2003
DOI: 10.1016/s0006-291x(03)00379-6
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Crystallization and preliminary X-ray structural studies of hemoglobin A2 and hemoglobin E, isolated from the blood samples of β-thalassemic patients

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Cited by 15 publications
(8 citation statements)
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“…It is speculated that δ116Arg could make an extra hydrogen bond at the α 1 δ 1 interface and δ126Met (H4) could make intra-subunit non-polar contacts with δ11Val of helix A ( 45 ). However, a recent X-ray crystal studies of Hb A 2 did not support these additional interactions, but non-polar contacts formed between helices A and H were found, which presumably contribute to the higher thermal stability of Hb A 2 ( 10, 46 ). Notably, rHb WM and rHb AE, also possess Arg at position 116 of their β-type chains (Table 1), which may similarly elevate the thermal stability of these two Hbs.…”
Section: Discussionmentioning
confidence: 90%
“…It is speculated that δ116Arg could make an extra hydrogen bond at the α 1 δ 1 interface and δ126Met (H4) could make intra-subunit non-polar contacts with δ11Val of helix A ( 45 ). However, a recent X-ray crystal studies of Hb A 2 did not support these additional interactions, but non-polar contacts formed between helices A and H were found, which presumably contribute to the higher thermal stability of Hb A 2 ( 10, 46 ). Notably, rHb WM and rHb AE, also possess Arg at position 116 of their β-type chains (Table 1), which may similarly elevate the thermal stability of these two Hbs.…”
Section: Discussionmentioning
confidence: 90%
“…HbE and HbA 2 FC were crystallized in space groups P2 1 2 1 2 1 and P2 1 , respectively, using polyethylene glycol (PEG) as a precipitant. Crystallizations and data collections of HbE and HbA 2 FC up to 1.73 and 2.1 Å resolution were reported by us previously (18). Later, the diffraction data of HbA 2 FC were collected up to 1.88 Å resolution (Table 2).…”
Section: Methodsmentioning
confidence: 98%
“…Purification, Crystallization, and Data Collection. HbA 2 and HbE were purified from the blood samples of thalassemic patients, taken for diagnosis to Thalassemia Hospital, using cation exchange column chromatography, as elaborated in our previous work (18). HbE was purified from the E/βthalassemic blood samples containing a high level of HbE, whereas HbA 2 was purified from the blood samples of β-thalassemia minor patients.…”
Section: Methodsmentioning
confidence: 99%
“…The R2 structure 1BBB has a high average R2-DSS and a low T-DSS, as expected. The two proteins that show the highest average R2-DSS are Hb-E structures 1YVQ and 1NQP [71]. The two structures are in the bound state, the former binds to CO and the latter to CN and was also reported to represent the R2 state.…”
Section: Resultsmentioning
confidence: 99%