Cryptococcus neoformans meningitis in a heart transplanted patient difficult diagnosis: a case report

Abstract

Study purposed to investigate the role of several infective agents in multiple sclerosis. Patients and methods: Fifty nine patients with MS have been investigated. Among them the first group-19 patients with primary progressive MS, the second group-24 patients with relapse remitting MS and the third group-16 patients with secondary progressive MS. Several infections (Chlamydia pneumoniae, mycoplasma hominis, herpes virus 6) were detected in 4ml CSF. DNA was isolated from CSF and PCR method according to kit instruction (Amplification N15 kb, temperature over 65°C-Maximbio, USA). The length of the DNA was detected by agarose gel electrophoresis. CSF oligoclonal bands were detected by agarose gel electrophoresis with Coomassie Blue staining.Statistics performed by SPSS-11.0. Results: Chlamydia pneumoniae found to be positive in CSF of 11 (45%) patients with relapse remitting MS and in 7 (43%) patients with secondary progressive MS. Mycoplasma hominis was positive in CSF of 2 (8%) patients with relapse remitting MS. Herpes virus 6 was positive in 1(5%) patients with primary progressive MS and in 1(5%) patient with secondary progressive MS. Positive correlation was found between presence of CSF Chlamydia pneumonie and CSF count of oligoclonal bands (r = +0.27, pb0.01). Multiple logistic regression analysis revealed the significance of infective agents for mean predicted probability of development of relapse remitting MS. Conclusion: Probably, infective agents play the important role in immunology and clinical course of MS.