are two variants of the OCT systems, namely polarization-sensitive optical coherence tomography (PS-OCT) systems [19][20][21] and cross-polarized optical coherence tomography (CP-OCT) systems [22][23][24][25][26][27][28] which have been demonstrated. CP-OCT systems are simpler than PS-OCT systems because such systems do not require defined polarization of light to study the tissue.…”

Section: Introductionmentioning

confidence: 99%

A Proposal to Perform High Contrast Imaging of Human Palatine Tonsil with Cross Polarized Optical Coherence Tomography

et al. 2022

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The palatine tonsils provide the first line of immune defense against foreign pathogens inhaled or ingested. However, a disruption in the epithelial layer within the tonsil crypts can lead to recurrent acute tonsillitis (RAT). Current imaging techniques suffer from poor resolution and contrast and do not allow a classification of the severity of RAT. We have developed a cross-polarized optical coherence tomography system. The system can detect a change in the polarization of the light after the light-tissue interaction. We demonstrate improved resolution and contrast in tonsil imaging with the developed method. Intensity, as well as retardance images of the excised tonsil tissue, were acquired. Features such as crypt epithelium, lymphoid follicles, and dense connective tissue were observed with improved contrast. Cross polarized optical coherence tomography can be a valuable tool in the clinic to evaluate palatine tonsils as it would allow visualizing common tonsil features without the need for any external contrast agent.

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Small leucine-rich proteoglycans inhibit CNS regeneration by modifying the structural and mechanical properties of the lesion environment

Kolb,

Tsata,

John

et al. 2023

Nat Commun

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Extracellular matrix (ECM) deposition after central nervous system (CNS) injury leads to inhibitory scarring in humans and other mammals, whereas it facilitates axon regeneration in the zebrafish. However, the molecular basis of these different fates is not understood. Here, we identify small leucine-rich proteoglycans (SLRPs) as a contributing factor to regeneration failure in mammals. We demonstrate that the SLRPs chondroadherin, fibromodulin, lumican, and prolargin are enriched in rodent and human but not zebrafish CNS lesions. Targeting SLRPs to the zebrafish injury ECM inhibits axon regeneration and functional recovery. Mechanistically, we find that SLRPs confer mechano-structural properties to the lesion environment that are adverse to axon growth. Our study reveals SLRPs as inhibitory ECM factors that impair axon regeneration by modifying tissue mechanics and structure, and identifies their enrichment as a feature of human brain and spinal cord lesions. These findings imply that SLRPs may be targets for therapeutic strategies to promote CNS regeneration.

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Cross-Polarized Optical Coherence Tomography System with Unpolarized Light

Cited by 6 publications

References 23 publications

Small leucine-rich proteoglycans inhibit CNS regeneration by modifying the structural and mechanical properties of the lesion environment

Small leucine-rich proteoglycans inhibit CNS regeneration by modifying the structural and mechanical properties of the lesion environment

A Proposal to Perform High Contrast Imaging of Human Palatine Tonsil with Cross Polarized Optical Coherence Tomography

Small leucine-rich proteoglycans inhibit CNS regeneration by modifying the structural and mechanical properties of the lesion environment

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