2005
DOI: 10.1093/nar/gki989
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CTCF binds the proximal exonic region of hTERT and inhibits its transcription

Abstract: The expression of the catalytic subunit (hTERT) represents the limiting factor for telomerase activity. Previously, we detected a transcriptional repressor effect of the proximal exonic region (first two exons) of the hTERT gene. To better understand the mechanism involved and to identify a potential repressor, we further characterized this region. The addition of the hTERT proximal exonic region downstream of the hTERT minimal promoter strongly reduced promoter transcriptional activity in all cells tested (tu… Show more

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Cited by 120 publications
(148 citation statements)
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“…In keeping, earlier studies showed that methylation of the hTERT promoter at the CTCF-binding site inhibited the binding of CTCF, thus derepressing hTERT in some human tumors. 16,17 Previous reports showed that a region between À578 and À300 bp upstream of the ATG initiation codon in the hTERT promoter, which corresponds to the domain identified in our study, 20,52 functions as a transcriptional silencer. This region includes MZF-2 and WT1 binding sites.…”
Section: Discussionsupporting
confidence: 68%
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“…In keeping, earlier studies showed that methylation of the hTERT promoter at the CTCF-binding site inhibited the binding of CTCF, thus derepressing hTERT in some human tumors. 16,17 Previous reports showed that a region between À578 and À300 bp upstream of the ATG initiation codon in the hTERT promoter, which corresponds to the domain identified in our study, 20,52 functions as a transcriptional silencer. This region includes MZF-2 and WT1 binding sites.…”
Section: Discussionsupporting
confidence: 68%
“…16,17 Thus, it was not surprising to observe constitutive binding of CTCF to this proximal region in the cells used in this study, as this region is unmethylated in both NB4-LR1 and NB4-LR1 SFD variants. However, through this hTERT regulation by retinoids in maturation-resistant APL cells A Azouz et al binding, CTCF has been described as a repressor of hTERT.…”
Section: Discussionmentioning
confidence: 72%
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“…There were also several genes that were repressed by decitabine treatment in a p53-independent manner, suggesting that other transcriptional repressors may be similarly regulated. Earlier studies showed that methylation of the hTERT promoter at the CTCF-binding site inhibited the binding of CTCF, thus, de-repressing hTert in human tumors (Renaud et al, 2005(Renaud et al, , 2007. Similarly, binding of various E2F transcription factors was shown to be inhibited by DNA methylation in a promoterspecific context (Campanero et al, 2000).…”
mentioning
confidence: 99%