CTTTA Deletion/Insertion polymorphism in 3'-UTR of LEPR gene in type 2 diabetes subjects belonging to Kashmiri population

Hameed, Iqra; Masoodi, Shariq Rashid; Afroze, Dil; Bhat, Riyaz Ahmad; Naykoo, Niyaz A.; Mir, Shabir Ahmad; Mubarik, Idrees; Ganai, Bashir Ahmad

doi:10.1186/s40200-014-0124-z

Cited by 5 publications

(5 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, low copy number of the salivary amylase alpha 1A ( AMY1A ) gene has been related to a predisposition for obesity, suggesting a link between carbohydrate metabolism and obesity [40,41]. Another DNA biomarker is the pentanucleotide (CTTTA) Del/Ins variant in the 3′-UTR of the LEPR gene, which has been associated with the risk of T2DM [42]. Further studies are needed to assess possible interactions between these genetic variants and dietary intake in relation to disease risk as well as their effects on dietary response, but such investigations provide examples of the direction in which future research in the field should be headed.…”

Section: Genetic Background and Nutritional Prescriptionsmentioning

confidence: 99%

Guide for Current Nutrigenetic, Nutrigenomic, and Nutriepigenetic Approaches for Precision Nutrition Involving the Prevention and Management of Chronic Diseases Associated with Obesity

Ramos-López

Milagro

Allayee³

et al. 2017

Lifestyle Genomics

139

View full text Add to dashboard Cite

Chronic diseases, including obesity, are major causes of morbidity and mortality in most countries. The adverse impacts of obesity and associated comorbidities on health remain a major concern due to the lack of effective interventions for prevention and management. Precision nutrition is an emerging therapeutic approach that takes into account an individual's genetic and epigenetic information, as well as age, gender, or particular physiopathological status. Advances in genomic sciences are contributing to a better understanding of the role of genetic variants and epigenetic signatures as well as gene expression patterns in the development of diverse chronic conditions, and how they may modify therapeutic responses. This knowledge has led to the search for genetic and epigenetic biomarkers to predict the risk of developing chronic diseases and personalizing their prevention and treatment. Additionally, original nutritional interventions based on nutrients and bioactive dietary compounds that can modify epigenetic marks and gene expression have been implemented. Although caution must be exercised, these scientific insights are paving the way for the design of innovative strategies for the control of chronic diseases accompanying obesity. This document provides a number of examples of the huge potential of understanding nutrigenetic, nutrigenomic, and nutriepigenetic roles in precision nutrition.

show abstract

Section: Genetic Background and Nutritional Prescriptionsmentioning

confidence: 99%

Guide for Current Nutrigenetic, Nutrigenomic, and Nutriepigenetic Approaches for Precision Nutrition Involving the Prevention and Management of Chronic Diseases Associated with Obesity

Ramos-López

Milagro

Allayee³

et al. 2017

Lifestyle Genomics

139

View full text Add to dashboard Cite

show abstract

“…In addition, carriers of the Ins allele had a reduced risk of diabetes compared with non-carriers (25). Similarly, in other groups, the Ins allele of the LEPR Del/Ins polymorphism is associated with a reduced risk of development of diabetes in Mexican (27) and Kashmiri (28) populations. These results suggest that alterations in the leptin signaling system can contribute to serum insulin levels and the development of T2DM.…”

Section: Discussionmentioning

confidence: 77%

“…172R-1000; Intron Biotechnology, Inc.). Genotyping of LEPR Del/Ins, miR-27a (rs895819) and miR-155 (rs767649) polymorphisms were performed using a PCR and restriction fragment length polymorphism (RFLP) technique, as described in previous papers (28)(29)(30). Restriction enzyme digestion was performed at 37˚C for 17 h with RsaI, DraIII and Tsp45I for LEPR Del/Ins, miR-27a (rs895819) and miR-155 (rs767649) polymorphisms, respectively (New England BioLabs, Inc.).…”

Section: Methodsmentioning

confidence: 99%

“…Del/Ins polymorphism in the LEPR gene has mainly been reported to be associated with susceptibility to T2DM (19,(22)(23)(24)(25)(26)(27), although this remains controversial. Previous studies have also suggested that miR-27a and miR-155 play an important role in the regulation of LEPR expression (28,29). These miRNAs have also been found to be differentially expressed in various diseases.…”

Section: Introductionmentioning

confidence: 92%

See 1 more Smart Citation

Associations of leptin receptors and miRNA polymorphisms with susceptibility to hypertension

