CSF Protein Concentration Shows No Correlation With Brain Volume Measures

Wuschek, Alexander; Grahl, Sophia; Pongratz, Viola; Korn, Thomas; Kirschke, Jan S.; Zimmer, Claus; Hemmer, Bernhard; Mühlau, Mark

doi:10.3389/fneur.2019.00463

Cited by 2 publications

(2 citation statements)

References 8 publications

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“…First, the protein extractions of the included samples were characterised. Although measured CSF protein concentrations are often lower than measured in this study (Wuschek et al, 2019;Yoshihara et al, 2021), extensive individual variability has been reported previously (Asgari et al, 2017). In order to check if there was a correlation between sample storage time and protein concentration, linear regression was performed.…”

Section:  Discussionmentioning

confidence: 81%

Proteomic comparison between non‐purified cerebrospinal fluid and cerebrospinal fluid‐derived extracellular vesicles from patients with Alzheimer's, Parkinson's and Lewy body dementia

Hirschberg,

Valle‐Tamayo,

Dols‐Icardo

et al. 2023

J of Extracellular Vesicle

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Dementia is a leading cause of death worldwide, with increasing prevalence as global life expectancy increases. The most common neurodegenerative disorders are Alzheimer's disease (AD), dementia with Lewy bodies (DLB) and Parkinson's disease dementia (PDD). With this study, we took an in‐depth look at the proteome of the (non‐purified) cerebrospinal fluid (CSF) and the CSF‐derived extracellular vesicles (EVs) of AD, PD, PD‐MCI (Parkinson's disease with mild cognitive impairment), PDD and DLB patients analysed by label‐free mass spectrometry. This has led to the discovery of differentially expressed proteins that may be helpful for differential diagnosis. We observed a greater number of differentially expressed proteins in CSF‐derived EV samples (N = 276) compared to non‐purified CSF (N = 169), with minimal overlap between both datasets. This finding suggests that CSF‐derived EV samples may be more suitable for the discovery phase of a biomarker study, due to the removal of more abundant proteins, resulting in a narrower dynamic range. As disease‐specific markers, we selected a total of 39 biomarker candidates identified in non‐purified CSF, and 37 biomarker candidates across the different diseases under investigation in the CSF‐derived EV data. After further exploration and validation of these proteins, they can be used to further differentiate between the included dementias and may offer new avenues for research into more disease‐specific pharmacological therapeutics.

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Section:  Discussionmentioning

confidence: 81%

Proteomic comparison between non‐purified cerebrospinal fluid and cerebrospinal fluid‐derived extracellular vesicles from patients with Alzheimer's, Parkinson's and Lewy body dementia

Hirschberg,

Valle‐Tamayo,

Dols‐Icardo

et al. 2023

J of Extracellular Vesicle

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show abstract

“…While it is common for tear biomarkers to express their concentrations per unit protein (pg/mg protein), this is usually not done for CSF biomarkers. However, CSF protein concentrations vary greatly amongst individuals (26) and can be elevated after for example ischemic stroke (27).…”

Section: Discussionmentioning

confidence: 99%

Detection of amyloid-beta and tau in tear fluid of patients with Alzheimer’s disease

Gijs

Ramakers

Visser

et al. 2020

Preprint

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Background There is growing interest in finding non-invasive alternatives to cerebrospinal fluid (CSF) that could serve as front-line diagnostics for Alzheimer’s disease (AD). In this study, we investigated AD-specific biomarkers in tear fluid.Methods Tear fluid was collected from 25 patients with subjective cognitive decline SCD), 24 patients with mild cognitive impairment (MCI), 11 dementia patients and nine controls. Amyloid-beta peptides (AB38, AB40, AB42), t-tau and p-tau levels in tear fluid were determined using multiplex immunoassays.Results Tear t-tau levels in dementia, MCI and SCD patients were higher than in HC (p = 0.002, p = 0.002 and p = 0.013, respectively). A negative correlation between AB42 and t-tau was found in both tear fluid and CSF. Levels of tear p-tau were detectable in patients but not in HC.Conclusions This study shows for the first time presence of amyloid-beta peptides (AB38, AB40, AB42), t-tau and p-tau in tear fluid.

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CSF Protein Concentration Shows No Correlation With Brain Volume Measures

Cited by 2 publications

References 8 publications

Proteomic comparison between non‐purified cerebrospinal fluid and cerebrospinal fluid‐derived extracellular vesicles from patients with Alzheimer's, Parkinson's and Lewy body dementia

Proteomic comparison between non‐purified cerebrospinal fluid and cerebrospinal fluid‐derived extracellular vesicles from patients with Alzheimer's, Parkinson's and Lewy body dementia

Detection of amyloid-beta and tau in tear fluid of patients with Alzheimer’s disease

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