CSDC2, a cold shock domain RNA‐binding protein in decidualization

Vallejo, Griselda; Mestre-Citrinovitz, Ana C.; Winterhager, Elke; Saragüeta, Patricia

doi:10.1002/jcp.26885

Cited by 7 publications

(5 citation statements)

References 18 publications

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“…The mice ( n = 5) were anesthetized with 0.2 ml 10% chloral hydrate on D3, skin incisions were made in the dorsal midline, and two small incisions were made in the muscle wall near the ovary to expose the uterine-oviduct connecting region. Mus-GLUT1 siRNA (80 pmol; sense, 5'-GCUUAUGGGCUUCUCCAAATT-3'; antisense, 5'-UUUGGAGAAGCCCAUAAGCTT-3'), or a scrambled siRNA negative control (NC; sense, 5'-UUCUCCGAACGUGUCACGUTT-3'; antisense, 5'-ACGUGACACGYYCGGAGAATT-3'; GenePharma, Shanghai, China) was mixed with lipofectamine 2000 (5 μl) and saline to 20 μl and injected into the lumen of each uterine horn at the uterine-oviduct connecting region ( Bagot et al, 2000 ; Guo et al, 2014 ; Vallejo et al, 2018 ). The uterine tissue was collected on D7, and the number of implanted embryos was determined.…”

Section: Methodsmentioning

confidence: 99%

Progesterone Regulates Glucose Metabolism Through Glucose Transporter 1 to Promote Endometrial Receptivity

et al. 2020

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Successful embryo implantation requires receptive endometrium, which is conducive to the process of embryo recognition, adhesion, and invasion within a certain period of time and is inseparable from the dynamic interaction between 17β-estradiol (E2) and progesterone (P4). Proper glucose metabolism is critical for the profound physiological changes in the endometrium entering the receptive state. And glucose transporters (GLUTs) are responsible for intracellular uptake of glucose and are the first step in glucose metabolism. Prior literature has reported the presence of GLUTs in the endometrium. However, we still do not understand the specific mechanisms of this process. In this study, we identified the effect of P4 on glucose transporter 1 (GLUT1) using in vivo animal models and determined the regulation of glucose metabolism by P4 in cells. We highly suspect that this pregnancy failure may be due to reduced GLUT1-mediated glucose metabolism, resulting in a decrease in endometrial receptivity caused by an inadequate energy supply and synthesis of substrate. Here, we propose a possible mechanism to explain how embryo implantation is affected by P4 and glucose utilization under abnormal endometrial conditions.

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Section: Methodsmentioning

confidence: 99%

Progesterone Regulates Glucose Metabolism Through Glucose Transporter 1 to Promote Endometrial Receptivity

et al. 2020

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“…We tend to pay attention to the TFs with the consistent expression level and the average FPKM was greater than 5 in the siGOLPH3 samples. Through literature research, it is found that the five genes in play very important roles in the process of endometrial decidualization, including ID1 ( Deepak et al, 2020 ), GATA2 ( Mazur et al, 2015 ), CREB3L1 ( Ahn et al, 2016 ), CSDC2 ( Mazur et al, 2015 ; Vallejo et al, 2018 ), and FOSL2 ( Mazur et al, 2015 ) ( Fig. 2C ).…”

Section: Resultsmentioning

confidence: 99%

“…ChIP-seq and RNA-seq experiments in hESCs before and after ecdysis in vitro showed that GATA2 could bind to PGR and affect the expression of a large number of genes, regulate decidualization of endometrial stromal cells, especially through WNT activation and stem cell differentiation ( Kohlmeier et al, 2020 ). Intrauterine siRNA injection can inhibit the abnormal expression of CSDC2 in early pregnancy; compared with control siRNA injected animals, atrial area expansion and M dysplasia were produced ( Vallejo et al, 2018 ). Both mice and humans require CREB3L1 for decidualization, the gene that targets the progesterone receptor (PR) ( Ahn et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

GOLPH3 modulates expression and alternative splicing of transcription factors associated with endometrial decidualization in human endometrial stromal cells

