Cryo-EM Structure of raiA ncRNA From Clostridium Reveals a New RNA 3D Fold

Badepally, Nagendar Goud; de Moura, Tales Rocha; Purta, Elżbieta; Baulin, Eugene F.; Bujnicki, Janusz M.

doi:10.1016/j.jmb.2024.168833

Journal of Molecular Biology

2024

DOI: 10.1016/j.jmb.2024.168833

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Cryo-EM Structure of raiA ncRNA From Clostridium Reveals a New RNA 3D Fold

Nagendar Goud Badepally,

Tales Rocha de Moura,

Elżbieta Purta

et al.

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2024

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Naturally ornate RNA-only complexes revealed by cryo-EM

Kretsch,

Wu,

Shabalina

et al. 2024

Preprint

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Myriad families of natural RNAs have been proposed, but not yet experimentally shown, to form biologically important structures. Here we report three-dimensional structures of three large ornate bacterial RNAs using cryogenic electron microscopy at resolutions of 2.9-3.1 Angstrom. Without precedent among previously characterized natural RNA molecules, Giant, Ornate, Lake- and Lactobacillales-Derived (GOLLD), Rumen-Originating, Ornate, Large (ROOL), and Ornate Large Extremophilic (OLE) RNAs form homo-oligomeric complexes whose stoichiometries are retained at concentrations lower than expected in the cell. OLE RNA forms a dimeric complex with long co-axial tubes spanning two monomers. Both GOLLD and ROOL form distinct RNA-only multimeric nanocages with diameters larger than the ribosome. Extensive intra- and intermolecular A-minor interactions, kissing loops, an unusual A-A helix, and other interactions stabilize the three complexes. Sequence covariation analysis of these large RNAs reveals evolutionary conservation of intermolecular interactions, supporting the biological importance of large, ornate RNA quaternary structures that can assemble without any involvement of proteins.

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Naturally ornate RNA-only complexes revealed by cryo-EM

Kretsch,

Wu,

Shabalina

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

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Cryo-EM Structure of raiA ncRNA From Clostridium Reveals a New RNA 3D Fold

Cited by 1 publication

References 54 publications

Naturally ornate RNA-only complexes revealed by cryo-EM

Naturally ornate RNA-only complexes revealed by cryo-EM

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