Cryopreservation of Peach Shoot Tips by Encapsulation Dehydration

Damiano, C.; Sgueglia, A.; Arias, M.; Frattarelli, A.; Condello, E.; Caboni, E.

doi:10.17660/actahortic.2011.918.13

Cited by 3 publications

(1 citation statement)

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“…It should be noted that for long-term storage of the peach gene pool, the method of cryopreservation of dormant buds is not inferior in efficiency to the methods of encapsulationdehydration [45] and vitrification of the peach shoot tips [46]. In the first case, the viability of the peach shoot tips after cryopreservation was 33-36%; in the second case, it was 60%, which is comparable with our results-43.3-55.6% of viable buds after cryopreservation.…”

Section: Resultsmentioning

confidence: 99%

Post-Cryogenic Viability of Peach (Persica vulgaris Mill.) Dormant Buds from the VIR Genetic Collection

et al. 2022

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The long-term storage of the genetic resources of fruit crops for breeding needs can be freely developed by cryopreservation cuttings with dormant buds in liquid nitrogen vapor, but so far, this method has not been practically used for peach. Cuttings with dormant buds of five peach varieties growing in the field gene bank at Krymsk Experiment and Breeding Station of VIR were collected for cryopreservation in 2019–2021. The three-factor analysis of variance showed that the viability of peach cuttings was significantly affected by the year (p < 0.001) and variety (p < 0.001). According to the three-year average characteristics of the cultivars, the analysis of variance showed a significant difference in the viability of the cultivars after cryopreservation (p = 0.004). According to the results of the three years of study, cvs. ‘Podarok Kryma’ (43.3%) and ‘Lucky 24 B’ (44.4%) showed the lowest viability after cryopreservation, significantly lower than cvs. ‘Baby Gold’ (54.4%) and ‘Ustojchivy 90′ (55.6%). Cv. ‘Lyubimets Krasnodara’ (48.9%) occupied an intermediate position. These viability values exceeded the minimum requirement for samples subjected to long-term cryogenic storage in a cryobank. Low-temperature storage of peach cuttings at –5 °C can be used for short-term preservation. After low-temperature storage, the viability of peach cutting amounted to an average of 67.1%.

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Section: Resultsmentioning

confidence: 99%

Post-Cryogenic Viability of Peach (Persica vulgaris Mill.) Dormant Buds from the VIR Genetic Collection

et al. 2022

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Cryopreservation of Plant Cells

Keller¹,

Senula²,

Höfer³

et al. 2013

Encyclopedia of Industrial Biotechnology

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Cryopreservation, storage of living tissues in or above liquid nitrogen, is increasingly used to preserve germplasm in the long term. When cooling plant tissue to low temperatures, water may destroy cells by osmotic drying or disruption of organelles by forming ice crystals. The organisms accumulate cryoprotective substances to adapt to freezing. Cryoprotectants trigger glass transition (vitrification), when temperature is cooled down rapidly. In the course of cryopreservation, tissues are imposed by various influences causing oxidative stress: wounding, dehydration, and direct liquid nitrogen effect. Occurring reactive oxygen species may be measured and antioxidant substances may be added. Cell structure alterations, water status, regeneration from cryopreservation injury, and genetic and epigenetic changes, which may occur, were investigated. Various plant tissues were cryopreserved: dedifferentiated cells and tissues; shoot tips; axillary buds and other meristematic plant parts; dormant buds of woods species, seeds, embryos, and embryo axes; and pollen. Several main methods were used: slow freezing, vitrification, and encapsulation. Liquid nitrogen can also be used to eliminate viruses from the organs (cryotherapy). The existence of endogenous or contaminating microorganisms needs special attention. Furthermore, technological considerations and the use of cryopreservation to maintain transgenic or patented material need to be mentioned.

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