Zhu

Lin

et al. 2023

PeerJ

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Endometrial decidualization is a decidual tissue formed by the proliferation and re-differentiation of endometrial stroma stimulated by decidualization inducing factors. It is very important for the proper maintenance of pregnancy. Previous studies speculated that Golgi phosphoprotein 3 (GOLPH3) may have a regulatory role in the process of endometrial decidualization, while the specific molecular mechanisms of GOLPH3 is unclear. In this part, GOLPH3 was silenced in human endometrial stromal cells (hESCs), and the transcriptome data (RNA-seq) by GOLPH3 knockdown (siGOLPH3) was obtained by high-throughput sequencing technology so as to analyze the potential targets of GOLPH3 at expression and alternative splicing levels in hESCs. Through bioinformatics analysis, we found that siGOLPH3 can significantly affect the overall transcriptional level of hESCs. A total of 6,025 differentially expressed genes (DEGs) and 4,131 differentially alternative splicing events (DASEs) were identified. Through functional cluster analysis of these DEGs and genes where differential alternative splicing events are located, it is found that they are enriched in the PI3K/Akt signaling pathway, RNA splicing and processing, transcription factors and other pathways related to endometrial decidualization and important biological processes, indicating the important biological function of GOLPH3. At the same time, we focused on the analysis of the transcription factors regulated by GOLPH3, including gene expression regulation and the regulation of variable splicing. We found that GOLPH3can regulate the expression of transcription factors such as LD1, FOSL2, GATA2, CSDC2 and CREB3L1. At the same time, it affects the variable splicing mode of FOXM1 and TCF3. The function of these transcription factors is directly related to decidualization of endometrium. Therefore, we infer that GOLPH3 may participate in endometrial de membrane by regulating expression and alternative splicing levels of transcription factors. We further identified the role of GOLPH3 in the transcriptional mechanism. At the same time, it also expands the function mode of GOLPH3 protein molecule, and provides a theoretical basis for downstream targeted drug research and development and clinical application.

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“…CSDC2 is an mRNA-binding protein and a target gene of miR-373 (56). CSDC2 plays a key role in decidua development (57). However, its role in cancer development remains unknown.…”

Section: Discussionmentioning

confidence: 99%

Identification of key genes involved in the development and progression of early‑onset colorectal cancer by co‑expression network analysis

Yang

et al. 2019

Oncol Lett

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A number of studies have revealed that there is an increasing incidence of early-onset colorectal cancer (CRC) in young adults (before the age of 50 years) and a progressive decline in CRC among older patients, after the age of 50 years (late-onset CRC). However, the etiology of early-onset CRC is not fully understood. The aim of the present study was to identify key genes associated with the development of early-onset CRC through weighted gene co-expression network analysis (WGCNA). The GSE39582 dataset was downloaded from the Gene Expression Omnibus database, and the data profiles of tissues from patients diagnosed before the age of 50 years were selected. The top 10,000 genes with the highest variability were used to construct the WGCNA. Hub genes were identified from the modules associated with clinical traits using gene significance >0.2 and module membership >0.8 as the cutoff criteria. Gene Ontology and pathway analyses were subsequently performed on the hub genes and a protein-protein interaction network (PPI) was constructed. The diagnostic value of module hub genes with a degree score >5 in the PPI network was verified in samples from patients with CRC diagnosed before the age of 50 years obtained from The Cancer Genome Atlas. Eight co-expressed gene modules were identified in the WGCNA and two modules (blue and turquoise) were associated with the tumor-node-metastasis stage. A total of 140 module hub genes were identified and found to be enriched in 'mitochondrial large ribosomal subunit', 'structural constituent of ribosome', 'poly (A) RNA binding', 'collagen binding', 'protein ubiquitination' and 'ribosome pathway'. Twenty-six module hub genes were found to have a degree score >5 in the PPI network, seven of which [secreted protein acidic and cysteine rich (SPARC), decorin (DCN), fibrillin 1 (FBN1), WW domain containing transcription regulator 1 (WWTR1), transgelin (TAGLN), DEAD-box helicase 28 (DDX28) and cold shock domain containing C2 (CSDC2)], had good prognostic values for patients with early-onset CRC, but not late-onset CRC. Therefore, SPARC, DCN, FBN1, WWTR1, TAGLN, DDX28 and CSDC2 may contribute to the development of early-onset CRC and may serve as potential diagnostic biomarkers.

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CSDC2, a cold shock domain RNA‐binding protein in decidualization

Cited by 7 publications

References 18 publications

Progesterone Regulates Glucose Metabolism Through Glucose Transporter 1 to Promote Endometrial Receptivity

Progesterone Regulates Glucose Metabolism Through Glucose Transporter 1 to Promote Endometrial Receptivity

GOLPH3 modulates expression and alternative splicing of transcription factors associated with endometrial decidualization in human endometrial stromal cells

Identification of key genes involved in the development and progression of early‑onset colorectal cancer by co‑expression network analysis

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