CRP and IL-1B Gene Polymorphisms and CRP in Blood in Periodontal Disease

Auerkari, Elza Ibrahim; Suhartono, AW; Djamal, NZ; Verisqa, F; Suryandari, DA; Kusdhany, Lindawati S.; Masulili, Slc; Talbot, Chris J.

doi:10.2174/1874210601307010088

Cited by 18 publications

(12 citation statements)

References 15 publications

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“…Eight papers classified as poor, 1 as good, and the remaining 43 papers were of moderate quality (Figure ). The lowest score reached by a paper was 3, while the best score was 13 . The items most frequently deemed inadequate involved the handling of confounders, especially oral hygiene habits, socioeconomic status, presence of specific periodontal pathogens, and stress.…”

Section: Resultsmentioning

confidence: 99%

Quality of methods and reporting in association studies of chronic periodontitis and IL1A−889 and IL1B+3953/4 SNPs: A systematic review

Citterio

Romano

Ferrarotti

et al. 2019

J of Periodontal Research

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Objective The aim of this systematic review was to evaluate the quality of reporting and methodology in genetic association studies between IL1A −889 and IL1B +3954 polymorphisms and chronic periodontitis. Background Evidence provided by periodontal research on genetic risk factors is of uttermost importance in clinical practice as a possible diagnostic and prognostic tool for periodontitis. Inadequate reporting of results as well as high risk of bias due to methodological inconsistency hampers the integration of evidence in terms of clinical applicability. Methods This review includes case‐control studies in humans published between 1997 and July 2017. Searching was conducted through MEDLINE, EMBASE, and search handing. Specific scoring systems have been developed to evaluate the quality of methods and reporting. Each article was scored according to its adequacy, and then, the total number and the percentage of items positively qualified for both methods and reporting were calculated. The quality of methods in studies scoring 0‐6, 7‐12, and 13‐16 was, respectively, considered poor, moderate, and good. For reporting, scores of 0‐9, 10‐18, and 19‐26 were deemed of poor, moderate, and good quality, respectively. Pearson's correlation coefficient was calculated to explore the correlation between the year of publication and the quality in terms of methods and reporting. Results From the 531 screened studies, 52 met the inclusion criteria and were thus included in the study. The quality of methods and reporting of published genetic association papers on IL1 and chronic periodontitis is moderate. On a scale from 0 to 16, the mean score for methods of the reviewed studies was 8.19 ± 1.93. The items more frequently considered inadequate concerned the handling of confounders in statistical analysis, especially oral hygiene habits, socioeconomic status, subgingival colonization of specific periodontal pathogens, and stress. A significant positive correlation was found between the year of publication and the quality scores in terms of method (r = 0.401, P = 0.003). In terms of reporting, the mean score was 14.83 ± 3.04 on a scale from 0 to 26 and it was considered overall moderate. No statistically significant correlation was found between the year of publication and the quality of reporting (P = 0.266). Conclusions The association between IL1A −889 and IL1B +3954 polymorphisms and chronic periodontitis is questionable due to methodological inconsistency. Evidence arising from meta‐analysis is unreliable due to high risk of bias and moderate quality in terms of reporting.

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Section: Resultsmentioning

confidence: 99%

Quality of methods and reporting in association studies of chronic periodontitis and IL1A−889 and IL1B+3953/4 SNPs: A systematic review

Citterio

Romano

Ferrarotti

et al. 2019

J of Periodontal Research

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“…These molecules are necessary to bind leucocytes in the endothelium layer at the early stage of atherosclerosis. 16 These bacteria will produce a leucotoxin that induces degranulation and lysis in human neutrophils, caspase-1 activation, and abundant secretion of cytokine from human macrophages, promoting a tissue destruction including loss of alveolar bone. 17 Therefore, theoretical based revealed that the level of CRP by the induction of bacterial leucotoxin are supposed largely expressed in severe periodontitis group.…”

Section: Methodsmentioning

confidence: 99%

Serum C-reactive protein and C-reactive gene (-717C>T) polymorphism are not associated with periodontitis in Indonesian male patients

Suhartono

Sulijaya

Djamal

et al. 2015

Dent. J. (Majalah Kedokteran Gigi)

