Crude protein from spirulina increases the viability of CCD‑986sk cells via the EGFR/MAPK signaling pathway

Liu, Ping; Lee, Min‐Kyeong; Choi, Jeong‐Heui; Choi, Youn‐Hee; Nam, Taek‐Jeong

doi:10.3892/ijmm.2018.4025

Cited by 8 publications

(15 citation statements)

References 46 publications

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“…It has been reported that only phosphorylated ERK1/2 is active (42). In a previous study, it was demonstrated that SPcP increased the phosphorylation level of ERK1/2 (27). Thus, the phosphorylation level of ERK1/2 in the granulation tissue of c57BL/6 mice was determined in the present study.…”

Section: Discussionmentioning

confidence: 54%

“…In particular, type I collagen is the most prevalent of the fibril-forming collagens (48). According to previous studies, it has been found that SPcP promotes the secretion of collagen in ccd-986sk cells (27). In the present study, the expression level of type I collagen was determined in granulation tissue of c57BL/6 mice.…”

Section: Discussionmentioning

confidence: 60%

“…SPCP enhances wound repair via the ERK signaling pathway in C57BL/6 mice. According to previous results in ccd-986sk cells (27), it is known that the EGFR/ERK signaling pathway is involved in the SPcP-induced proliferation and migration of ccd-986sk cells. Thus, the effect of SPcP on the phosphorylation level of ERK was determined by western blot analysis.…”

Section: Effect Of 9 Days Of Treatment With Spcp On Lipid Peroxide Anmentioning

confidence: 74%

“…Moreover, spirulina has been reported to exhibit pharmaceutical potential due to its anti-inflammatory, antioxidant and anticancer properties (25,26). Recently, the promoting effects of spirulina protein (SPcP) on the activity of ccd-986sk human fibroblasts were demonstrated (27). Furthermore, SPcP was shown to improve collagen formation in ccd-986sk cells and to decrease the activity of elastase, which plays an important positive role in wound repair (27).…”

Section: Spirulina Protein Promotes Skin Wound Repair In a Mouse Modementioning

confidence: 99%

“…Recently, the promoting effects of spirulina protein (SPcP) on the activity of ccd-986sk human fibroblasts were demonstrated (27). Furthermore, SPcP was shown to improve collagen formation in ccd-986sk cells and to decrease the activity of elastase, which plays an important positive role in wound repair (27). In addition, the migration and proliferation of ccd-986sk cells have been shown to be promoted by SPcP via phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway (28).…”

Section: Spirulina Protein Promotes Skin Wound Repair In a Mouse Modementioning

confidence: 99%

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Spirulina protein promotes skin wound repair in a mouse model of full-thickness dermal excisional wound

et al. 2020

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The skin protects body from environmental damage. Skin wounds lead to microbial infection and harmful agent injury. Thus, wound repair is crucial for the recovery of the normal function of skin tissue. The present study investigated the promoting effects of spirulina protein (SPcP) in mice on skin wound repair and also aimed to elucidate the potential underlying mechanisms. The results revealed that SPcP promoted the skin wound repair in a mouse model of full-thickness excisional wounds. SPcP induced an increase in the expression level of α-smooth muscle actin (α-SMA). The activities of superoxide dismutase (SOd) and catalase (cAT) were enhanced by SPcP treatment in the granulation tissue. In addition, SPcP decreased the level of malondialdehyde (MdA) in the granulation tissue. Western blot analysis revealed that SPcP enhanced the phosphorylation and activation of protein kinase B (Akt) and extracellular signal-regulated kinase (ERK). Moreover, the expression level of transforming growth factor β1 (TGF-β1) was increased in the SPcP-treated groups. The phosphorylation level of Smad2 was also increased by treatment of SPcP. Furthermore, SPcP promoted the expression of collagen in the granulation tissue. Taken together, these findings indicate that SPCP exerts a promoting effect on skin wound repair. The Akt, ERK and TGF-β1 signaling pathways are involved in this process.

