2021
DOI: 10.21203/rs.3.rs-864554/v1
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CRP Detection Using Gold Nanosensors – Effect of Layer Thickness and Immobilization Chemistry

Abstract: The immobilization of a capture molecule represents a crucial step for effective usage of gold nanoparticles in localized surface plasmon resonance (LSPR)-based bioanalytics. Depending on the immobilization method used, the resulting capture layer is of varying thickness. Thus, the target binding event takes place in different distances to the gold surface. Using the example of a C-reactive protein (CRP) immunoassay, different immobilization methods were tested and investigated with regard to their resulting t… Show more

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