Crowding stress induces changes in serum haemolytic and agglutinating activity in the gilthead sea bream Sparus aurata

Tort, L.; Sunyer, J. Oriol; Gómez, E.; Molinero, Amalia

doi:10.1016/0165-2427(95)05502-9

Cited by 130 publications

(84 citation statements)

References 16 publications

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“…The erythrocyte number decreased at 15 minutes of air exposure, and the values were not affected by vitamin C. Similarly, vitamin C did not change the hemoglobin. These results are similar to verified by Tort et al (1996) and Sadler et al (2000) in seabream (Sparus aurata) and Atlantic salmon (Salmo salar) submitted to crowding stress since no changes either in hematocrit or in hemoglobin were observed in these species.…”

supporting

confidence: 76%

“…Decrease in lymphocyte and increase in neutrophil number are frequently reported in stressed fish (Tort et al, 1996;Rotlant et al, 1997;Benfey & Biron, 2000;Iida & Kurogi, 2001). No significant differences were observed in the total number of leukocyte of matrinxã along the samplings and the values obtained (4,9 to 5,8 cell μL -1 x 10 3 ) were higher than those described by Tavares-Dias et al (1999) for the species (2,6 cell μL -1 x 10 3 ).…”

mentioning

confidence: 99%

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Physiological responses of matrinxã (Brycon amazonicus) fed different levels of vitamin C and submitted to air exposure

Abreu

Urbinati

2006

Acta Amaz.

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The role of vitamin C on physiological responses of matrinxã (Brycon amazonicus) submitted to air exposure was analyzed. Nine hundred fish (70.15 g) were distributed in fifteen 500 l boxes (60 fish.box -1 ) and fed five rations (treatments): Control (no vitamin C); T100 (100 mg); T200 (200 mg); T400 (400 mg) and T800 (800 mg of vitamin C kg.ration -1 ). Each ration was offered to fish of three boxes during 60 days before the stress challenge that consisted of exposing fish to air for two minutes. Samplings were carried out for 5, 15, 30 and 60 minutes after the air exposure. Blood was collected for glucose, cortisol, total protein, sodium, chloride, hematocrit, hemoglobin determination, and white and red cell count. Liver was removed for hepatosomatic index (HSI) calculation and glycogen determination. Vitamin C did not affect the levels of cortisol, chloride, total protein, hemoglobin, leukocytes, hepatic glycogen or HSI in air exposed fish. Blood glucose levels elevation observed 60 minutes after the challenge did not depend on the levels of vitamin C, nor did the drop in serum sodium levels verified 60 minutes after stressor. In general, hematocrit did not change by effect of vitamin C but it was lower at 15 and 30 minutes after the challenge. The number of erythrocytes decreased in fish after 5 minute sampling in all treatments, especially at 30 and 60 minutes. The air exposure evoked alterations in stress indicators of matrinxã, and the vitamin C did not alter the responses. KEY WORDSBrycon amazonicus, capture, stress, ascorbyl -polyphosphate. de glicose, cortisol, proteína total, sódio, cloreto, hematócrito, hemoglobina, contagem Respostas fisiológicas de matrinxã (Brycon amazonicus) arraçoados com diferentes níveis de vitamina C e submetidos à exposição aérea RESUMO Este trabalho avaliou a participação da vitamina C nos indicadoras de estresse no matrinxã (Brycon amazonicus), durante exposição aérea. Novecentos peixes (70,15g) foram distribuídos em caixas de cimento (500 l) e alimentados com os tratamentos: Controle (sem vitamina C); T100 (100 mg); T200 (200 mg); T400 (400 mg); T800 (800 mg de vitamina C kg.ração -1 ). Cada ração foi fornecida a peixes de três caixas por 60 dias antes da aplicação do estressor, que consistiu em suspender os peixes mantendo-os no ar por dois minutos. Cinco, 15, 30 e 60 minutos depois, os peixes anestesiados foram amostrados. Foi coletado sangue para determinação PALAVRAS -CHAVEBrycon amazonicus, captura, estresse, ascorbil polifosfato.

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supporting

confidence: 76%

mentioning

confidence: 99%

Physiological responses of matrinxã (Brycon amazonicus) fed different levels of vitamin C and submitted to air exposure

Abreu

Urbinati

2006

Acta Amaz.

