Cross-talk between NOTCH2 and BMP4/SMAD signaling pathways in bovine follicular granulosa cells

Li, Yating; Jing, Jiongjie; Dang, Wenqing; Jia, Kaiqi; Guo, Xiangyu; Kebreab, E.; Lyu, Lihua; Zhao, Junxing

doi:10.1016/j.theriogenology.2022.04.016

Cited by 5 publications

(4 citation statements)

References 43 publications

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“…Members of the TGF -gene family have a key role in bovine physiology, for example, bone morphogenetic protein 1 ( BMP1) contributes in the selection and dominance of follicles by regulating the proliferation and apoptosis of granulosa cells [ 10 ]. Similarly, bone morphogenetic protein/suppressor against decapentaplegic ( BMP4/SMAD ) signaling pathway has been suggested to play a role in regulation of follicular development by regulation granulosa cells in bovines [ 11 ]. Further, the bone morphogenetic protein 4 ( BMP4 ) knockdown in bovines cumulus cells inhibited the proliferation of cumulus cells, apoptosis, and cell cycle arrest [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of TGF-Beta Gene Family in Buffalo to Identify Gene Duplication and Functional Mutations

Saif-ur-Rehman

Hassan

Rehman

et al. 2022

Genes

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The TGF-β superfamily is ubiquitously distributed from invertebrates to vertebrates with diverse cellular functioning such as cell adhesion, motility, proliferation, apoptosis, and differentiation. The present study aimed to characterize the TGF-β gene superfamily in buffalo through evolutionary, structural, and single nucleotide polymorphism (SNPs) analyses to find the functional effect of SNPs in selected genes. We detected 32 TGF-β genes in buffalo genome and all TGF-β proteins exhibited basic nature except INHA, INHBC, MSTN, BMP10, and GDF2, which showed acidic properties. According to aliphatic index, TGF-β proteins were thermostable but unstable in nature. Except for GDF1 and AMH, TGF-β proteins depicted hydrophilic nature. Moreover, all the detected buffalo TGF-β genes showed evolutionary conserved nature. We also identified eight segmental and one tandem duplication event TGF-β gene family in buffalo, and the ratio of Ka/Ks demonstrated that all the duplicated gene pairs were under selective pressure. Comparative amino acid analysis demonstrated higher variation in buffalo TGF-β gene family, as a total of 160 amino acid variations in all the buffalo TGF-β proteins were detected. Mutation analysis revealed that 13 mutations had an overall damaging effect that might have functional consequences on buffalo growth, folliculogenesis, or embryogenesis.

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Section: Introductionmentioning

confidence: 99%

Molecular Characterization of TGF-Beta Gene Family in Buffalo to Identify Gene Duplication and Functional Mutations

Saif-ur-Rehman

Hassan

Rehman

et al. 2022

Genes

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“…Western blotting was performed as previously described ( 18 ). Briefly, the tissues were powdered and added to an ice-cold protein extraction buffer.…”

Section: Methodsmentioning

confidence: 99%

Neonatal vitamin A supplementation improves sheep fertility potential

Li,

Song,

Zhao

et al. 2024

Front. Vet. Sci.

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This study aimed to explore the effects of neonatal vitamin A (VA) supplementation on testis development and spermatogenesis. A total of 32 newborn lambs were intramuscularly injected with corn oil (control group) or corn oil + 2500 IU/kg BW VA (VA group). They were slaughtered and sampled at 3 weeks and 8 months of age to analyze spermatogenesis, cell proliferation, hormone secretion, antioxidant status of the testis, and adult sheep sperm parameters. Compared with the control group, the expression of spermatogonial differentiation-related genes in VA group was up-regulated (P < 0.05). Testis weight, seminiferous tubule diameter, number of spermatogonium and spermatocyte, and sperm density increased significantly in VA group at 8 months of age (P < 0.05). Neonatal VA injection upregulated the expression of the cell proliferation marker PCNA and cell cycle-related genes in the testis (P < 0.05). VA increased the concentrations of testosterone (T), luteinizing hormone (LH), and follicle-stimulating hormone (FSH) in the serum and upregulated steroidogenesis-related genes in the testis (P < 0.05). The antioxidant levels in the VA group were maintained at high levels. The total antioxidant capacity (T-AOC), antioxidant enzyme content and antioxidant-related genes were increased in the testis (P < 0.05). Furthermore, neonatal VA injection activated retinoic acid (RA) signaling to maintain the blood-testosterone barrier (BTB) in the testis of 3-week-old sheep. AMP-activated protein kinase (AMPK) and protein kinase B (AKT) signaling were also modulated in the sheep testis (P < 0.05). Taken together, VA supplementation in newborn rams promotes testis development and spermatogenesis to improve fertility.

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Section: -14mentioning

confidence: 99%

“…In the anterior pituitary, BMPs also aids to the regulation of gonadotrophin production 16 . NOTCH and bone morphogenetic protein (BMP)/SMAD signaling play key regulatory roles in mammalian ovarian development 17 . The mutation Q249R (glutamine→arginine) in the regulatory serine/threonine kinase domain of BMPR1B receptor was identified in Booroola ewes and causes increased fertility 18 .…”

Section: Introductionmentioning

confidence: 99%

Genomics for reproduction in Anas platyrynchos-a novel report

Debnath

Pal

Chakraborty

et al. 2022

Preprint

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Anas platyrynchos (ducks) are reared mostly for egg, which are very nutritious, that fetch better prices, however duck meat possess rich nutrient content. They possess the unique characteristics of disease resistance to the common avian diseases, even asymptomatic to avian influenza, with a scopeto evolve as one of the best poultry species The major limitation encountered is the lowered average egg production as well as higher age at first egg (an indicator for sexual maturity) for the indigenous ducks compared to that of exotic ones. In this current study, we attempt to explore the genes responsible for duck reproduction in terms of sexual maturity,egg production and fertility of the ducks . We had compared the genomic constitution for the Bengal duck with highest egg production with that of non-layer (infertile or sexually immature) ducks. We characterized the genes in indigenous ducks from ovarian tissues, identified important domains for characterized genes for the first time, and studied differential mRNA expression profiling for these genes with respect to layer and non-layer groups. Upregulation was observed for ESR2, DIAPH2, KMT2E, ASCF2 genes for Bengal duck in highest egg producing duck in comparison to non-layer duck, whereas downregulation was observed for KSR1, A2M, BMPR1B, ACVR1. In the next step, we explored the association with the genes which were actually responsible for egg production. Thus, duck may be utilized as a model for studying the molecular aspect of reproduction. Genes upregulated may be utilized for knock in of gene, whereas down regulated genes may be knocked out or knocked down through gene editing technologies for the improvement of reproductive performance of the duck in future. Molecular biomarkers may be developed with these genes for early selection of better reproducing ducks at day of hatch or even earlier.

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Cross-talk between NOTCH2 and BMP4/SMAD signaling pathways in bovine follicular granulosa cells

Cited by 5 publications

References 43 publications

Molecular Characterization of TGF-Beta Gene Family in Buffalo to Identify Gene Duplication and Functional Mutations

Molecular Characterization of TGF-Beta Gene Family in Buffalo to Identify Gene Duplication and Functional Mutations

Neonatal vitamin A supplementation improves sheep fertility potential

Genomics for reproduction in Anas platyrynchos-a novel report

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