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Background: Periodontitis is an inflammatory disease caused by periodontal pathogens and influenced by multiple risk factors such as genetics, smoking habit, age and systemic diseases. The inflammatory cascade is characterized by the release of C-reactive protein (CRP). Periodontitis has been reported to have plausible links to increased level of CRP, which in turn has been associated to elevated risk of cardiovascular disease (CVD). Purpose: The purpose of this study was t o investigate the relationship amongst the severity of periodontitis, CRP level in blood and CRP (-717 C>T) gene polymorphism in male Indonesian smokers and non-smokers. Method: The severity of periodontitis was assessed for 97 consenting male Indonesian smokers and non-smokers. The CRP level of the subjects was determined by using immuno-turbidimetric assay performed in PARAHITA Diagnostic Center Laboratory ISO 9001: 2000 Cert No. 15225/2. The rate of CRP (-717C>T) gene polymorphism was determined by using PCR-RFLP in Oral Biology Laboratory, Faculty of Dentistry, Universitas Indonesia. Result: The results suggest that the CRP protein level is not significantly associated with the tested CRP gene polymorphism (p>0.05). Also, while the severity of periodontitis increased significantly with subject age, the CRP level in blood serum was not significantly related to the severity of periodontitis. The genotypes of the tested polymorphism did not show significant association with the severity of periodontitis either in smokers or in the combined population including smokers and non-smokers. The results naturally do not exclude such associations, but suggest that to discern the differences the sample size must be considerably increased. Conclusion: The CRP (-717C>T) gene polymorphism and CRP level in blood serum were not found to be associated with the severity of periodontitis in male smokers or in the combined population of smokers and non-smokers.

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“…To identify ENAM C2452T polymorphism, we performed DNA isolation as described by Auerkari et al [15][16][17] and genotyping procedures as described by Ouryouji et al [14]. Each 20-µL polymerase chain reaction (PCR) mixture contained 10 µL of ready-to-use master mix (Bioline, London, UK), 0.4 µL of forward (5'-AGG ATT TTT ATT ACA GTG AAT TTT ACC CAT GGG GCC-3') and reverse primers (3'-CCT TAA GGA CCC TGG TGT CCT ATG TTC-5'), and 20 µg of genomic DNA.…”

Section: Dna Isolation and Genotypingmentioning

confidence: 99%

Association of Enam C2452t Polymorphism With High Rates of Caries Occurrence in an Indonesian Population

Nicoline

Partakusuma

Joenoes³

et al. 2020

Int J App Pharm

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Objective: Tooth decay or the caries process is a common dental problem that affects millions of people worldwide. Many risk factors are modifiable,while others are not (e.g., genetic factors). Polymorphism of the enamelin (ENAM) gene, which is required to ensure production of an essentialprotein for enamel development, may pose as a risk factor for the caries process. This study sought to investigate the possibility of ENAM C2452Tpolymorphism acting as a risk factor in the caries process.Methods: The polymerase chain reaction–restriction fragment length polymorphism method was employed to evaluate DNA samples taken from 95subjects with a high caries prevalence and 89 control subjects for ENAM C2452T polymorphism.Results: Based on Chi-squared tests, there were significant genotype and allele distribution differences between the group with a high cariesprevalence and the control group (p=0.005 and p=0.007). Polymorphism in this context may, therefore, serve as a risk factor for caries onset andprogression (OR: 3.62).Conclusion: ENAM C2452T polymorphism is related to the caries process and may constitute a risk factor.

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CRP and IL-1B Gene Polymorphisms and CRP in Blood in Periodontal Disease

Cited by 18 publications

References 15 publications

Quality of methods and reporting in association studies of chronic periodontitis and IL1A−889 and IL1B+3953/4 SNPs: A systematic review

Quality of methods and reporting in association studies of chronic periodontitis and IL1A−889 and IL1B+3953/4 SNPs: A systematic review

Serum C-reactive protein and C-reactive gene (-717C>T) polymorphism are not associated with periodontitis in Indonesian male patients

Association of Enam C2452t Polymorphism With High Rates of Caries Occurrence in an Indonesian Population

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