Cryopreservation and In Vitro banking: a cool subject – Preface from the editors

Ochatt, Sergio; Lambardi, Maurizio; Panis, Bart; Pathirana, Ranjith; Revilla, M. A.; Wang, Qiao‐Chun

doi:10.1007/s11240-020-01985-1

Cited by 11 publications

(5 citation statements)

References 26 publications

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“…Gene banks are international collections that collect, conserve, and make available accessions of crops, crop wild relatives, tree species, and endangered plants using procedures and conditions that uphold the highest standards. ey play an important role in advancing plant breeding programs (Ochatt et al, 2021) and protecting endangered species (Pence, 2011). Traditional tools of in situ and ex situ conservation are supplemented by cryopreservation techniques applied for the long-term storage of plant germplasms.…”

Section: Introductionmentioning

confidence: 99%

Polish Cryobanks: Research and Conservation of Plant Genetic Resources

et al. 2022

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To date, the use of liquid nitrogen (LN) in plant gene banks is relatively limited. Globally, in 2021, approximately 10,000 accessions were cryopreserved, and their explants were derived from in vitro plants. In Europe, out of 500 banks, only 20 use cryogenic temperatures to store plant germplasms. The implementation of seven conservation projects in Poland starting in the 1990s meant that the gene banking system in this country began to gradually develop. Plant gene bank is mainly based on the collection of seeds and makes little use of plant tissues, including those from in vitro culture. From the point of view of systematics, plant material belonging to spermatophytes dominates in Polish gene banks, while spore plants are rarely represented. This review represents the first summary of gene cryobank activities and resources in Poland. It contains a brief overview of the ex situ plant protection programs aimed at the use of LN and presents the characteristics of four existing gene cryobanks in this country. Special attention is devoted to the presentation of studies on the cryopreservation of seeds, as well as of plant tissues, such as embryonic axes and plumules of trees, and fern gametophytes, that make a permanent contribution to gene bank resources.

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Section: Introductionmentioning

confidence: 99%

Polish Cryobanks: Research and Conservation of Plant Genetic Resources

et al. 2022

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“…Cryopreservation is a process that imposes significant stress on cells. Monitoring the activity of antioxidant enzymes during cryopreservation may provide a better comprehension of plant metabolism and response to the production of ROSs [ 21 , 22 ]. The analysis of the redox state realized in the present study revealed that the incubation of G. chacoensis ECs in the cryoprotectant solution resulted in a considerable increase in MDA content, independent of the time of incubation used—60 or 120 min—in addition to a high H 2 O 2 content and CAT activity.…”

Section: Discussionmentioning

confidence: 99%

“…The generation of reactive oxygen species (ROSs), such as hydrogen peroxide (H 2 O 2 ), superoxide radical (O 2 •− ), hydroxyl radical (OH • ), and singlet oxygen ( 1 O 2 ) [ 20 ], is inevitable during the multiple steps of cryopreservation [ 21 , 22 ]. An excessive imbalance between ROS production and the activity of antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and guaiacol peroxidase (GPX), could result in oxidative stress, which disrupts cellular homeostasis and can lead to membrane damage by lipid peroxidation, protein oxidation, DNA damage, and, in the most serious case, results in programmed cell death [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Enzymatic Antioxidant System Activation Assures the Viability of Guadua chacoensis (Bambusoideae, Poaceae) Embryogenic Cultures during Cryopreservation

Polesi

Goeten

Fraga

et al. 2023

Plants

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This study aimed to establish a cryopreservation protocol for G. chacoensis embryogenic cultures (ECs) and to investigate the role of antioxidant enzymes activities during cryopreservation. The growth dynamics of cell suspensions were also investigated, followed by a phytotoxicity test to assess the ECs’ ability to tolerate the use of cryoprotective solutions for different incubation times (0, 30, 60, 120, and 240 min). We evaluated the EC redox state in three steps of cryopreservation: after incubation in cryoprotection solution, after thawing, and 60 days after regrowth. Our results showed that the ECs support the use of cryoprotective solution until 120 min, showing phytotoxic effects with 240 min of incubation. This study reports a 100% survival of the cultures and a 10% increase ratio in fresh material for both incubation times tested (60 and 120 min). Increased malonaldehyde content was identified after incubation in the cryoprotective solution. An increase in the activities of catalase and ascorbate peroxidase was also identified in the subsequent steps, suggesting that the activation of antioxidant enzymes is essential for maintaining cell homeostasis during cryopreservation.

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“…Conservation of plant genetic resources is an important challenging issue. As these resources are lost and genetically eroded due to biotic and abiotic stresses or human errors, nevertheless cryopreservation is regarded as a reliable method of conserving plant resources wherethrough samples are stored in LN (-196°C), which allows plant materials to be safely stored for long-lasting time (Shibli et al 2006;Ochatt et al 2021). At this temperature, all cellular activities such as cell division, metabolism, and biochemical activities are stopped, and samples can be stored for a long time period without modi cation (Walters et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Pretreatment with abscisic acid accompanied by sucrose improves callus survival after cryopreservation of hazel (Corylus avellana L.) by desiccation

Nanas

Moieni

Mokhtassi‐Bidgoli

2022

Plant Cell Tiss Organ Cult

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A simple cryopreservation method (desiccation) is examined on Corylus avellana L. callus in current study. The effect of abscisic acid (ABA) concentration, preculture length on Murashige and Skoog (MS) medium containing ABA + 10% sucrose, and storage length in liquid nitrogen (LN) on callus survival rate were investigated. After cryo-storage, the calli were heated at 40°C followed by cold tap water for one minute, then transferred to the recovery medium. Calli survival was assessed 6-8 weeks after exiting from LN. There was a signi cant difference between ABA concentrations and different culture periods on medium containing mentioned hormone in survival rate but no signi cant difference was observed between the storage periods in LN. In the present study, the highest survival rate (45/79%) was obtained in both the treatment of 20 days preculture on medium containing 2 mg l − 1 ABA following 120 minutes desiccation-one day storage in LN and 150 minutes desiccation-one month storage in LN. Key MessageDesiccation method with the help of ABA and sucrose was an effective method in the successful cryopreservation of Corylus avellana L. callus.

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Cryopreservation and In Vitro banking: a cool subject – Preface from the editors

Cited by 11 publications

References 26 publications

Polish Cryobanks: Research and Conservation of Plant Genetic Resources

Polish Cryobanks: Research and Conservation of Plant Genetic Resources

Enzymatic Antioxidant System Activation Assures the Viability of Guadua chacoensis (Bambusoideae, Poaceae) Embryogenic Cultures during Cryopreservation

Pretreatment with abscisic acid accompanied by sucrose improves callus survival after cryopreservation of hazel (Corylus avellana L.) by desiccation

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