Crude glycerol negatively affects rabbit spermatozoa motility in vitro

Paál, Dušan; Strejcek, Frantisek; Slanina, Tomáš; Kováčová, Renáta; Lukáč, Norbert; Qoja, A. O.; Ondruška, Ľubomír; Formicki, Grzegorz; Tvrdá, Eva; Massányi, Péter

doi:10.12681/jhvms.15642

Cited by 2 publications

(1 citation statement)

References 24 publications

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“…The results showed that 5% glycerol slightly reduced total motility, while 10%, 15%, and 20% glycerol significantly decreased total overall motility at all time points. Similarly, increasing the concentration of DMSO improved sperm motility after thawing, but it caused acrosome damage to a large extent and reduced pregnancy rates with increasing concentrations (Paal et al, 2016). Overall, the study confirms that an optimal concentration of glycerol or DMSO can improve rabbit sperm quality and that the findings are consistent with previous research.…”

Section: Discussionmentioning

confidence: 99%

Cryopreservation of local black rabbit spermatozoa in the Mekong delta region of Vietnam: Evaluating cryoprotectant effectiveness

Tran,

Chiem,

Tran

et al. 2023

Reprod Domestic Animals

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Rabbit production is well developed in the Mekong Delta and Vietnam, with increased consumption of rabbit meat in both urban and rural areas. Consequently, meat rabbit breeds have become popular in almost all provinces in Vietnam, resulting in a decline in the population of Local Black rabbits over the years. To support breeding programs, there is a crucial need to develop a cryopreservation method. The objective of this study was to develop sperm cryopreservation protocols and determine the optimal cryoprotectant for Local Black rabbit spermatozoa. Rabbit spermatozoa were obtained and diluted with TCG‐EggYolk freezing medium containing 5%, 8%, and 10% glycerol or DMSO in a 1:1 ratio. Glycerol and DMSO‐free mediums served as the controls. Samples were put into 0.5‐mL straws, cooled to 15°C and 5°C, then placed on nitrogen vapour for 15 min and directly immersed in liquid nitrogen at −196°C. Samples were thawed at 37°C for 60 s after being frozen for 72 h Sperm quality measures, such as viability, motility, and membrane integrity, were assessed both before and after freezing. The results showed that the average viability, average motility, and average membrane integrity were 61.9%, 53.9%, and 42.9%, respectively, after thawing with 5% glycerol, which was higher than the two higher concentrations of glycerol (8% and 10%) (p < .05). Similarly, with 8% DMSO, the average viability, average motility, and membrane integrity were 59.2%, 51.0%, and 41.8%, respectively, which were higher than the two concentrations of DMSO (5% and 10%) (p < 0.05). Therefore, the cryopreservation of local black rabbit sperm with 5% glycerol or DMSO 8% resulted in high sperm quality, but considering the practical aspect of minimizing the volume of the preservative, a concentration of 5% glycerol may be the best option.

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Section: Discussionmentioning

confidence: 99%

Cryopreservation of local black rabbit spermatozoa in the Mekong delta region of Vietnam: Evaluating cryoprotectant effectiveness

Tran,

Chiem,

Tran

et al. 2023

Reprod Domestic Animals

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Viscum Album Pini Effect on Rabbit Spermatozoa Motility, Viability, Membrane and Acrosome Integrity in Vitro

Halo¹

2019

J microb biotech food sci

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Mistletoe (Viscum album L.) was previously described as an effective additive in the therapy of patients with cancer after medical therapy of the primary neoplasia. The defence activity and also direct cytotoxic stimulation was found to be upstanding for the anti-carcinogenic action. The main target of this study was to detect the effect of Viscum album pini L. at various concentration 1.0 (PA); 0.66 (PB); 0.33 (PC); 0.1 (PD); 0.066 (PE) and 0.033 (PF) mg/mL on the fine rabbit spermatozoa motility parameters at various time periods (0; 1; 2 and 3 h) during the cultivation in vitro. The CASA methodology was applied to estimate the spermatozoa motility parameters. The spermatozoa motility at the beginning of the experiment (Time 0) found decreased rate for examined doses of Viscum album pini compared to the control, but the statistical significant reduction was observed (p

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Crude glycerol negatively affects rabbit spermatozoa motility in vitro

Cited by 2 publications

References 24 publications

Cryopreservation of local black rabbit spermatozoa in the Mekong delta region of Vietnam: Evaluating cryoprotectant effectiveness

Cryopreservation of local black rabbit spermatozoa in the Mekong delta region of Vietnam: Evaluating cryoprotectant effectiveness

Viscum Album Pini Effect on Rabbit Spermatozoa Motility, Viability, Membrane and Acrosome Integrity in Vitro

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