Cross-Reactivity Between HTLV Indeterminate Western Blot and Dengue Virus in Individuals From Rio De Janeiro, Brazil.

Carvalho, S Mf; Pombo‐de‐Oliveira, Maria S.; Thuler, L Cs; Leite, Nícolas Patrícius de Medeiros; Santos, Adriana; Nogueira, R. M.; Miranda, G S; Araujo, Edmundo; Miagostovich, M P; Schatzmayr, Hermann G.

doi:10.1097/00042560-199904010-00197

Cited by 3 publications

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“…The variations verified in the WB test indeterminate results can be partially explained by the different methods used during the period, by the possibility of cross reaction with new varieties of HTLV 12 , cross reaction with other viruses, such as the dengue virus 13 , chickenpox and herpes simplex 14 , severe acute respiratory syndrome virus (SARS) 15 , malaria 14,16 and by the presence of individuals in the initial stages of HTLV infection 17 .…”

Section: Discussionmentioning

confidence: 99%

Decline in prevalence and asymmetric distribution of human T cell lymphotropic virus 1 and 2 in blood donors, State of Minas Gerais, Brazil, 1993 to 2007

Dias-Bastos

Oliveira

Carneiro-Proietti

2010

Rev. Soc. Bras. Med. Trop.

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Introduction: Human T cell lymphotropic virus types 1 and 2 (HTLV-1/2) are endemic in Brazil and are screened for in transfusion services since 1993. This study evaluated the evolution of the prevalence of HTLV-1 and 2 in blood donors of the Hemominas Foundation from 1993 to 2007, and its geographical distribution in State of Minas Gerais, Brazil. Methods: The Hemominas Foundation is a centralized blood center in Minas Gerais, Brazil. The sources of data were the Hemominas Foundation Technical Bulletin and files from the centralized serological laboratory. Donors were tested in the period using enzyme linked immuno sorbent assays (ELISA), followed by Western blot, when repeatedly reactive. The data were analyzed by EPIINFO 6.2 and TABWIN 3.5 softwares. Results: The average seroprevalence in the period 1993-2007 was 0.1%. A steady decline occurred from 0.4% in 1993 to below 0.1% in 2002 and later, with a transient peak of 0.5% in 1994. HTLV reactivity distribution was asymmetrical in the state, with regions of higher prevalence, interspersed with low prevalence areas. Comparison of positive and negative donors verified that increasing age was proportional to virus positivity. Odds ratio for age ranged from 1.43 (30 to 39 years-old) to 3.09 (50 to 65 years-old). Women had a greater chance of being positive (OR-1.64), as previously described. Conclusions: Possible explanations for HTLV-1/2 prevalence decline are the exclusion of positive donors from the donor pool, an increase in repeat donors and ELISA test improvement, with reduction in the number of false positive results.

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Section: Discussionmentioning

confidence: 99%

Decline in prevalence and asymmetric distribution of human T cell lymphotropic virus 1 and 2 in blood donors, State of Minas Gerais, Brazil, 1993 to 2007

Dias-Bastos

Oliveira

Carneiro-Proietti

2010

Rev. Soc. Bras. Med. Trop.

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“…Other presumptive associations of indeterminate profiles have been reported with Plasmodium falciparum [22][23][24][25][26][27], varicella zoster virus or herpes simplex [28][29][30][31], and dengue virus [32]. Moreover, recent seroconversion to positivity for HTLV-1/2 [17], incomplete reactions to molecularly distinct variants of HTLV-1/2 or eventually to another exogenous or endogenous retrovirus [30], and transient infection by HTLV-1 [14], have also been reported.…”

Section: Wb Test Sexmentioning

confidence: 99%

Western blot seroindeterminate individuals for Human T-lymphotropic Virus 1/2 (HTLV-1/2) in Fortaleza (Brazil): a serological and molecular diagnostic and epidemiological approach

