Cryopreservation of Pig Semen Using a Quercetin-Supplemented Freezing Extender

Bang, Seonggyu; Tanga, Bereket Molla; Fang, Xun; Seong, Gyeonghwan; Saadeldin, Islam M.; Qamar, Ahmad Yar; Lee, Sanghoon; Kim, Keun-Jung; Park, Yunjae; Nabeel, Abdelbagi Hamad Talha; Yu, Il-Jeoung; Cooray, Akila; Lee, Kyu Pil; Cho, Jongki

doi:10.3390/life12081155

Cited by 8 publications

(5 citation statements)

References 65 publications

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“…In the last decade, a great deal of attention has been focused on the favorable effects of antioxidants on the quality of cryopreserved boar sperm and fertilizing capacity, such as vitamin E [ 5 , 6 ], glutathione [ 7 , 8 , 9 ], l -cysteine [ 10 , 11 ], N -acetyl l -cysteine [ 3 , 12 ] and gamma-oryzanol [ 13 , 14 ]. In recent years, several plant-derived polyphenols such as quercetin (flavonoids) [ 15 ], resveratrol (non-flavonoids) [ 16 ], and curcumin [ 17 ] also revealed their potential antioxidant activities for boar sperm.…”

Section: Introductionmentioning

confidence: 99%

The Beneficial Effect of Resveratrol on the Quality of Frozen-Thawed Boar Sperm

Kaeoket,

Chanapiwat

2023

Animals

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This study aimed to determine the effect of resveratrol and its optimal concentration on the quality of frozen-thawed (FT) boar sperm. Semen ejaculates were obtained from 13 Duroc boars aged between 1.5 and 3 years. The sperm sample was separated into 7 groups based on the concentrations of resveratrol in the freezing extender, which were 0 (control), 25, 50, 75, 100, 125, and 250 µM, respectively. The sperm was frozen using liquid nitrogen vapor and thawed at 50 °C for 12 s. After thawing, total motility, progressive motility, viability, intact acrosomes, mitochondrial membrane potential and level of MDA were assessed. The supplementation of 50–100 µM resveratrol improved the sperm motility and viability of FT sperm in comparison to the control group (p < 0.05). Furthermore, the 50 µM resveratrol group was significantly more protective than the control group in terms of intact acrosome, mitochondrial membrane potential, and level of MDA (p < 0.05). Nonetheless, the detrimental effect of resveratrol was found at a concentration of 250 µM. In conclusion, the addition of 50–100 µM resveratrol to a freezing extender is the optimal concentration for enhancing the quality of cryopreserved boar sperm.

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Section: Introductionmentioning

confidence: 99%

The Beneficial Effect of Resveratrol on the Quality of Frozen-Thawed Boar Sperm

Kaeoket,

Chanapiwat

2023

Animals

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“…Yeste et al demonstrated the significance of Slo1 in the capacitation and acrosome reactions of boar spermatozoa [24,25]. One of our previous reports showed that quercetin can inhibit human Slo3 current [19], and later, we found quercetin also depolarizes cryopreserved boar sperm [39]. This suggested that Slo3 in boar sperm may be functionally significant in maintaining its membrane potential.…”

Section: Discussionmentioning

confidence: 51%

Pharmacological Evidence Suggests That Slo3 Channel Is the Principal K+ Channel in Boar Spermatozoa

Cooray

Kim

Nirujan

et al. 2023

IJMS

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Sperm ion channels are associated with the quality and type of flagellar movement, and their differential regulation is crucial for sperm function during specific phases. The principal potassium ion channel is responsible for the majority of K+ ion flux, resulting in membrane hyperpolarization, and is essential for sperm capacitation-related signaling pathways. The molecular identity of the principal K+ channel varies greatly between different species, and there is a lack of information about boar K+ channels. We aimed to determine the channel identity of boar sperm contributing to the primary K+ current using pharmacological dissection. A series of Slo1 and Slo3 channel modulators were used for treatment. Sperm motility and related kinematic parameters were monitored using a computer-assisted sperm analysis system under non-capacitated conditions. Time-lapse flow cytometry with fluorochromes was used to measure changes in different intracellular ionic concentrations, and conventional flow cytometry was used to determine the acrosome reaction. Membrane depolarization, reduction in acrosome reaction, and motility parameters were observed upon the inhibition of the Slo3 channel, suggesting that the Slo3 gene encodes the main K+ channel in boar spermatozoa. The Slo3 channel was localized on the sperm flagellum, and the inhibition of Slo3 did not reduce sperm viability. These results may aid potential animal-model-based extrapolations and help to ameliorate motility and related parameters, leading to improved assisted reproductive methods in industrial livestock production.

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“…To assess gene expression, the total RNA for the control and myo-inositol-treated groups was prepared using the TRI method [ 34 ] and TRIZOL reagent ® (Molecular Research Center, Inc., Cincinnati, OH, USA) in accordance with the manufacturer’s instructions. Briefly, after thawing at 37 °C for 25 s, spermatozoa were washed twice with DPBS (centrifugation at 160× g for 2 min), and the supernatant was removed.…”

Section: Methodsmentioning

confidence: 99%

Antioxidant Effects of Myo-Inositol Improve the Function and Fertility of Cryopreserved Boar Semen

Osman,

Lee,

Almubarak

et al. 2023

Antioxidants

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During cryopreservation, sperm undergoes structural and molecular changes such as ice crystal formation, DNA fragmentation, and reactive oxygen species (ROS) production, leading to decreased sperm quality after thawing. Antioxidants play a crucial role in preventing these damages, both in vivo and in vitro. One potent antioxidant is myo-inositol, known for its protective effects on sperm against ROS. This study aimed to investigate the protective effect of myo-inositol on cryopreserved boar semen. The semen was diluted, cooled, and cryopreserved using a BF5 extender. It was then divided into five groups: control and different concentrations of myo-inositol (0.5, 1, 1.5, and 2 mg/mL). The post-thaw evaluation included assessments of motility, viability, acrosome integrity, mitochondrial membrane potential (MMP), caspase activity, gene expression, ROS levels, apoptosis, and IVF with treated semen. Results showed that myo-inositol at 0.5 mg/mL improved motility, acrosome integrity, and fertilization ability. It also reduced the expression of pro-apoptotic genes and increased SMCP expression. Lower concentrations also demonstrated improved viability and reduced apoptosis and ROS levels. In conclusion, myo-inositol treatment during cryopreservation improved sperm quality, reduced apoptosis and ROS levels, and enhanced fertility rates in boar semen.

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Cryopreservation of Pig Semen Using a Quercetin-Supplemented Freezing Extender

Cited by 8 publications

References 65 publications

The Beneficial Effect of Resveratrol on the Quality of Frozen-Thawed Boar Sperm

The Beneficial Effect of Resveratrol on the Quality of Frozen-Thawed Boar Sperm

Pharmacological Evidence Suggests That Slo3 Channel Is the Principal K+ Channel in Boar Spermatozoa

Antioxidant Effects of Myo-Inositol Improve the Function and Fertility of Cryopreserved Boar Semen

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