Cryopreservation of Testicular Tissue

Honaramooz, Ali

doi:10.5772/32338

Cited by 9 publications

(13 citation statements)

References 98 publications

(136 reference statements)

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“…Peroxidative damage is an important factor in sperm function impairment. As is well known, CPAs have different ways of protecting cells from freezing injuries (Honaramooz, 2012). Scavenging of oxygen free radicals and preventing oxidative stress to the cells (Fleck et al, 2000) seem to be important pathways.…”

Section: Discussionmentioning

confidence: 99%

“…Testicular tissue cryopreservation can avoid mechanical damage in the process of cell digestion and reduce the phenomenon of cell hypoxia. This makes the preservation of animal species more efficient (Honaramooz, 2012). By cryopreservation of the collected testis tissues, male fertility could be preserved in the long term and used for further assisted reproduction (Kishikawa et al, 1999;Martinez et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

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Effects of different cryoprotectants on the cryopreservation of cattle testicular tissue

et al. 2015

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Abstract. Cryopreservation of testicular tissue is a new option in fertility preservation for prepubertal male animals. The purpose of this study was to explore the effects of different cryoprotectant agents (CPAs) at various concentrations on testes after the cryopreservation of calf testicular tissue. These experiments selected dimethyl sulfoxide (DMSO), glycerol, propylene glycol (PrOH), and sucrose as CPAs in varying doses (2.5, 5, 7.5, 10, 12.5, 15, 17.5, and 20 %; v/v) in 8-month-old calf testicular tissue that was frozen and preserved. Then, cell viability, testosterone production, malondialdehyde (MDA) level, and superoxide dismutase (SOD) level were detected and analyzed following cryopreservation. The results showed that the optimal concentrations of DMSO, PrOH, glycerol, and sucrose were 10, 10, 7.5, and 10 %, respectively. Compared to the optimal concentrations of CPAs, cell viability and testosterone production decreased significantly at a lower and higher CPA concentration (P <0.05). At the optimal concentrations of CPAs, the DMSO group showed higher cell viability and testosterone production than other CPA groups (P <0.05). Compared to the optimal concentration of CPAs, the MDA level increased and the SOD level decreased at a lower or higher concentration of CPAs, but there was no significant difference (P >0.05). Cell viability was significantly positively correlated with testosterone production (P <0.05). In conclusion, DMSO provided the most effective protection for calf testicular tissue cryopreservation and the optimal concentration was 10 %.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of different cryoprotectants on the cryopreservation of cattle testicular tissue

et al. 2015

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“…However, crossspecies transplantation of isolated testis cells from non-rodent species into the seminiferous tubules of immunodeficient mice did not result in complete spermatogenesis, likely as a result of the incompatibility of donor germ cells and recipient's supporting somatic cells (Dobrinski et al 1999, 2000, Nagano et al 2001, 2002. Testis tissue xenografting, on the other hand, can be used to study different aspects of testis function of a variety of donor species, including laboratory and domestic/non-domestic animals and even primates (Honaramooz et al 2002a, 2004, Schlatt et al 2002, Shinohara et al 2002, Oatley et al 2004, Snedaker et al 2004, Rathi et al 2005, Arregui et al 2008a, 2008b, 2013, Abrishami et al 2010a, Abbasi & Honaramooz 2011a, 2012, Gourdon & Travis 2011, Campos-Junior et al 2014. The key factor in the success of TTX is maintaining the structural integrity of the testis tissue, and allowing the somatic and germ cells to maintain normal interactions (Honaramooz et al 2002a).…”

Section: Rationale and Potential Applicationsmentioning

confidence: 99%

Potential and challenges of testis tissue xenografting from diverse ruminant species

Honaramooz¹

2019

Proc

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In 2002, we reported that small fragments of testis tissue from immature mouse, pig or goat donors grafted in recipient mice undergo development, maturation and complete spermatogenesis, including the generation of fertilisation-competent murine, porcine or caprine sperm. Testis tissue xenografting (TTX) was then successfully applied using a range of donor species including laboratory/domestic/non-domestic animals and primates. This system offers a novel in vivo model for the study of testis function, and a previously unavailable opportunity to produce sperm in the grafts from immature donors of diverse species. The TTX model also provides easier access for experimental manipulation of the grafted testis tissue or its environment in the recipient mouse; something that is not feasible in many donor species. This application will allow analysis of, for instance, the effects of new hormone regimens, drugs or toxicants on testis function, without experimentation in the target species. Grafting of fresh or preserved testis tissue also can be used as an invaluable tool for the conservation of fertility from immature individuals of valuable or endangered animals. Reviewed here are an overview of the contributions by the author and colleagues and a critical examination of the salient literature on TTX especially using ruminant donors, as well as examples of its variety of current and potential applications for research in male reproductive biology and technologies using ruminant models. The challenges facing optimisation of TTX model as well as its field/experimental uses, along with insights and suggested remedies, are also discussed.

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“…The cryopreserved immature testicular tissue can be later used for various assisted reproductive technologies (ART) [19]. The developmental stage of the testis determines the success of cryopreservation as immature testis differs from adult testis in its tissue texture and future developmental potential [20]. In adult males, cryopreserved testicular tissues are a source for sperm.…”

Section: Implications Of Gonadal Tissue Cryopreservationmentioning

confidence: 99%

Fertility preservation through gonadal cryopreservation

Devi

Goel

2016

Reprod Medicine & Biology

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Fertility preservation is an area of immense interest in today's society. The most effective and established means of fertility preservation is cryopreservation of gametes (sperm and oocytes) and embryos. Gonadal cryopreservation is yet another means for fertility preservation, especially if the gonadal function is threatened by premature menopause, gonadotoxic cancer treatment, surgical castration, or diseases. It can also aid in the preservation of germplasm of animals that die before attaining sexual maturity. This is especially of significance for valuable, rare, and endangered animals whose population is affected by high neonatal/juvenile mortality because of diseases, poor management practices, or inbreeding depression. Establishing genome resource banks to conserve the genetic status of wild animals will provide a critical interface between ex-situ and in-situ conservation strategies. Cryopreservation of gonads effectively lengthens the genetic lifespan of individuals in a breeding program even after their death and contributes towards germplasm conservation of prized animals. Although the studies on domestic animals are quite promising, there are limitations for developing cryopreservation strategies in wild animals. In this review, we discuss different options for gonadal tissue cryopreservation with respect to humans and to laboratory, domestic, and wild animals. This review also covers recent developments in gonadal tissue cryopreservation and transplantation, providing a systematic view and the advances in the field with the possibility for its application in fertility preservation and for the conservation of germplasm in domestic and wild species.

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Cryopreservation of Testicular Tissue

Cited by 9 publications

References 98 publications

Effects of different cryoprotectants on the cryopreservation of cattle testicular tissue

Effects of different cryoprotectants on the cryopreservation of cattle testicular tissue

Potential and challenges of testis tissue xenografting from diverse ruminant species

Fertility preservation through gonadal cryopreservation

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