Cryopreservation and ensuing in vitro fertilization ability of boar spermatozoa from epididymides stored at 4°C

Kikuchi, Kazuhiro; Nagai, Takashi; Kashiwazaki, Naomi; Ikeda, H; Noguchi, Junko; Shimada, Arata; Soloy, E.; Kaneko, Hiroyuki

doi:10.1016/s0093-691x(98)00166-6

Cited by 180 publications

(150 citation statements)

References 16 publications

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“…This depends on the decomposition and dehydration process of the tissues of the vas deferens lumen and of the cauda epididymidis that occurs progressively after death, as also suggested by other authors in ibex, boar and mice (Kikuchi et al, 1998;Songsasen et al, 1998;Santiago-Moreno et al, 2009).…”

Section: Discussionmentioning

confidence: 54%

“…In rams, at least for total motility, this situation occurred at 48 h of testicle conservation at environment temperature (Kaabi et al, 2003). In refrigerated goat samples instead, a significant decrease in these parameters occurred later, at 48 h of testicle conservation, which was also observed in boars (Kikuchi et al, 1998). Therefore, the effect of 5°C refrigeration also has a positive impact on post-thaw samples, allowing a lower decline in buck epididymal sperm quality through the different PMTs.…”

Section: Discussionmentioning

confidence: 86%

See 1 more Smart Citation

Effect of testicle postmortem storage on goat frozen-thawed epididymal sperm quality as a tool to improve genebanking in local breeds

Turri

Madeddu

Gliozzi

et al. 2014

Animal

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The interest to develop assisted reproductive technologies and cryobanking for farm animal genetic resource conservation has recently increased. However, cryopreservation for ex-situ management of genetic diversity sometimes is not routinely feasible, owing to the lack of facilities (AI centres, laboratories) and expertise near the local breed farming area. In these cases, epididymal sperm obtained from slaughtered or castrated animals, associated with the possibility of managing rather long periods between animal death, sperm recovery and freezing, would increase the opportunities to create semen storages. This investigation addresses the pre-freeze/post-thaw quality of goat epididymal sperm as a function of testicle storage temperature (environment or +5°C) and time elapsed between animal's death and sperm recovery (0, 24, 48, 72 h) to establish the optimal protocols for the recovery and cryopreservation of epididymal sperm in this species. Testicles of 50 mature bucks collected at the abattoir were divided in two groups: half of the testicles (n = 50) were transported to the laboratory at environment temperature (E), whereas the remaining half (n = 50) at a refrigeration temperature (R) of +5°C. In the two groups (E) and (R), one testicle from each pair was processed after slaughter forming the time 0 groups (0E and 0R). The contralateral testicle was processed after 24, 48 or 72 h of storage, at the corresponding temperature. Sperm motility and kinetic parameters, viability and morphology were assessed in pre-freeze and post-thaw samples. Until 48 h postmortem, both E and R temperatures are able to maintain good pre-freeze epididymal sperm quality. After 48 h postmortem, R temperature is fundamental to reduce epididymal sperm quality decay in pre-freeze samples. Moreover, testicle refrigeration also has a positive impact on post-thaw samples, allowing a lower decline through time considering total motility, kinetics parameters, sperm viability and sperm abnormalities. Therefore, when sperm cryopreservation is not immediately practicable, goat testicles should be transported and stored at 5°C up to a maximum of 48 h postmortem to ensure an acceptable sperm quality.

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Section: Discussionmentioning

confidence: 54%

Section: Discussionmentioning

confidence: 86%

Effect of testicle postmortem storage on goat frozen-thawed epididymal sperm quality as a tool to improve genebanking in local breeds

Turri

Madeddu

Gliozzi

et al. 2014

Animal

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show abstract

“…About 20 oocytes were transferred into 100 µl Pig-FM (Suzuki et al, 2002) droplets covered by paraffin oil. They were coincubated then with 1 × 10 5 /ml frozenthawed epididymal spermatozoa (Kikuchi et al, 1998) for 3 h at 39°C under 5% CO 2 , 5% O 2 and 90%N 2 . After removal of spermatozoa attached to the surface of zona pellucida by gentle pipetting with a fine glass pipette, IVC was performed in IVC-PyrLac for 10 h.…”

Section: Ivf and Ivc Of Porcine Oocytesmentioning

confidence: 99%

Synchronization of In Vitro Maturation in Porcine Oocytes

Somfai

Hirao

2016

Methods in Molecular Biology

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“…The epididymides were refrigerated at 4 C, then spermatozoa were collected with Collecting Solution (Table 1) and frozen using Niwa and Sasaki Freezing extenders (NSF) ( Table 1) [8] as previously described [3]. Briefly, epididymides from 2 boars of Large White breed (Boar A and B) were collected at a local slaughterhouse.…”

Section: Preparation Of Spermatozoamentioning

confidence: 99%

“…Such a methodology may be useful when a male animal suddenly dies on a farm or in a field far from a laboratory. It has been reported that spermatozoa collected from epididymides stored at 4 C for a few days can maintain their motility (pig [3]; horse and shika deer [4]) and that they can be frozen and retain the ability to fertilize oocytes in vitro (pig [3]; monkey [5]). The ability of frozen-thawed epididymal spermatozoa to produce offspring after Fallopian insemination (mouse [1]) or in vitro fertilization (IVF) of oocytes matured in vitro (IVM) followed by embryo transfer (ET) (pig [6]; cow [7]) has also been reported.…”

mentioning

confidence: 99%

Reproduction in Pigs Using Frozen-Thawed Spermatozoa from Epididymis Stored at 4C.

Kikuchi

Kashiwazaki

Nagai

et al. 1999

Journal of Reproduction and Development

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Abstract. The ability of frozen-thawed boar spermatozoa obtained from epididymides stored at 4 C for 1 day to produce piglets after Fallopian insemination or in vitro fertilization (IVF) of in vitro matured (IVM) oocytes was examined. To test their in vivo fertilization ability, frozen-thawed spermatozoa from 2 boars, which had already shown IVF ability, were introduced into the Fallopian tubes of estrus-synchronized gilts. Embryo collection on the 7th day post-insemination revealed that one of two batches of spermatozoa had an ability of fertilization in vivo and that fertilized oocytes could develop to blastocysts. Three of 6 gilts inseminated with one batch of spermatozoa became pregnant and one of them farrowed 2 normal piglets (1 male and 1 female). IVM oocytes fertilized in vitro by the same spermatozoa were transferred to 3 recipients (200 oocytes per recipient). One of them became pregnant and farrowed 5 normal piglets (3 males and 2 females). The results indicate that boar spermatozoa collected from epididymides stored for 1 day at 4 C and then frozen have the ability to produce piglets. The new cryopreservation protocol and reproductive technology described here can enhance conservation of boar genetic resources when the collection site of the epididymides is far from a laboratory.

show abstract

Cryopreservation and ensuing in vitro fertilization ability of boar spermatozoa from epididymides stored at 4°C

Cited by 180 publications

References 16 publications

Effect of testicle postmortem storage on goat frozen-thawed epididymal sperm quality as a tool to improve genebanking in local breeds

Effect of testicle postmortem storage on goat frozen-thawed epididymal sperm quality as a tool to improve genebanking in local breeds

Synchronization of In Vitro Maturation in Porcine Oocytes

Reproduction in Pigs Using Frozen-Thawed Spermatozoa from Epididymis Stored at 4C.

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