Kim,

Hong

2023

Biomed Rep

View full text Add to dashboard Cite

Leptin receptors (LEPR) are located in the central nervous system and other tissues including adipocytes and endothelial cells, where they play a key role in mediating the effects of leptin. MicroRNA (miR/miRNA)-27a and miR-155 have been shown to play an important role in the regulation of LEPR expression and are differentially expressed in various diseases. Therefore, the present study analyzed potential associations of LEPR deletion/insertion (Del/Ins), miR-27aA>G (rs895819) and miR-155T>A (rs767649) polymorphisms with a predisposition to hypertension (HTN). Genotyping was performed by a PCR-restriction fragment length polymorphism assay. Frequencies of LEPR Del/Ins and miRNA gene polymorphisms in patients diagnosed with HTN (n=232) and randomly selected healthy controls (n=247) were assessed. The present study found that Del/Ins and Ins/Ins genotypes and the Ins allele of the LEPR Del/Ins polymorphism were associated with a decreased risk of HTN compared with controls, whereas the miR-27aA>G rs895819 polymorphism was associated with an increased risk of HTN. Combined genotype and allele analyses for LEPR Del/Ins and two miRNA polymorphisms revealed an association with an increased risk or a decreased risk of HTN. Furthermore, stratification analysis revealed that HTN risk factors were associated with waist circumference (WC) and high-density lipoprotein cholesterol (HDL-C) values in LEPR Del/Ins polymorphism. They were also associated with body mass index, WC, triglyceride and HDL-C values in miR-27aA>G polymorphism. The present study revealed a combined effect of LEPR Del/Ins and miR-27aA>G polymorphisms on the risk of HTN in Koreans, suggesting that these gene polymorphisms could be potential markers for predicting HTN risk.

show abstract

“…T2DM is described as a polygenic disease, often clinically called IR syndrome; the disease is closely related to hypothyroidism, hypertension, obesity, and lipid disorders [8]. Various research studies have attempted to discover the polymorphisms of important genes which are potentially involved in major human diseases and such studies provide valuable information about their genetic role in the progression of diseases [22], but the studies exploring the genetic vari- ations or molecular mechanisms involved in the etiopathogenesis of T2DM in this population have been very meagre [23].…”

Section: Discussionmentioning

confidence: 99%

Implication of Leptin and Leptin Receptor Gene Variations in Type 2 Diabetes Mellitus: A Case-Control Study

et al. 2022

View full text Add to dashboard Cite

Background: Global increase in the prevalence of type 2 diabetes mellitus (T2DM) has affected about 6% of the population and is one of the major healthcare challenges worldwide. Apart from other factors, genetics play a pivotal role in the development of diabetes. Recent studies have drawn attention to the role of leptin (LEP) and leptin receptor (LEPR) gene polymorphisms in the pathogenesis of T2DM, that being the reason for the uptake of this study.Methods: A total of 390 T2DM cases and 408 controls matched with respect to age and gender were taken for the study. Biochemical analysis was performed on all study subjects. Polymerase chain reaction (PCR) amplification of the genomic regions encompassing the single nucleotide polymorphisms (SNPs) under study was followed by digestion using specific restriction enzymes to analyze the SNP genotype through restriction fragment length polymorphism (RFLP).Results: Serum leptin levels were elevated in 57.9% (226 of 390) of cases as compared to 11.8% (48 of 408) of controls (P < 0.0001). Cases had significant homeostatic model assessment-insulin resistance (HOMA-IR) as compared to controls (5.3 ± 5.9 vs. 1.4 ± 0.4; P < 0.0001). In case of LEP G2548A SNP, the frequency of a variant genotype (GA + AA) was found to be higher for cases than controls (69.7% vs. 29.4%; P < 0.0001). For LEPR Q223R SNP, the frequency of a variant genotype (AG + GG) was found to be higher for cases than controls (69.2% vs. 23.6%; P < 0.0001). Conclusion:We observed a significant association between the LEP/ LEPR polymorphisms and T2DM in the ethnic population of Kashmir indicating that genetic susceptibility may play an important role in the pathogenesis of T2DM.

show abstract

CTTTA Deletion/Insertion polymorphism in 3'-UTR of LEPR gene in type 2 diabetes subjects belonging to Kashmiri population

Cited by 5 publications

References 40 publications

Guide for Current Nutrigenetic, Nutrigenomic, and Nutriepigenetic Approaches for Precision Nutrition Involving the Prevention and Management of Chronic Diseases Associated with Obesity

Guide for Current Nutrigenetic, Nutrigenomic, and Nutriepigenetic Approaches for Precision Nutrition Involving the Prevention and Management of Chronic Diseases Associated with Obesity

Associations of leptin receptors and miRNA polymorphisms with susceptibility to hypertension

Implication of Leptin and Leptin Receptor Gene Variations in Type 2 Diabetes Mellitus: A Case-Control Study

Contact Info

Product

Resources

About