Cryptosporidium spp. CP15 and CSL protein-derived synthetic peptides’ immunogenicity and in vitro seroneutralisation capability

Avendaño, Catalina; Jenkins, Mark C.; Méndez-Callejas, Gina; Oviedo, Jairo; Guzmán, Fanny; Patarroyo, Manuel A.; Sánchez-Acedo, Caridad; Quílez, Joaquín

doi:10.1016/j.vaccine.2018.09.044

Cited by 7 publications

(11 citation statements)

References 53 publications

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“…So far, over 30 Cryptosporidium antigens have been identified in the early interactions between the pathogens and host cells, either as surface proteins or as part of the apical complex [8]. CP15, which is located on sporozoite surface, could stimulate antibody production capable of neutralizing parasite entry to in vitro culture cells [34]. The monoclonal antibodies directed to circumsporozoite-like (CSL) protein were shown to completely neutralize sporozoite infectivity in vitro and limit in vivo infection in a mouse model [35].…”

Section: Discussionmentioning

confidence: 99%

Cryptosporidium parvum gp40/15 Is Associated with the Parasitophorous Vacuole Membrane and Is a Potential Vaccine Target

Cui

Wang

et al. 2020

Microorganisms

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Cryptosporidium parvum is a zoonotic intracellular protozoan responsible for the diarrheal illness cryptosporidiosis in humans and animals. Although a number of zoite surface proteins are known to be expressed during, and believed to be involved in, attachment and invasion of host cells, the molecular mechanisms by which C. parvum invades the host epithelial cells are not well understood. In the present study, we investigated the gene expression patterns, protein localization in developmental stages in culture, and in vitro neutralization characteristics of Cpgp40/15 and Cpgp40. Indirect immunofluorescence assay showed that Cpgp40/15 is associated with the parasitophorous vacuole membrane (PVM) during intracellular development. Both anti-gp40/15 and anti-gp40 antibodies demonstrated the ability to neutralize C. parvum infection in vitro. Further studies are needed to fully understand the specific role and functional mechanism of Cpgp40/15 (or gp40/15 complex) in the invasion of the host or in the PVM and to determine the feasibility of gp40/15 as a vaccine candidate for cryptosporidiosis in vivo.

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Section: Discussionmentioning

confidence: 99%

Cryptosporidium parvum gp40/15 Is Associated with the Parasitophorous Vacuole Membrane and Is a Potential Vaccine Target

Cui

Wang

et al. 2020

Microorganisms

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“…Parasites of the genus Cryptosporidium spp. they are opportunistic and have zoonotic potential and can cause gastroenteric infections, via fecal-oral route or by the ingestion of contaminated water and food (Avendaño et al, 2018;Mcnair et al, 2018;Hemphill et al, 2019), with their sporulated oocysts (Lippuner et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Limited access to disinfectant agents, the absence of effective drugs and vaccines associated with the ability of oocysts to develop infection, makes cryptosporidiosis very important in public health (Askari et al, 2016). In developing countries, it is considered a threat, as it causes malnutrition, death of babies and immunocompromised individuals (Ryan et al, 2016;Avendaño et al, 2018;Mcnair et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…For now, 40 species of Cryptosporidium have been described, among which 19 infect humans (Robinson;Elwin & Chalmers, 2019) This agent causes severe diarrhea in patients considered to be at high risk (Askari et al, 2016;Avendaño et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…In immunocompromised patients, who may not respond to immunization and consequently develop the chronic form of the disease, it is still a challenge to develop a vaccine. However, there is an alternative to immune stimulation of B cells (Avendaño et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Vaccine development for cryptosporidiosis: Systematic review

Silva

Oliveira

Marta

et al. 2021

RSD

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Cryptosporidium spp. are opportunistic parasites with zoonotic potential transmitted by ingestion of contaminated water and food, the infection consists of severe diarrhea leading to the death of babies and immunocompromised individuals. In the veterinary, it can generate economic losses due to the death of calves, these animals being a possible source of transmission of the parasite. Nitazoxanide and halofuginone are the only drugs approved for treatment in humans and calves respectively, however there are restrictions on their use. There is still no vaccine against cryptosporidiosis in humans or animals and its development is a great challenge. Our objective with the present work was to write a systematic review of the literature addressing the progress of studies on vaccines against cryptosporidiosis. The recommended guidelines for RSL were used, with the aid of the StArt software (State of the Art through Systematic Rewiews). The research took place in the databases: Lilacs, PubMed, Scielo, Science Direct, Scopus, Embase and Medline. In 30% of the selected articles, the calves were the study animal and in 50% the mice; 40% of the studies were with vaccines derived from recombinant proteins and 30% of the research was aimed at inhibiting the parasite from entering cells and activating the host's protective immunity. So far, a vaccine with no proven efficacy or an ideal cost-benefit has been developed. Many candidate antigenic targets for a vaccine have been characterized, in addition to elucidating the immunogenicity mechanism of Cryptosporidium spp. in the infected individual. However, another antigenic targets for a vaccine can be searched in future studies.

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Advances in therapeutic and vaccine targets for Cryptosporidium: Challenges and possible mitigation strategies

Rahman

Zhou

et al. 2022

Acta Tropica

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Cryptosporidium spp. CP15 and CSL protein-derived synthetic peptides’ immunogenicity and in vitro seroneutralisation capability

Cited by 7 publications

References 53 publications

Cryptosporidium parvum gp40/15 Is Associated with the Parasitophorous Vacuole Membrane and Is a Potential Vaccine Target

Cryptosporidium parvum gp40/15 Is Associated with the Parasitophorous Vacuole Membrane and Is a Potential Vaccine Target

Vaccine development for cryptosporidiosis: Systematic review

Advances in therapeutic and vaccine targets for Cryptosporidium: Challenges and possible mitigation strategies

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