Santos

Costa²,

Goubau³

et al. 2003

Braz J Infect Dis

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How to handle Western blot (WB) seroindeterminate individuals for Human T-lymphotropic Virus 1/2 (HTLV-1/2) constitutes a challenge for blood banks and families. We made a cross-sectional study of 191 enzyme linked immunoassay (EIA) reactive individuals from the hematological center (HEMOCE) of Fortaleza (Brazil), examining their serological (WB) and molecular (PCR) diagnosis, and demographic profiles, as well as a possible association of their condition with other infectious pathologies and risk factors. Ethical institutional approval and personal consent were obtained. Out of 191 EIA reactive individuals, 118 were WB seroindeterminate and 73 were seropositive for HTLV-1/2. In the PCR analysis of 41 WB seroindeterminate individuals, 9 (22%) were positive and 32 (78%) were negative for HTLV-1/2. The demographic analysis indicated a trend towards a predominance of males among the seroindeterminate individuals and females in the seropositive ones. The seroindeterminate individuals were younger than the seropositive ones. We did not find any association of these conditions with syphilis, Chagas disease or HIV or hepatitis, and with risk factors such as breast-feeding, blood transfusion, STD (syphilis) and IDU.

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“…The low return rate for the confirmatory test demonstrated the need to reevaluate the current methods of dealing with reactive samples. [13][14][15][16][17]. Regarding the sensitivity and specificity of the HTLV screening kits employed in the present study, all samples positive by ELISA kits were confirmed by PCR, therefore the kits was highly sensitive in detecting HTLV-1 and HTLV-2 antibodies.…”

Section: Discussionmentioning

confidence: 94%

Human T-Lymphotropic Virus-1/2 Detected in Drug Abused Prisoners Imprisoned in Correctional Facilities in Central of Java Indonesia

Prasetyo¹,

Dirgahayu²,

Hudiyono³

et al. 2012

UCMS

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Introduction: Human T cell lymphotropic virus types 1 and 2 (HTLV-1/2) has been believed not circulate in Indonesia yet. This study evaluated the prevalence of HTLV-1 and 2 in drug abused prisoners imprisoned in correctional facilities in Central of Java Indonesia, to track the presentation of HTLV-1/2 in Indonesia. Methods: A cross-sectional study was conducted in two prisons (Kedung Pane Male Prison Semarang and Women Prison Semarang) in Central of Java Indonesia between August 2009 and October 2009. All drug abused prisoners in both prisons were enrolled in this study (n = 148). Questionnaires were retrieved and bloods were tested in the period using enzyme linked immuno sorbent assays. The serological positive results were then confirmed by RT-PCR nested addressed the part of HTLV-1 LTR and HTLV-2 LTR region, respectively. Results: The average seroprevalence in the period was 1.4% (2/148). All positive serological samples were confirmed by nested RT-PCR. Of these, one was HTLV-1 positive and one was HTLV-2 positive. Molecular analysis of positive PCR products revealed that the HTLV-1 isolate had close relationship with HTLV-1 isolated in Japan while the HTLV-2 isolate with that of isolated in USA. Conclusions: HTLV-1 and HTLV-2 were detected in drug abused prisoners imprisoned in correctional facilities in Central of Java Indonesia indicated that these viruses were circulated in Indonesia.

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Cross-Reactivity Between HTLV Indeterminate Western Blot and Dengue Virus in Individuals From Rio De Janeiro, Brazil.

Cited by 3 publications

References 0 publications

Decline in prevalence and asymmetric distribution of human T cell lymphotropic virus 1 and 2 in blood donors, State of Minas Gerais, Brazil, 1993 to 2007

Decline in prevalence and asymmetric distribution of human T cell lymphotropic virus 1 and 2 in blood donors, State of Minas Gerais, Brazil, 1993 to 2007

Western blot seroindeterminate individuals for Human T-lymphotropic Virus 1/2 (HTLV-1/2) in Fortaleza (Brazil): a serological and molecular diagnostic and epidemiological approach

Human T-Lymphotropic Virus-1/2 Detected in Drug Abused Prisoners Imprisoned in Correctional Facilities in Central of Java Indonesia

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