Cryogels-versatile tools in bioseparation

Ertürk, Gizem; Mattìasson, Bo

doi:10.1016/j.chroma.2014.05.055

Cited by 162 publications

(91 citation statements)

References 125 publications

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“…[25,26] These unique properties of cryogels transform them to a preferred carrier for biomolecules and make them a good chromatographic support material for bioseparation processes. [35] In this presented work, poly(MMA-GMA) cryogels were prepared by using monomers of MMA and GMA with cryopolymerization technique, and used as support material for amyloglucosidase immobilization. A brief schematic presentation for the synthesis of cryogel is demonstrated in Fig 1(A).…”

Section: Properties Of the Cryogel Matrixmentioning

confidence: 99%

Immobilization of amyloglucosidase onto macroporous cryogels for continuous glucose production from starch

Uygun

Akduman

Ergönül

et al. 2015

Journal of Biomaterials Science, Polymer Edition

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Poly(methyl methacrylate-glycidyl methacrylate) [Poly(MMA-GMA)] cryogels were synthesized using monomers of methylmethacrylic acid and epoxy group bearing GMA via radical cryopolymerization technique. Synthesized cryogels were used for the immobilization of amyloglucosidase to the cryogel surface using epoxy chemistry. Characterizations of the free and immobilized amyloglucosidase were carried out by comparing the optimum and kinetic parameters of enzymes. For this, pH and temperature profiles of free and immobilized preparation were studied and, it was found that, optimum pH of enzyme was not change upon immobilization (pH 5.0), while optimum temperature of the enzyme shifted 10 °C to warmer region after immobilization (optimum temperatures for free and immobilized enzyme were 55 and 65 °C, respectively). Kinetic parameters of free and immobilized enzyme were also investigated and Km values of free and immobilized amyloglucosidase were found to be 2.743 and 0.865 mg/mL, respectively. Vmax of immobilized amyloglucosidase was found to be (0.496 µmol/min) about four times less than that of free enzyme (2.020 µmol/min). Storage and operational stabilities of immobilized amyloglucosidase were also studied and it was showed that immobilized preparation had much more stability than free preparation. In the present work, amyloglucosidase immobilized poly(MMA-GMA) cryogels were used for continuous glucose syrup production from starch for the first time. Efficiency of immobilized enzyme was investigated and released amount of glucose was found to be 2.54 mg/mL at the end of the 5 min of hydrolysis. The results indicate that the epoxy functionalized cryogels offer a good alternative for amyloglucosidase immobilization applications with increased operational and thermal stability, and reusability. Also, these cryogels can be used for immobilization of other industrially valuable enzymes beyond amyloglucosidase.

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Section: Properties Of the Cryogel Matrixmentioning

confidence: 99%

Immobilization of amyloglucosidase onto macroporous cryogels for continuous glucose production from starch

Uygun

Akduman

Ergönül

et al. 2015

Journal of Biomaterials Science, Polymer Edition

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“…The important parameters affecting the cryogel characteristics, such as pore size distribution and wall thickness, can be controlled by variation of the monomer concentration, pH, ionic strength, amounts of water and organic co‐solvents, cooling rate and polymerization conditions , . It has been reported that functional monomers based on N ‐methacrylamide and acrylamides form cryogel polymers due to their strong ability to form strong polar, hydrogen, coordination bonds and van der Waals forces in cryogel matrices . Moreover, the functional groups of crosslinkers used to prepare cryogels enhance the mechanical properties, swelling characteristics and the response capability of cryogels to the surrounding environments.…”

Section: Introductionmentioning

confidence: 99%

Removal of organic water pollutant using magnetite nanomaterials embedded with ionic copolymers of 2‐acrylamido‐2‐methylpropane sodium sulfonate cryogels

Al‐Hussain

Ezzat

Gaffer

et al. 2017

Polymer International

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Magnetite cryogel composites as macroporous crosslinked matrices have received wide attention and attract much interest in the water purification and desalination industry. They can be used to produce effective adsorbents with high adsorption rate, capacity and desorption for water pollutants. In this work, the incorporation of magnetite nanoparticles into cryogels by the in situ method is proposed to increase the dispersion of nanoparticles in the gel composites and to produce effective magnetic materials with high adsorption capacities. Ionic sodium‐2‐acrylamido‐2‐methylpropane sulfonate (Na‐AMPS) monomer was selected to prepare cryogels as the homopolymer or copolymers with 2‐hydroxyethyl methacrylate (HEMA) or N‐vinyl pyrrolidone (VP) by the crosslinking polymerization technique in the frozen state. Magnetite nanoparticles were introduced into the cryogel by the in situ co‐precipitation method after introducing iron cations into the cryogel networks. The surface morphologies, crystal structure, magnetite content, thermal stability and magnetic properties were determined for the cryogels and their magnetite composites. The magnetite cryogel composites show significantly enhanced methylene blue dye removal in short times with higher adsorption efficiencies and good regeneration to form an effective adsorbent for water treatment. © 2017 Society of Chemical Industry

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“…Commercially available mixed‐mode sorbents have been used to extract β‐agonists but because of their particulate structure, clogging can occur, especially when used with animal feed, and they are also expensive . To overcome this drawback, a composite SPE sorbent that can incorporate an adsorbent into a highly porous structural material, such as a cryogel, would be an interesting approach to explore . As for the incorporated sorbent, multiwalled carbon nanotubes (MWCNTs) that can provide a large surface area, strong adsorption ability and also chemical stability are considered.…”

Section: Introductionmentioning

confidence: 99%

New sulfonate composite functionalized with multiwalled carbon nanotubes with cryogel solid-phase extraction sorbent for the determination of β-agonists in animal feeds

et al. 2015

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A new mixed-mode cation-exchange sulfonate composite functionalized with multiwalled carbon nanotubes with polyvinyl alcohol cryogel was fabricated and used for the first time as a solid-phase extraction sorbent for the determination of β-agonists in animal feeds. Feed samples were extracted with 0.20 M phosphoric acid and methanol (1:4, v/v) using ultrasonication, cleaned-up using the developed sorbent to which the β-agonists bound then finally eluted with 5.0% ammonia in methanol and analyzed by high-performance liquid chromatography. Various parameters that affected the extraction efficiency were optimized. Under the optimal conditions, the developed sorbent strongly interacted with β-agonists by cationic exchange and hydrophobic and hydrophilic interactions, that provided a high extraction efficiency in the range of 92.8 ± 3.7-104.4 ± 2.3% over a range of 0.04-2.0 mg/kg for salbutamol and ractopamine, and 0.40-8.0 mg/kg for clenbuterol. The relative standard deviations were less than 6.0%. The developed method was successfully applied for the determination of β-agonists in various types of animal feed and effectively reduced any matrix interference.

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Cryogels-versatile tools in bioseparation

Cited by 162 publications

References 125 publications

Immobilization of amyloglucosidase onto macroporous cryogels for continuous glucose production from starch

Immobilization of amyloglucosidase onto macroporous cryogels for continuous glucose production from starch

Removal of organic water pollutant using magnetite nanomaterials embedded with ionic copolymers of 2‐acrylamido‐2‐methylpropane sodium sulfonate cryogels

New sulfonate composite functionalized with multiwalled carbon nanotubes with cryogel solid-phase extraction sorbent for the determination of β-agonists in animal feeds

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