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Section: Discussionmentioning

confidence: 54%

Section: Discussionmentioning

confidence: 60%

Section: Effect Of 9 Days Of Treatment With Spcp On Lipid Peroxide Anmentioning

confidence: 74%

Section: Spirulina Protein Promotes Skin Wound Repair In a Mouse Modementioning

confidence: 99%

Section: Spirulina Protein Promotes Skin Wound Repair In a Mouse Modementioning

confidence: 99%

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Spirulina protein promotes skin wound repair in a mouse model of full-thickness dermal excisional wound

et al. 2020

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Potential application of Spirulinain dermatology

Ikeda

Sydney

2022

J of Cosmetic Dermatology

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Spirulinais an edible cyanobacterium known worldwide for its high nutritional value, and the interest in its biological activity and bioactive compounds, such as pigments, phenolics, peptides, and lipids, has been increasing. Due to the variety of its properties, the potential application of Spirulina in health is wide, including dermatological area. In a context of increasing demand for natural products and actives in cosmetics, this microorganism becomes a great source. This article aims to review what has already been reported in literature about the potential effects of Spirulina or its isolated compounds in skin, for either aesthetic or clinical purposes. In many studies, Spirulina and its components show a good influence in proliferation of dermal fibroblasts and keratinocytes, extracellular matrix, and collagen production, as well as exerting antioxidant and anti‐inflammatory action. Thus, they promote a healthy environment for skin's cells and structure, cooperating for the highlighted anti‐aging, photoprotection, and wound‐healing effects. Some compounds of the cyanobacterium also exert a lighting property through tyrosinase inhibition. Its antimicrobial action can also be advantageous to skin contributing to anti‐acne, antibiofilm, and anti‐herpes effects. In face of many attributes and due to its rich composition, Spirulina presents multi‐benefits and shows an improvement in the general aspect of skin. However, some applications are still in need of studying and more clinical evidence is necessary.

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Wound Healing Potential of Spirulina Protein on CCD-986sk Cells

et al. 2019

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Wound healing is a dynamic and complex process. The proliferation and migration of dermal fibroblasts are crucial for wound healing. Recent studies have indicated that the extracts from Spirulina platensis have a positive potential for wound healing. However, its underlying mechanism is not fully understood. Our previous study showed that spirulina crude protein (SPCP) promoted the viability of human dermal fibroblast cell line (CCD-986sk cells). In this study, we further investigated the wound healing effect and corresponding mechanisms of SPCP on CCD-986sk cells. Bromodeoxyuridine (BrdU) assay showed that SPCP promoted the proliferation of CCD-986sk cells. The wound healing assay showed that SPCP promoted the migration of CCD-986sk cells. Furthermore, cell cycle analysis demonstrated that SPCP promoted CCD-986sk cells to enter S and G2/M phases from G0/G1 phase. Western blot results showed that SPCP significantly upregulated the expression of cyclin D1, cyclin E, cyclin-dependent kinase 2 (Cdk2), cyclin-dependent kinase 4 (Cdk4), and cyclin-dependent kinase 6 (Cdk6), as well as inhibited the expression of CDK inhibitors p21 and p27 in CCD-986sk cells. In the meanwhile, SPCP promoted the phosphorylation and activation of phosphoinositide 3-kinase (PI3K) and protein kinase B (Akt). However, the phosphorylation of Akt was significantly blocked by PI3K inhibitor (LY294002), which in turn reduced the SPCP-induced proliferation and migration of CCD-986sk cells. Therefore, the results presenting in this study suggested that SPCP can promote the proliferation and migration of CCD-986sk cells; the PI3K/Akt signaling pathway play a positive and important role in these processes.

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Crude protein from spirulina increases the viability of CCD‑986sk cells via the EGFR/MAPK signaling pathway

Cited by 8 publications

References 46 publications

Spirulina protein promotes skin wound repair in a mouse model of full-thickness dermal excisional wound

Spirulina protein promotes skin wound repair in a mouse model of full-thickness dermal excisional wound

Potential application of Spirulinain dermatology

Wound Healing Potential of Spirulina Protein on CCD-986sk Cells

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