Cryptic <i>Aspergillus</i> from clinical samples in the <scp>USA</scp> and description of a new species in section <i>Flavipedes</i>

Siqueira, João Paulo Zen; Wiederhold, Nathan P.; Gené, Josepa; García, Dania; Almeida, Margarete Teresa Gottardo de; Guarro, Josep

doi:10.1111/myc.12818

Cited by 16 publications

(8 citation statements)

References 66 publications

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“…6 ) and one of these sequences was therefore most likely incorrectly deposited. Apart from mentioned cases, A. micronesiensis was also reported by Siqueira et al. (2018) from canine urine sample.…”

Section: Discussionmentioning

confidence: 85%

“…(2014) , Hubka et al (2015) , Arzanlou et al. (2016) , Siqueira et al. (2018) , Crognale et al (2019) .…”

Section: Resultsmentioning

confidence: 99%

“…Another four species were described since then, namely, A. urmiensis described from hypersaline soils in Iran ( Arzanlou et al. 2016 ), A. suttoniae from human sputum in the USA ( Siqueira et al. 2018 ), A. olivimuriae from olive brine in Italy ( Crognale et al.…”

Section: Introductionmentioning

confidence: 99%

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Re-examination of species limits in Aspergillus section Flavipedes using advanced species delimitation methods and description of four new species

Sklenář¹,

Jurjević

Houbraken

et al. 2021

Studies in Mycology

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Since the last revision in 2015, the taxonomy of section Flavipedes evolved rapidly along with the availability of new species delimitation techniques. This study aims to re-evaluate the species boundaries of section Flavipedes members using modern delimitation methods applied to an extended set of strains (n = 90) collected from various environments. The analysis used DNA sequences of three house-keeping genes ( benA , CaM , RPB2 ) and consisted of two steps: application of several single-locus (GMYC, bGMYC, PTP, bPTP) and multi-locus (STACEY) species delimitation methods to sort the isolates into putative species, which were subsequently validated using DELINEATE software that was applied for the first time in fungal taxonomy. As a result, four new species are introduced, i.e. A. alboluteus , A. alboviridis , A. inusitatus and A. lanuginosus , and A. capensis is synonymized with A. iizukae . Phenotypic analyses were performed for the new species and their relatives, and the results showed that the growth parameters at different temperatures and colonies characteristics were useful for differentiation of these taxa. The revised section harbors 18 species, most of them are known from soil. However, the most common species from the section are ecologically diverse, occurring in the indoor environment (six species), clinical samples (five species), food and feed (four species), droppings (four species) and other less common substrates/environments. Due to the occurrence of section Flavipedes species in the clinical material/hospital environment, we also evaluated the susceptibility of 67 strains to six antifungals (amphotericin B, itraconazole, posaconazole, voriconazole, isavuconazole, terbinafine) using the reference EUCAST method. These results showed some potentially clinically relevant differences in susceptibility between species. For example, MICs higher than those observed for A. fumigatus wild-type were found for both triazoles and amphotericin B for A. ardalensis, A. iizukae, and A. spelaeus whereas A. lanuginosus, A. luppiae, A. movilensis, A. neoflavipes, A. olivimuriae and A. suttoniae were comparable to or more susceptible as A. fumigatus . Finally, terbinafine was in vitro active against all species except A. alboviridis .

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Section: Discussionmentioning

confidence: 85%

“…(2014) , Hubka et al (2015) , Arzanlou et al. (2016) , Siqueira et al. (2018) , Crognale et al (2019) .…”

Section: Resultsmentioning

confidence: 99%

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Re-examination of species limits in Aspergillus section Flavipedes using advanced species delimitation methods and description of four new species

Sklenář¹,

Jurjević

Houbraken

et al. 2021

Studies in Mycology

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“…Guinea et al and A. suttoniae J.P.Z. Siqueira et al were isolated from the sputum of humans [ 35 , 42 ], and A. alabamensis Balajee et al from the wounds of humans [ 43 ]. Whether others of these sections are potentially pathogenic requires future investigation.…”

Section: Discussionmentioning

confidence: 99%

New Species of Aspergillus (Aspergillaceae) from Tropical Islands of China

Wang

Zhuang

2022

JoF

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Aspergillus species are cosmopolitan and ubiquitous, closely related to human daily life. They are also of food, industrial and medical importance. From the examination of cultures isolated from soil samples collected on tropical islands of China, four new species of the genus were discovered based on phylogenetic analyses and morphological comparisons. Aspergillus xishaensis sp. nov. and A. neoterreus sp. nov. belong to sections Flavipedes and Terrei of subgenus Circumdati, and A. hainanicus sp. nov. and A. qilianyuensis sp. nov. are in sections Cavernicolarum and Nidulantes of subgenus Nidulantes. To accommodate A. hainanicus, a new series Hainanici was proposed. Detailed descriptions and illustrations of the new taxa were provided.

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“…The monophyletic genus Aspergillus currently contains 339 species [3]. The minority of these species were reported from human infections and are associated to the sections Fumigati [4], Flavi [5], Nidulantes [6], Usti [7], Cremei [8], Nigri [9] and Terrei [9,10] [9]. Even though the species within the section Terrei are taxonomically accepted species, they are referred to as cryptic species.…”

Section: Introductionmentioning

confidence: 99%

Cryptic species of Aspergillus section Terrei display essential physiological features to cause infection and are similar in their virulence potential in Galleria mellonella

et al. 2019

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Aspergillus species account for the majority of invasive mold infections in immunocompromised patients. Most commonly, members of the Aspergillus section Fumigati are isolated from clinical material, followed by isolates belonging to section Terrei. The section Terrei contains 16 accepted species. Six species were found to be of clinical relevance and studied for differences in growth adaptability and virulence potential. Therefore, a set of 73 isolates (22 A. terreus s.s., 8 A. alabamensis, 27 A. citrinoterreus, 2 A. floccosus, 13 A. hortai, and 1 A. neoafricanus) was studied to determine differences in (a) germination kinetics, (b) temperature tolerance, (c) oxygen stress tolerance (1% O 2 ), and (d) a combination of the latter two. Virulence potential of phialidic (PC) and accessory conidia (AC) was studied in G. mellonella larvae, using survival as read out. Further, the formation of AC was evaluated in larval tissue. All isolates were able to grow at elevated temperature and hypoxia, with highest growth and germination rates at 37°C. A. terreus s.s., A. citrinoterreus, and A. hortai exhibited highest growth rates. Virulence potential in larvae was inoculum and temperature dependent. All species except A. floccosus formed AC and germination kinetics of AC was variable. Significantly higher virulence potential of AC was found for one A. hortai isolate. AC could be detected in larval tissue 96 h post infection. Based on these findings, cryptic species of section Terrei are well adapted to the host environment and have similar potential to cause infections. ARTICLE HISTORY

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Cryptic Aspergillus from clinical samples in the USA and description of a new species in section Flavipedes

Abstract: Correct identification of cryptic species is crucial to reveal new potential pathogens, to gather accurate epidemiological data and to choose an appropriate treatment.

Cited by 16 publications

References 66 publications

Re-examination of species limits in Aspergillus section Flavipedes using advanced species delimitation methods and description of four new species

Re-examination of species limits in Aspergillus section Flavipedes using advanced species delimitation methods and description of four new species

New Species of Aspergillus (Aspergillaceae) from Tropical Islands of China

Cryptic species of Aspergillus section Terrei display essential physiological features to cause infection and are similar in their virulence potential in Galleria mellonella

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