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“…In the same species, chronic stress induced by crowding caused immunodepression, as suggested by decreases in haemagglutination titre observed after 9 days [72].…”

Section: Discussionmentioning

confidence: 99%

Haematological and Immunological Responses in Juvenile Sea Bass (Dicentrarchus labrax L.) After Short-Term Acute Stress

Maricchiolo¹,

Caruso²,

Genovese³

2008

TOFISHSJ

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Abstract:The physiological effects of acute stress (induced by an intraperitoneal injection) on the haematocrit, haemoglobin, serum cortisol and glucose, haemagglutinins and lysozyme were investigated in juvenile sea bass Dicentrarchus labrax. A total of 40 fish were subjected to an intraperitoneal injection. Eight fish were sampled at 0, 2, 4, 24 and 48 h after acute stress and compared to unstressed fish (controls) sacrificed at the same times. Intraperitoneal injection resulted in significant changes in cortisol and glucose 4 and 2 hours after exposure to stress, respectively, but not in haematocrit and haemoglobin levels. No significant differences between stressed and control fish were recorded for lysozyme content and haemagglutinating activity throughout the experiment. In juvenile sea bass acute stress caused by intraperitoneal injection was mostly associated with the increase in common stress indicators (cortisol and glucose), while no clear response in terms of non-specific immune parameters was evidenced.

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“…Reproducible purification yields and analysis of serum by Western blot suggest that MsaFBP32 is a constitutively expressed protein, and its basal levels of MsaFBP32 are relatively high under the standard husbandry conditions. In aquaculture settings, confinement may be responsible for a "basal" stress level (68), and this may result in the apparently constitutive expression of innate immune recognition factors such as MsaFBP32.…”

Section: Discussionmentioning

confidence: 99%

Characterization of a Binary Tandem Domain F-type Lectin from Striped Bass (Morone saxatilis)

Odom

Vasta

2006

Journal of Biological Chemistry

137

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Among other functions, lectins play an important role in the innate immune response of vertebrates and invertebrates by recognizing exposed glycans on the surface of potential pathogens. Despite the typically weak interaction of lectin domains with their carbohydrate ligands, they usually achieve high avidity through oligomeric structures or by the presence of tandem carbohydratebinding domains along the polypeptide. The recently described structure of the fucose-binding European eel agglutinin revealed a novel lectin fold (the "F-type" fold), which is shared with other carbohydrate-binding proteins and apparently unrelated proteins from prokaryotes to vertebrates, and a unique fucose-binding sequence motif. Here we described the biochemical and molecular characterization of a unique fucose-binding lectin (MsaFBP32) isolated from serum of the striped bass (Morone saxatilis), composed of two tandem domains that exhibit the eel carbohydrate recognition sequence motif, which we designate F-type. We also described a novel lectin family ("F-type") constituted by a large number of proteins exhibiting greater multiples of the F-type motif, either tandemly arrayed or in mosaic combinations with other domains, including a putative transmembrane receptor, that suggests an extensive functional diversification of this lectin family. Among the tandem lectins, MsaFBP32 and other tandem binary homologues appear unique in that although their N-terminal domain shows close similarity to the fucose recognition domain of the eel agglutinin, their C-terminal domain exhibits changes that potentially could confer a distinct specificity for fucosylated ligands. In contrast with the amniotes, in which the F-type lectins appear conspicuously absent, the widespread gene duplication in the teleost fish suggests these F-type lectins acquired increasing evolutionary value within this taxon.

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Crowding stress induces changes in serum haemolytic and agglutinating activity in the gilthead sea bream Sparus aurata

Cited by 130 publications

References 16 publications

Physiological responses of matrinxã (Brycon amazonicus) fed different levels of vitamin C and submitted to air exposure

Physiological responses of matrinxã (Brycon amazonicus) fed different levels of vitamin C and submitted to air exposure

Haematological and Immunological Responses in Juvenile Sea Bass (Dicentrarchus labrax L.) After Short-Term Acute Stress

Characterization of a Binary Tandem Domain F-type Lectin from Striped Bass (Morone saxatilis)

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